Structure of PDB 7wtl Chain CL Binding Site BS01

Receptor Information
>7wtl Chain CL (length=610) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
GHNAKAFAVAAPGKMARTMQRSSDVNERKLHVPMVDRTPEDDPPPFIVAV
VGPPGTGKTTLIRSLVRRMTKSTLNDIQGPITVVSGKHRRLTFLECPADD
LNAMIDIAKIADLVLLLIDGNFGFEMETMEFLNIAQHHGMPRVLGVATHL
DLFKSQSTLRASKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDREILNLS
RFISVMKFRPLKWRNEHPYMLADRFTDLTHPELIETQGLQIDRKVAIYGY
LHGTPLPSAPGTRVHIAGVGDFSVAQIEKLPDPCPTPFYQQKLDDFERDK
DKLIYAPMSDVYDTWYELQKAKISKQLEINNIEYQEMTPEQRQRIEGFKA
GSYVRIVFEKVPMEFVKNFNPKFPIVMGGLLPTEIKFGIVKARLRRHRWH
KKILKTNDPLVLSLGWRRFQTLPIYTTTDSRTRTRMLKYTPEHTYCNAAF
YGPLCSPNTPFCGVQIVANSDTGNGFRIAATGIVEEIDVNIEIVKKLKLV
GFPYKIFKNTAFIKDMFSSAMEVARFEGAQIKTVSGIRGEIKRALSKPEG
HYRAAFEDKILMSDIVILRSWYPVRVKKFYNPVTSLLLKEKTEWKGLRLT
GQIRAAMNLE
Ligand information
>7wtl Chain C2 (length=1102) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauua
aaucaguuaucguuuauuugauaguuccucuacaugguauaacuguggua
auucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagaugua
uuuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaaua
acuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgg
guaacggggaauaaggguucgauuccggagagggagccugagaaacggcu
accacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauuca
gggagguagugacaauaaauaacgauacagggucuuguaauuggaaugag
uacaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcc
agcagccgcgguaauuccagcuccaauagcguauauuauguugcaguuaa
aaagcucguaguugaacuuugggcccgguuaacggggccuuuccuucugg
cuaaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcaggcgu
auugcucgaauauauuagcauggaauaauagaauaggacguuugguucua
uuuuguugguuucuaggaccaucguaaugauuaauagggacggucggggg
caucaguauucaauugucagaggugaaauucuuggauuuauugaagacua
acuacugcgaaagcauuugccaaggacguuuucauuaaucaagaacgaaa
guuaggggaucgaagaugaucagauaccgucguagucuuaaccauaaacu
augccgacuagggaucgggugguguuuuuuuaaugacccacucggcaccu
uacgagaaaucaaagucuuuggguucuggggggaguauggucgcaaggcu
gaaacuuaaaggaauugagguuuccguaggugaaccugcggaaggaucau
ua
...<<<<<.{{[[[[>>>>>[[[..((((((................[[[
.[[[..<<....<<....<<.......>>...>>.>>......{{.....
...[[[..{{..{{....[[[...............<<....<<.<<<..
...>>>.>>.....>>.........<<<<<..<<....>>..>>>>>[..
.((((((......<<<<<<<<<<<....>>>....<<......>>)))))
).....]...<<<<..<<<.....>>>.>>>>....]]]...}}}}..]]
].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>
>...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>
.>>.<.<<<..>>>.>...]]]]]].........<<<....<<<.....>
>>..>>>...............<<<<<<<<....>>>>>.>>>......<
<..<...........>..>>.........<<<<<........<<<<.<<<
.<<....>>>>>....>>>>.>>>>>..)))))).]]].....[[[{{..
.....{{...[[[.{{....<<<<<<.<<..>>.>>>>>>....<<<<<<
.....>>>>>>...<<<<<.<<.......<<...<.......>..<<<..
...>>>....>>......>>.>>..>>>.........[[[..((((((((
....>>>>>>>>.)))))))).]]].....}}....<<<<<<.<<...<<
<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>>..>>.
......<<....>>...>>>>...>>>>>.>>>...]]]...}}......
<<<<<<..<.<...<<<...........>>>...>>>>>>>>........
..<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>
...<<..}}>>...>>.....>>>.]]].<<<......<<<<....>>>>
....>>>..]]]].}}.....<<<<<<<<<....>>>>>>>>>.......
..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7wtl In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation.
Resolution3.3 Å
Binding residue
(original residue number in PDB)		G25 N27 K29 A30 F31 A32 V33 A34 A35 P36 K38 Q44 R45 R52 K53 H55 V56 S179 T182 R184 K191 H192 W195 Y199 G201 R225 K231 F232 R233 P234 Y751 K755 S865 R973
Binding residue
(residue number reindexed from 1)		G1 N3 K5 A6 F7 A8 V9 A10 A11 P12 K14 Q20 R21 R28 K29 H31 V32 S155 T158 R160 K167 H168 W171 Y175 G177 R201 K207 F208 R209 P210 Y316 K320 S430 R538
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003729 mRNA binding
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0016887 ATP hydrolysis activity
GO:0034511 U3 snoRNA binding
Biological Process
GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000479 endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0030490 maturation of SSU-rRNA
GO:0042254 ribosome biogenesis
GO:0042274 ribosomal small subunit biogenesis
GO:2000232 regulation of rRNA processing
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0030686 90S preribosome
GO:0032040 small-subunit processome

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:7wtl, PDBe:7wtl, PDBj:7wtl
PDBsum7wtl
PubMed36263816
UniProtQ08965|BMS1_YEAST Ribosome biogenesis protein BMS1 (Gene Name=BMS1)

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