Structure of PDB 8i9p Chain CE Binding Site BS01

Receptor Information
>8i9p Chain CE (length=463) Species: 759272 (Thermochaetoides thermophila DSM 1495) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
QEFSELNLSEKTTKAIAEMGFTKMTEIQRRAIPPALAGKDVLGAAKTGSG
KTLAFLIPAVEMLSSLRFKPRNGTGAIVVTPTRELALQIFGVARELMKYH
SQTYGVVIGGANRRAEAEKLGKGVNLLIATPGRLLDHLQNTPFVFKNLKS
LIIDEADRILEIGFEDEMRQIVKILPKEDRQTMLFSATQTTKVEDLARIS
LRPGPLYINVDEEKKYSTVEGLEQGYVVVEADKRFLLLFSFLKKMAKKKI
IVFFSSCNSVKYYSELLQYIDLPVLDLHGKQKQQKRTNTFFEFCNAKSGT
LICTDVAARGLDIPQVDWIVQFDPPDDPRDYIHRVGRTARGNNGKGRSLL
FLQPCELGFLAHLKAAKVPVVEYDFPKNKILNVQSQLEKLISTNYYLNQS
AKEGYRSYIHAYASHSLRSVFDVHKLDLVKVAKSFGFSTPPRVDITLGRR
AYGSQPRQGGRYK
Ligand information
>8i9p Chain C1 (length=1307) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uugaccucggaucagguaggaggacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaacagggauugcccuaguaacggcgagugaagc
ggcaacagcucaaauuugaaagcuggcuucggcccgcguuguaauuugga
gaggaugcuuugggcgaggcuccuucugaguucccuggaacgggacgcca
cagagggugagagccccguauaguuggaagccaagccuguguaaagcucc
uucgacgagucgaguaguuugggaaugcugcucaaaaugggagguaaauu
ucuucuaaagcuaaauaccggccagagaccgauagcgcacaaguagagug
aucgaaagaugaaaagcacuuugaaaagaggguuaaauagcacgugaaau
uguugaaagggaagcgcuugugaccagacuugcgcccggcggaucauccg
guguucucaccggugcacuccgccgggcucaggccagcaucgguucuggc
ggggggauaaaggcccagggaauguggcuccuccgggaguguuauagccc
uggguguaauacccucgccgggaccgaggaccgcgcucugcaaggaugcu
ggcguaauggucaccagcgacccgucuugaaacacggaccaaggagucaa
gguuuugcgcgaguguuuggguguaaaacccgcacgcguaaaggugagag
cuucggcgcaucaucgaccgauccugauguauucggauggauuugaguag
gagcguuaagccuuggacccgaaagauggugaacaaccaucuaguagcug
guuaccgccgaaguuucccucaggauagcagugucgaccuucaguuuuau
gagguaaagcgaaugaacuuuaaauauguaagaagcccuuguuacuuaac
ugaacgugggcauucgaauguaucgacacuagugggccauuuuugguaag
cagaacuggcgaugcgggaugaaccgaacgcgggguuaaggugccggagu
ggacgcucaucagacuggcgcucaagcgucccacccauaccccgcccuca
ggguagaaacgaugcccugaggaguaggcggccguggaggucagugacga
agccuagggcgugagcccgggucgaacggccucuagugcagaucuuggug
guaguagcaaauaucuagcgaaaccacagacccgcacguagacguauaag
aauaggcuccggccucguauccuagcaggcgauuccuccgccggccucga
aguggccgucgguaauucgcguauugcaaagacacgcgcgggaucaaauc
agauuuc
........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<..<<.......<<.<<<....>>>.>>.......>>....
<<<<<.<<<<<<<<<..<<<<<<..<<....<<<<......>>>>.((..
....<<<.....))>>>....>>..>>>.>>>..>>>>....>>>>>>>>
.>>........<<<<<<<........>>>>>>>.....<<<<<<......
>>>>>>..>>>..................................<<...
.>>...............<<<<....>>>>....................
..............<<<<.<<<<<<<....<<.<<<<<<<<<<.<<.<<<
<<<....>>>>>>>>...>>>>>>>>>>.>>.<<<<<<<.<<<<<<<<<<
<<<<<.......<<<<<<<<......<<<<<....>>>>>.......>>>
>>>>>......>>>>>>>>>>>>>>>........<<...>>.....>>>>
>>>....>>>>>>>.>>>>..<<<<.<....>.>>>><<<<<<...<<<<
<<<<<.<<<<..<<<...<<<<.....>>>>...>>>.....<<<<<..<
<....>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>..
..>>>>.>>>>>>>>>.<<.<..<<<<<<<.....>>>>>>...>..>.>
>...<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<<<<<<
......<<<........>>.>......>>>>...<<<..<<<<.<.....
.>>>>>.>>.>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....
>>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((.....
<<<<<<.....<<...))).>>...>>>>>>.>>>...>>>>>>><<<<<
<<<<.........>>>>>>>>>....>>>>>.<.<<<<<<<<.<<.....
.<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>><
<.....<<.........>>....>>.....<<<<<.<<.......<<...
....<<<....>>>....>>........<<<<.....<<<.<<<<<....
...>>>>>.>>>....>>>>..............>>.>>>>>...<<<<<
.>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8i9p Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.
Resolution3.0 Å
Binding residue
(original residue number in PDB)		L197 A221 N222 R224 K229 K232 N368 K390 Y505 R578 G581 R582 Y583 K584
Binding residue
(residue number reindexed from 1)		L87 A111 N112 R114 K119 K122 N258 K280 Y395 R457 G460 R461 Y462 K463
Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006364 rRNA processing
GO:0042254 ribosome biogenesis
Cellular Component
GO:0005730 nucleolus

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8i9p, PDBe:8i9p, PDBj:8i9p
PDBsum8i9p
PubMed37129998
UniProtG0RYU9

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