Structure of PDB 4v95 Chain CC Binding Site BS01

Receptor Information

>4v95 Chain CC (length=206) Species: 300852 (Thermus thermophilus HB8)

GNKIHPIGFRLGITRDWESRWYAGKKQYRHLLLEDQRIRGLLEKELYSAG
LARVDIERAADNVAVTVHVAKPGVVIGRGGERIRVLREELAKLTGKNVAL
NVQEVQNPNLSAPLVAQRVAEQIERRFAVRRAIKQAVQRVMESGAKGAKV
IVSGRIGGAEQARTEWAAQGRVPLHTLRANIDYGFALARTTYGVLGVKAY
IFLGEV

Ligand information

>4v95 Chain CA (length=1461)

uuggagaguuugauccuggcucagggugaacgcuggcggcgugccuaaga
caugcaagucgugcgggccgcguggucagcggcggacgggugaguaacgc
gugggugaccuacccggaagagggggacaacccggggaaacucgggcuaa
ucccccauguggacccgccccuugggguguguccaaagggcuuugcccgc
uuccggaugggcccgcgucccaucagcuaguuggugggguaauggcccac
caaggcgacgacggguagccggucugagaggauggccggccacaggggca
cugagacacgggccccacuccuacgggaggcagcaguuaggaaucuuccg
caaugggcgcaagccugacggagcgacgccgcuuggaggaagaagcccuu
cgggguguaaacuccugaacccgggacgaaacccccgacgaggggacuga
cgguaccgggguaauagcgccggccaacuccgugccagcagccgcgguaa
uacggagggcgcgagcguuacccggauucacugggcguaaagggcgugua
ggcggccuggggcgucccaugugaaagaccacggcucaaccgugggggag
cgugggauacgcucaggcuagacggugggagagggugguggaauucccgg
aguagcggugaaaugcgcagauaccgggaggaacgccgauggcgaaggca
gccaccugguccacccgugacgcugaggcgcgaaagcguggggagcaaac
cggauuagauacccggguaguccacgcccuaaacgaugcgcgcuaggucu
cugggucuccugggggccgaagcuaacgcguuaagcgcgccgccugggga
guacggccgcaaggcugaaacucaaaggaauugacgggggcccgcacaag
cgguggagcaugugguuuaauucgaagcaacgcgaagaaccuuaccaggc
cuugacaugcccgggugaaagccugggcacaggugcugcauggccgucgu
cagcucgugccgugagguguuggguuaagucccgcaacgagcgcaacccc
cgccguuaguugccagcgguucggccgggcacucuaacgggacugcccgc
gaaagcgggaggaaggaggggacgacgucuggucagcauggcccuuacgg
ccugggcgacacacgugcuacaaugcccacuacaaagcgaugccacccgg
caacggggagcuaaucgcaaaaaggugggcccaguucggauuggggucug
caacccgaccccaugaagccggaaucgcuaguaaucgcggaucagccaug
ccgcggugaauacguucccgggccuuguacacaccgcccgucacgccaug
ggagcgggcucuacccgaagucgccgggagccuacgggcaggcgccgagg
guagggcccgugacuggggcgaagucguaacaagguagcuguaccggaag
gugcggcugga

.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<..<<
<.<<<..<<<..<<.<<<<<..>>>>>.>>>>>......<<.......<<
<<<<<<..<<...<<<<<<<.<<<<<....<<<<<......>>>>>....
.>>>>>...<<<<<.<<<<<....>>>>>.>>>>>..<<<<...>>>>.>
>>>>>>..>>>>>>>>>><<<....<<<..<<<<<<<<.......>>>>>
>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.<<<<<.
<.........>>>>>>.<<<<....>>>>...>>>>>>>........<<<
....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<<<..
..>>>>.....>>>>....<<<<<......<.<<<<.....>>>>..>..
.....>>>>>......<<<<<(((...<<<<<.....<<.)))>>.....
..>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<<<...
<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>>>>>>....>
>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<<<<<<<
.<..<<<......>>>.....>.>>>>>>>...........<<....>>.
>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...<<...<
<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<..<<<<<
<<<<<...>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<..
....<<<<....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<
<<<<..<<<<<<<<<<......<<........>>..........<<<<<<
<......<<<<<<<<<....>>>>>>>>>.<<<.<<<..<<<<<<.....
..<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.....<<<
<.<<<<<<<...<...<<<.....>>>>.....>>>>>>>.....<<<<<
....>>>>>........>>>>.........>>>...>>>>>>>>>...>>
>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<.
...>>>>..>>....>>>.....>>>>>>>......<....<<<<<<<..
......>>>>>>>....>.....>>>>>>....<<<<<<<..........
>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<
<..<<<<<<<<<<<<<...<.<<<<....<<<....>>>.>>>>.>..>>
>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>
>>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v95 Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): G2 N3 K4 I5 K26 S154 G155 R156 E161 A163 R172 P174 H176 T177 L178 R179 T192 Y193 G194 V195 K199
• Binding residue (residue number reindexed from 1): G1 N2 K3 I4 K25 S153 G154 R155 E160 A162 R171 P173 H175 T176 L177 R178 T191 Y192 G193 V194 K198

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v95, PDBe:4v95, PDBj:4v95
• PDBsum: 4v95
• PubMed: 22422986
• UniProt: P80372|RS3_THET8 Small ribosomal subunit protein uS3 (Gene Name=rpsC)