Structure of PDB 7wtq Chain CB Binding Site BS01

Receptor Information
>7wtq Chain CB (length=35) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SAKSFILRKKELMKRRGRKVAKDSKFTGRKRRHRF
Ligand information
>7wtq Chain C2 (length=1255) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
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augcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauua
aaucaguuaucguuuauuugauaguuccucuacaugguauaacuguggua
auucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagaugua
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acuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgg
guaacggggaauaaggguucgauuccggagagggagccugagaaacggcu
accacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauuca
gggagguagugacaauaaauaacgauacagggucuuguaauuggaaugag
uacaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcc
agcagccgcgguaauuccagcuccaauagcguauauuaaaguuguugcag
uuaaaaagcucguaguugaacuuugggcccgguuaacggggccuuuccuu
cuggcuaaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcag
gcguauugcucgaauauauuagcauggaauaauagaauaggacguuuggu
ucuauuuuguugguuucuaggaccaucguaaugauuaauagggacggucg
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acuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaagaac
gaaaguuaggggaucgaagaugaucagauaccgucguagucuuaaccaua
aacuaugccgacuagggaucgggugguguuuuuuuaaugacccacucggc
accuuacgagaaaucaaagucuuuggguucuggggggaguauggucgcaa
ggcugaaacuuaaagaauugacggaagggcgcccuuuguaccgcccgucg
cuaguaccgauugaauggcuuagugaggccucaggaucugcuuagagaag
ggggcaacuccaucucagagcggagaauuuggacaaacuuggucauuuag
aggaacuaaaagucguaacaagguuuccguaggugaaccugcggaaggau
cauua
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.....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7wtq The nucleoplasmic phase of pre-40S formation prior to nuclear export.
Resolution3.7 Å
Binding residue
(original residue number in PDB)		S241 I246 R248 K249 R256 R258 K262 S264 F266 T267 G268 R269 K270 R271 R272 H273
Binding residue
(residue number reindexed from 1)		S1 I6 R8 K9 R16 R18 K22 S24 F26 T27 G28 R29 K30 R31 R32 H33
Enzymatic activity
Enzyme Commision number 2.1.1.309: 18S rRNA (guanine(1575)-N(7))-methyltransferase.
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
GO:0016435 rRNA (guanine) methyltransferase activity
Biological Process
GO:0000056 ribosomal small subunit export from nucleus
GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006364 rRNA processing
GO:0032259 methylation
GO:0070476 rRNA (guanine-N7)-methylation
Cellular Component
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0043527 tRNA methyltransferase complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:7wtq, PDBe:7wtq, PDBj:7wtq
PDBsum7wtq
PubMed36321656
UniProtP25627|BUD23_YEAST 18S rRNA (guanine(1575)-N(7))-methyltransferase (Gene Name=BUD23)

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