Structure of PDB 7wtn Chain CB Binding Site BS01

Receptor Information

>7wtn Chain CB (length=35) Species: 4932 (Saccharomyces cerevisiae)

SAKSFILRKKELMKRRGRKVAKDSKFTGRKRRHRF

Ligand information

>7wtn Chain C2 (length=1200)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaaauacagugaaacugcgaauggcucauua
aaucaguuaucguuuauuugauaguuccucuacaugguauaacuguggua
auucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagaugua
uuuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaaua
acuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacgg
guaacggggaauaaggguucgauuccggagagggagccugagaaacggcu
accacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauuca
gggagguagugacaauaaauaacgauacagggucuuguaauuggaaugag
uacaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcc
agcagccgcgguaauuccagcuccaauagcguauauuaaaguuguugcag
uuaaaaagcucguaguugaacuuugggcccgguuaacggggccuuuccuu
cuggcuaaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcag
gcguauugcucgaauauauuagcauggaauaauagaauaggacguuuggu
ucuauuuuguugguuucuaggaccaucguaaugauuaauagggacggucg
ggggcaucaguauucaauugucagaggugaaauucuuggauuuauugaag
acuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaagaac
gaaaguuaggggaucgaagaugaucagauaccgucguagucuuaaccaua
aacuaugccgacuagggaucgggugguguuuuuuuaaugacccacucggc
accuuacgagaaaucaaagucuuuggguucuggggggaguauggucgcaa
ggcugaaacuuaaaggaauugaccgcgcuaguaccgauugaauggcuuag
ugaggccucaggaucugcagagcggagaauuuggacaaacuuggucauuu
agaggaacuaaaaguagguuuccguaggugaaccugcggaaggaucauua

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<.......>>...>>.>>......{{.....
...[[[..{{..{{....[[[...............<<....<<.<<<..
...>>>.>>.....>>.........<<<<<..<<....>>..>>>>>[..
.((((((......<<<<<<<<<<<....>>>....<<......>>)))))
).....]...<<<<..<<<.....>>>.>>>>....]]]...}}}}..]]
].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>
>...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>
.>>.<.<<<..>>>.>...]]]]]].........<<<....<<<.....>
>>..>>>...............<<<<<<<<....>>>>>.>>>......<
<..<...........>..>>.........<<<<<........<<<<.<<<
.<<....>>>>>....>>>>.>>>>>..))))))]]]].........[[[
{{.......{{...[[[.{{....<<<<<<.<<..>>.>>>>>>....<<
<<<<.....>>>>>>...<<<<<.<<.......<<...<.......>..<
<<.....>>>....>>......>>.>>..>>>.........[[[..((((
(((.....>>>>>>>>..))))))).]]].....}}....<<<<<<.<<.
..<<<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>>.
.>>.......<<....>>...>>>>...>>>>>.>>>...]]]...}}..
....<<<<<<<...<...<<<<.........>>>>...>>>>>>>>....
......<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>.
.>>>...<<..}}>>...>>.....>>>.]]].<<<......<<<<....
>>>>....>>>..]]]]....<<<....<<<<.<<....<<<<<<<<.<<
<..<.<<..<...<<<<.....>>>>...>..>>.>..>>>.>>>>>>>>
...>>.>>>.>.....>>><<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtn The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): S241 K243 I246 R248 K249 R256 R258 K262 S264 F266 G268 R269 K270 R271 R272 H273
• Binding residue (residue number reindexed from 1): S1 K3 I6 R8 K9 R16 R18 K22 S24 F26 G28 R29 K30 R31 R32 H33

Enzymatic activity

• Enzyme Commision number: 2.1.1.309: 18S rRNA (guanine(1575)-N(7))-methyltransferase.

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008168 methyltransferase activity
• GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
• GO:0016435 rRNA (guanine) methyltransferase activity

Biological Process

• GO:0000056 ribosomal small subunit export from nucleus
• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0032259 methylation
• GO:0070476 rRNA (guanine-N7)-methylation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0043527 tRNA methyltransferase complex

External links

• PDB: RCSB:7wtn, PDBe:7wtn, PDBj:7wtn
• PDBsum: 7wtn
• PubMed: 36321656
• UniProt: P25627|BUD23_YEAST 18S rRNA (guanine(1575)-N(7))-methyltransferase (Gene Name=BUD23)