Structure of PDB 7zrs Chain CA Binding Site BS01

Receptor Information

>7zrs Chain CA (length=1742) Species: 4932 (Saccharomyces cerevisiae)

KYEAGSTTAMAFAGQKFTLPVGTTRMSYNTHEEIIIPAADQASNKNYLYT
KLLKISDLDHFCKTVFPYETLNQIQSLVYPVAYKTNENMLICAPTGAGKT
DIALLTIINTIKQFIDIQYDDFKVIYVAPLKALAAEIVDKFSKKLAPFNI
QVRELTGDMQLTKAEILATQVIVTTPEKWDVVTRKANGDNDLVSKVKLLI
IDEVHLLHEDRGSVIETLVARTLRQVESSQSMIRIIGLSATLPNFMDVAD
FLGVNRQIGMFYFDQSFRPKPLEQQLLGCRGKAGSRQSKENIDKVAYDKL
SEMIQRGYQVMVFVHSRKETVKSARNFIKLAESNHEVDLFAPDPIEKDKY
SRSLVKNRDKDMKEIFQFGFGIHHAGMARSDRNLTEKMFKDGAIKVLCCT
ATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGISDVIQIFGRGGRPGFG
SANGTGILCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEISLGSVT
NVDEAIEWLGYTYMFVRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAA
RRLHALQMIVFDEVSMHFIAKDLGRVSSDFYLLNESVEIFNQMCDPRATE
ADVLSMISMSSEFDGIKFREEESKELKRLSDESVECQIGSQLDTPQGKAN
VLLQAYISQTRIFDSALSSDSNYVAQNSVRICRALFLIGVNRRWGKFSNV
MLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDTKPSMEHLLELEADEL
GELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGPDFVWD
SRIHGDAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDP
LPPQVVVKVVSDTWIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSA
LQNPLIESIYPFKYFNPMQTMTFYTLYNTNENAFVGSPTGSGKTIVAELA
IWHAFKTFPGKKIVYIAPMKALVRERVDDWRKKITPVTGDKVVELTGDSL
PDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDVSLIIMDEIHLLASDR
GPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKDHGLYN
FPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIF
VASRRQTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKL
SLQFGIGLHHAGLVQKDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLV
IIKGTQFFDAKIEGYRDMDLTDILQMMGRAGRPAYDTTGTAIVYTKESKK
MFYKHFLNVGFPVESSLHKVLDDHLGAEITSGSITNKQEALDFLSWTFLF
RRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLENLRESQCVLLHGDDIV
ATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVEYNELPVR
GGEIIMNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDL
PIADYIQDTVSVLDQSLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGY
WYEDDPVSVLPGLQLRRIKDYTFSEQGFIEMTPQQKKKKLLTLEEIGRFG
YKKLLNVFDQLTFGMTESEDTKKRFVSVCQRLPVLEGMKFEEQENNEVLT
FYSKHLSSKHNNGFEVYCDKFPKIQKELWFLIGHKGDELLMIKRCQPKQM
NKEVIIHCDLFIPEEIRGEELQFSLINDALGLRYDMVHKLIS

Ligand information

>7zrs Chain 2 (length=1771)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagcuu
ggcgaaccaggacuuuuacuuugaaaaaauuagaguguucaaagcaggcg
uauugcucgaauauauuagcauggaauaauagaauaggacguuugguucu
auuuuguugguuucuaggaccaucguaaugauuaauagggacggucgggg
gcaucaguauucaauugucagaggugaaauucuuggauuuauugaagacu
aacuacugcgaaagcauuugccaaggacguuuucauuaaucaagaacgaa
aguuaggggaucgaagaugaucagauaccgucguagucuuaaccauaaac
uaugccgacuagggaucgggugguguuuuuuuaaugacccacucggcacc
uuacgagaaaucaaagucuuuggguucuggggggaguauggucgcaaggc
ugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccugcg
gcuuaauuugacucaacacggggaaacucaccagguccagacacaauaag
gauugacagauugagagcucuuucuugauuuugugggugguggugcaugg
ccguucuuaguugguggagugauuugucugcuuaauugcgauaacgaacg
agaccuuaaccuacuaaauaguggugcuagcauuugcugguuauccacuu
cuuagagggacuaucgguuucaagccgauggaaguuugaggcaauaacag
gucugugaugcccuuagacguucugggccgcacgcgcgcuacacugacgg
agccagcgagucuaaccuuggccgagaggucuugguaaucuugugaaacu
ccgucgugcuggggauagagcauuguaauuauugcucuucaacgaggaau
uccuaguaagcgcaagucaucagcuugcguugauuacgucccugcccuuu
guacacaccgcccgucgcuaguaccgauugaauggcuuagugaggccuca
ggaucugcuuagagaagggggcaacuccaucucagagcggagaauuugga
caaacuuggucauuuagaggaacuaaaagucguaacaagguuuccguagg
ugaaccugcggaaggaucauu

...<<<<<.{.((((>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..................<<....<<
.<<<.....>>>.>>.....>>.........<<<<<..<<....>>..>>
>>><.<.<<<<<<......(((.{((.<.<....>.>....<<......>
>>>>>>>.>...>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.......
...>>>.>>.<.<<<..>>>.>...>>>>>>.........<<<....<<<
.....>>>..>>>..>....>.>.....<<<<<<<<<<<<<....>>>>>
>>>>>.>>>......<<..<...........>..>>.........<<<<<
((......<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>
>>>>.........<<<[[.....<.<<...<<<.<<....<<<<<<.<<<
<..............>>>>.>>>>>>.....<<<<<..............
......>>>>>....<<<<<.<<.......<<...<.......>..<<<<
...>>>>....>>......>>.>>..>>>.........<<...<<<<<<<
<.....)).}))).>>>>>>>>..>>.....>>....<<<<<<.<<...<
<<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>>..>>
.......<<....>>...>>>>...>>>>>.>>>...>>>...>>.>...
.<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>>>.......
...<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>
>...<<..]]>>...>>.....>>>.>>>.<<<......<<<<....>>>
>....>>>..)))).}<<<<<.<<<<<<<..<<.<<<<<<..<<<.<<<<
<<......<<........>>..........<<<<<.<....<<<<<....
....<<.<<<........>>>.>>......>>>>>...<<.<<<..<<.<
<<<<<....<<<.<<<<<....>>>...<<<......>>>...>>.>>>.
...<<<<<.<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>>>.
.>>>>.>>....<<<<<<.....>>>>>>.......>>>>>....>>>.>
>>.....>>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<<<<
<<......<<.....<<..<<<<....>>>>..>>....>>.......>>
>>>>>......<....<<<<<<..........>>>>>>....>.....>>
>>>>....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>
>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<.<<..<
...<<<<<<<.<<<<.<<<<....>>>>.>>>>.>>>>>>>...>..>>.
>..>>>.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<
....>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zrs Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 4.8 Å
• Binding residue (original residue number in PDB): R577 V580 K581 R583 R604 S605 K612 H980 K982 R1027 D1031
• Binding residue (residue number reindexed from 1): R352 V355 K356 R358 R379 S380 K387 H755 K757 R802 D806

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003724 RNA helicase activity
• GO:0003729 mRNA binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006417 regulation of translation
• GO:0032790 ribosome disassembly
• GO:0051607 defense response to virus
• GO:0072344 rescue of stalled ribosome
• GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0010494 cytoplasmic stress granule
• GO:0022626 cytosolic ribosome

External links

• PDB: RCSB:7zrs, PDBe:7zrs, PDBj:7zrs
• PDBsum: 7zrs
• PubMed: 36801861
• UniProt: P53327|SLH1_YEAST RQC trigger complex helicase SLH1 (Gene Name=SLH1)