Structure of PDB 5obm Chain C4 Binding Site BS01

Receptor Information

>5obm Chain C4 (length=127) Species: 559292 (Saccharomyces cerevisiae S288C)

SQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYAA
MLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSGL
RIGRIEDVTPVPSDSTRKKGGRRGRRL

Ligand information

>5obm Chain 2 (length=1712)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguucacaugguauaacugugguaa
uucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagauguau
uuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaauaa
cuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucug
cccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacggg
uaacggggaauaaggguucgauuccggagagggagccugagaaacggcua
ccacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauucag
ggagguagugacaauaaauaacgauacagggucuuguaauuggaaugagu
acaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcca
gcagccgcgguaauuccagcuccaauagcguauauuaaaguuguugcagu
uaaaaagcucguaguugaacuuugggcccgguuggccggucggauuucca
acggggccuuuccuucuggcuaccaggacuuuuacuuugaaaaaauuaga
guguucaaagcaggcguauugcucgaauauauuagcauggaauaauagaa
uaggacguuugguucuauuuuguugguuucuaggaccaucguaaugauua
auagggacggucgggggcaucaguauucaauugucagaggugaaauucuu
ggauuuauugaagacuaacuacugcgaaagcauuugccaaggacguuuuc
auuaaucaagaacgaaaguuaggggaucgaagaugaucagauaccgucgu
agucuuaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaa
ugacccacucggcaccuuacgagaaaucaaagucuuuggguucugggggg
aguauggucgcaaggcugaaacuuaaaggaauugacggaagggcaccacc
aggaguggagccugcggcuuaauuugacucaacacggggaaacucaccag
guccagacacaauaaggauugacagauugagagcucuuucuugauuuugu
gggugguggugcauggccguucuuaguugguggagugauuugucugcuua
auugcgauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauu
ugcugguuauccacuucuuagagggacuaucgguuucaagccgauggaag
uuugaggcaauaacaggucugugaugcccuuagacguucugggccgcacg
cgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucuug
guaaucuugugaaacuccgucgugcuggggauagagcauuguaauuauug
cucuucaacgaggaauuccuaguaagcgcaagucaucagcuugcguugau
uacgucccugcccuuuguacacaccgcccgucgcuaguaccgauugaaug
gcuuagugaggccucaggaucugccagagcggagaauuuggacaaacuug
gucauuuagaggaacuaaaagucguaacaagguuuccguaggugaaccug
cggaaggaucau

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[...........<.....<<.<<....
...>>.>>......>.........<<<<<..<......>..>>>>>[...
((((((......<<<<<<<<<<<....>>>..............))))))
.....]...<<<<..<<<.....>>>.>>>>....]]]...}}}}..]]]
.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>>
...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>.
>>.<.<<....>>.>...]]]]]].........<<<....<<<.....>>
>..>>>...............<<<<<..<....>..>>.>>>......<<
<.<...........>>.>>.........<<<<<((......<<<<.....
<<..))>>.......>>>>.>>>>>..))))))]]]].........[[[{
{.......{{...[[[.[[....<<<<<<.<<<<<....<..>....>>>
>>.>>>>>>....<<<<<<...>>>>>>...<<<<<.<<.......<<..
.<.......>..<<<.....>>>....>>......>>.>>..>>>.....
....[[...((((((((....>>>>>>>>.))))))))..]].....]].
...<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>..
....>.>>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>
...]]]...}}......<<<<<<<...<...<<<<.........>>>>..
.>>>>>>>>..........<<<.<<.<<<..<<<<<<<<.<<<.......
.>>>>>>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<.
.....<<<......>>>....>>>..]]]]..<<<<<.<<<<<<<..<<.
<<<<<<..<<<.<<<<<.......<<........>>..........<<<<
<......<<<<<........<<.<<<........>>>.>>......>>>>
>...<<.<<<..<<.<<<<<<....<<<.<<<<<....>>>...<<<...
...>>>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<<<<<<...
.>>>>>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>.....
..>>>>>....>>>.>>>.....>>>>>>>.......>>>>>....>.>>
>>.>>>.....<<<<<<<......<<.....<<..<<<<....>>>>..>
>....>>.......>>>>>>>......<....<<<<<<..........>>
>>>>....>.....>>>>>>....<<<<<<<<.......>>>>>>>>...
...>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<<
<<<.<<<..<.<<......<<<<......>>>>......>>.>..>>>.>
>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<....>>>>
>>>>>.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5obm Aminoglycoside interactions and impacts on the eukaryotic ribosome.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): R18 Y20 N24 D25 F27 H29 K36 E37 T38 R41 T43 M46 K49 A50 D51 R52 E54 R84 V121 P122 S123 D124 S125 T126 R127 K129 G131 R132 R133 R135 R136
• Binding residue (residue number reindexed from 1): R8 Y10 N14 D15 F17 H19 K26 E27 T28 R31 T33 M36 K39 A40 D41 R42 E44 R74 V111 P112 S113 D114 S115 T116 R117 K119 G121 R122 R123 R125 R126

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5obm, PDBe:5obm, PDBj:5obm
• PDBsum: 5obm
• PubMed: 29208708
• UniProt: P39516|RS14B_YEAST Small ribosomal subunit protein uS11B (Gene Name=RPS14B)