Structure of PDB 8rhz Chain C Binding Site BS01

PDB	8rhz Noncanonical assembly, neddylation and chimeric cullin RING/RBR ubiquitylation by the 1.8 MDa CUL9 E3 ligase complex
Resolution	3.37 Å
Binding residue (original residue number in PDB)	C42 C45 H80
Binding residue (residue number reindexed from 1)	C18 C21 H52
Annotation score	4

Enzymatic activity

Enzyme Commision number

2.3.2.27: RING-type E3 ubiquitin transferase.
2.3.2.32: cullin-RING-type E3 NEDD8 transferase.

Gene Ontology

	Molecular Function
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016740	transferase activity
GO:0019788	NEDD8 transferase activity
GO:0031625	ubiquitin protein ligase binding
GO:0034450	ubiquitin-ubiquitin ligase activity
GO:0046872	metal ion binding
GO:0060090	molecular adaptor activity
GO:0061629	RNA polymerase II-specific DNA-binding transcription factor binding
GO:0061630	ubiquitin protein ligase activity
GO:0061663	NEDD8 ligase activity
GO:0097602	cullin family protein binding

	Biological Process
GO:0000165	MAPK cascade
GO:0000209	protein polyubiquitination
GO:0006281	DNA repair
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0006513	protein monoubiquitination
GO:0006974	DNA damage response
GO:0007283	spermatogenesis
GO:0016567	protein ubiquitination
GO:0030163	protein catabolic process
GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0032480	negative regulation of type I interferon production
GO:0034644	cellular response to UV
GO:0042110	T cell activation
GO:0043123	positive regulation of canonical NF-kappaB signal transduction
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0043687	post-translational protein modification
GO:0045116	protein neddylation
GO:0045732	positive regulation of protein catabolic process
GO:0062197	cellular response to chemical stress
GO:0070936	protein K48-linked ubiquitination
GO:0071230	cellular response to amino acid stimulus
GO:0090090	negative regulation of canonical Wnt signaling pathway
GO:0140627	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
GO:1900076	regulation of cellular response to insulin stimulus
GO:1902499	positive regulation of protein autoubiquitination
GO:1902883	negative regulation of response to oxidative stress
GO:1904263	positive regulation of TORC1 signaling

	Cellular Component
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0019005	SCF ubiquitin ligase complex
GO:0031461	cullin-RING ubiquitin ligase complex
GO:0031462	Cul2-RING ubiquitin ligase complex
GO:0031463	Cul3-RING ubiquitin ligase complex
GO:0031464	Cul4A-RING E3 ubiquitin ligase complex
GO:0031465	Cul4B-RING E3 ubiquitin ligase complex
GO:0031466	Cul5-RING ubiquitin ligase complex
GO:0031467	Cul7-RING ubiquitin ligase complex

External links

PDB	RCSB:8rhz, PDBe:8rhz, PDBj:8rhz
PDBsum	8rhz
PubMed	38605244
UniProt	P62877\|RBX1_HUMAN E3 ubiquitin-protein ligase RBX1 (Gene Name=RBX1)