Structure of PDB 8oo0 Chain C Binding Site BS01

Receptor Information

>8oo0 Chain C (length=78) Species: 209285 (Thermochaetoides thermophila)

AFSKRFEAKQQLESYISRVEEIISDPTLSLKLKRGQKDKIEQALSEAMAQ
LEIEDSTADELKKKELALKRLVTKAMAS

Ligand information

>8oo0 Chain 1 (length=3191)

guugaccucggaucagguaggaggacccgcugaacuuaagcauaucaaua
agcggaggaaaagaaaccaacagggauugcccuaguaacggcgagugaag
cggcaacagcucaaauuugaaagcuggcuucggcccgcguuguaauuugg
agaggaugcuuugggcgaggcuccuucugaguucccuggaacgggacgcc
acagagggugagagccccguauaguuggaagccaagccuguguaaagcuc
cuucgacgagucgaguaguuugggaaugcugcucaaaaugggagguaaau
uucuucuaaagcuaaauaccggccagagaccgauagcgcacaaguagagu
gaucgaaagaugaaaagcacuuugaaaagaggguuaaauagcacgugaaa
uuguugaaagggaagcgcuugugaccagacuugcgcccggcggaucaucc
gguguucucaccggugcacuccgccgggcucaggccagcaucgguucugg
cggggggauaaaggcccagggaauguggcuccuccgggaguguuauagcc
cuggguguaauacccucgccgggaccgaggaccgcgcucugcaaggaugc
uggcguaauggucaccagcgacccgucuugaaacacggaccaaggaguca
agguuuugcgcgaguguuuggguguaaaacccgcacgcguaaugaaagug
aacguaggugagagcuucggcgcaucaucgaccgauccugauguauucgg
auggauuugaguaggagcguuaagccuuggacccgaaagauggugaacua
ugcuuggauagggugaagccagaggaaacucugguggaggcucgcagcgg
uucugacgugcaaaucgaucgucaaaucugagcaugggggcgaaagacua
aucgaaccaucuaguagcugguuaccgccgaaguuucccucaggauagca
gugucgaccuucaguuuuaugagguaaagcgaaugauuagggacucgggg
gcgauuuuuagccuucauccauucucaaacuuuaaauauguaagaagccc
uuguuacuuaacugaacgugggcauucgaauguaucgacacuagugggcc
auuuuugguaagcagaacuggcgaugcgggaugaaccgaacgcgggguua
aggugccggaguggacgcucaucagacaccacaaaaggcguuaguacauc
uugacagcaggacgguggccauggaagucggaauccgcuaaggacugugu
aacaacucaccugccgaauguacuagcccugaaaauggauggcgcucaag
cgucccacccauaccccgcccucaggguagaaacgaugcccugaggagua
ggcggccguggaggucagugacgaagccuagggcgugagcccgggucgaa
cggccucuagugcagaucuuggugguaguagcaaauacuucaaugagaac
uugaaggaccgaaguggggaaagguuccaugugaacagcgguuggacaug
gguuagucgauccuaagccauagggaaguuccguuucaaaggggcacucg
ugccccguguggcgaaagggaagccgguuaauauuccggcaccuggaugu
ggguuuugcgcggcaacgcaacugaacgcggagacgacggcgggggcccc
gggcagaguucucuuuucuucuuaacggucuaucacccuggaaacaguuu
gucuggagauaggguuuaauggccggaagagcccgacacuucugucgggu
ccggugcgcucucgacgucccuugaaaauccgcgggagggaauaauucuc
acgccaggucguacucauaaccgcagcagguccccaaggugaacagccuc
ugguugauagaacaauguagauaagggaagucggcaaaauagauccguaa
cuucgggaaaaggauuggcucuaaggguugggcacguugcggcgugcggu
uaacaaccaacuuagaacugguacggacagggggaaucugacugucuaau
uaaaacauagcauugcgauggccagaaagugguguugacgcaaugugauu
ucugcccagugcucugaaugucaaagugaagaaauucaaccaagcgcggg
uaaacggcgggaguaacuaugacucucuuaagguagccaaaugccucguc
aucuaauuagugacgcgcaugaauggauuaacgagauucccacugucccu
aucuacuaucuagcgaaaccacagccaagggaacgggcuuggcaaaauca
gcggggaaagaagacccuguugagcuugacucuaguuugacauugugaaa
agacauaggagcuccuauuguuuuuuuacuuauucaaugaagcggggcug
gacuugcguccaacuucuggaguuaagguccuucgcgggccgacccgggu
ugaagacauugucagguggggaguuuggcuggggcggcacaucuguuaaa
ccauaacgcagguguccuaaggggggcucauggagaacagaaaucuccag
uagaacaaaaggguaaaaguccccuugauuuugauuuucagugugaauac
aaaccaugaaaguguggccuaucgauccuuuagucccucgaaauuugagg
cuagaggugccagaaaaguuaccacagggauaacuggcuuguggcggcca
agcguucauagcgacgucgcuuuuugauccuucgaugucggcucuuccua
ucauaccgaagcagaauucgguaagcguuggauuguucacccacuaauag
ggaacgugagcuggguuuagaccgucgugagacagguuaguuuuacccua
cugaugaacucgucgcaaugguaauucagcuuaguacgagaggaaccgcu
gauucagauaauugguuuuugcgguuguccgaccgggcagugccgcgaag
cuaccaucugcuggauaauggcugaacgccucuaagucagaauccaugcc
agaacgcgacgauacuacccgcacguuguagacguauaagaauaggcucc
ggccucguauccuagcaggcgauuccuccgccggccucgaaguggccguc
gguaauucgcguauugcaauuuagacacgcgcgggaucaaauccuuugca
gacgacuuagaugugcgaaaggguccuguaagcaguagaguagccuuguu
guuacgaucugcugaggguaagcccuccuucgccuagauuu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>...<<<<......<<.....>>....
.>>>>...[[[..<<.......<<.<<<....>>>.>>.......>>...
.<<<<<.<<<<<<<<<..<<<<<<..<<....<<<<......>>>>.((.
.....<<<.....))>>>....>>..>>>.>>>..>>>>....>>>>>>>
>.>>........<<<<<<<........>>>>>>>.....(((((.<<<<<
<<)))))..]]]..................................<<..
..>>...............<<<<....>>>>...................
...............<<<<.<<<<<<.....<<.<<<<<<<<<.....<<
<<<<....>>>>>>......>>>>>>>>>.>>.<<<<<<<.<<<<<<<<<
<<<<<<.....<.<<<<<<<<......<<<<<....>>>>>.......>>
>>>>>>....>.>>>>>>>>>>>>>>>........<<...>>.....>>>
>>>>.....>>>>>>.>>>>..<<<<........>>>><<<<<<....<<
<<<<<<.<<<<..<<<...<<<<.....>>>>...>>>...<<....>>.
......<<<<<..<<<..>>>...>>>>>.<<<<<<<<...<<....>>.
..>>>.>>>.>>....>>>>.>>>>>>>>..<<.<...<<<<<<.....<
<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<.
.<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>.
.....>>>>>>......>.>>...<<<<<....<.<<<....>>>.><<<
<<<<<<.....<<.<<<<<<<.....<<<....<<<....<<<...<<<<
<<........>>>>>>.>>>....>>>..>>.>.....>>>>>...<<<<
.<<<<.<......>>>>>.>>>>.....>>>>...>>>>>>>.>>..<<<
<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..
.<<<(((.....<<<<<<.....<<..........<<<.<<<<<<<<<<.
......<<<<<.<<<...<<.........>>...>>>.........<<..
....>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>
>>>>>.>>>...>>>>>>><<<<<<<<<.........>>>>>>>>>....
>>>>>.<.<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>
>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<.......
>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<<<<...<<<...<<<....>>>........<<<<<.....
.>>>>>....>>>....>>...<<<<<........>>>>>))<<<<.<<<
<.<<<....<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<
<<<.<<<<.......>>>>.><...<<<<<....<<<<<<....<<<...
..>>>....>>>>>>.....>>>>>....><<<<<<<......>>>>>>>
>>>>...>>>>>>>.>>>>.........>>>>>>.........>>>...>
>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>.
.>>>>..<<<<.....<<<<<<<.<<.........<.....<<<<<....
<<..>>.....>>>>><<.<<<<<<<<<<<.<<<<<<....>>>>>>...
...>>>>>..>>>>>>.>>............<<<<<<<<<..<<<.....
....>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>.<.<
<..<<<..<<<<<<....<<<....<<<<<....>>>>.>...>>>.>>>
>..>>>>>.<<<<<.......>>>>>.........<...........<<<
<<.......>>>>>.....>.....>>.>..>>.>>>>>>>..>..>>.>
>>>>>>..>>>>>>....>>....<<<<<........>>>>>....[[[[
[..{{{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<
<<<<<<<<<<..>>>>>.>>>>>>>>>>>...<<<<<.....<<<<..<<
<<<....>>>>>..............<<<<<.....>>>>>..>>>>..>
>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<....
.......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.
....<<...(((>>....>>>>>>>...<<.<<<<<<.......>>>>.>
>>>.<<<....>>>.>>))).......((((((..(((..>>>>>>>)))
))))))..........]]]]..}}]]]].......<<.<.<.<<<<<<..
.<<.......>>...>>>>>>...>>.>.>.......<<<<<..<<<<<.
.<.<<<<<<<......>>>>>>..>.>.<<<.........>>>.....>>
>>>......>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.
]]]]]....<<<<<<<..<<<<.<.<<<<<.................>>>
>>.><<<<<...<<<<....<<<<<<<<<<....>>>>>..>>>>>...>
>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>
>....>>>>>>>.....<<<<<.<..<<<......<<.......<<<<..
>>>>....>>........<<<<.....<<<.<<<<<.......>>>>>.>
>>....>>>>..............>>>.>.>>>>>...<<<<<<<.....
........>>....<<<<<<<<<.<<<....<<<<.<..<<<<<......
>>>>>..>>>>>...>>>....>>>>..>>>>>...>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8oo0 Methionine aminopeptidase 2 and its autoproteolysis product have different binding sites on the ribosome.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K568 K574 E581 K598 R605 K609
• Binding residue (residue number reindexed from 1): K33 K39 E46 K63 R70 K74

Enzymatic activity

• Enzyme Commision number: 3.6.4.10: non-chaperonin molecular chaperone ATPase.

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0140662 ATP-dependent protein folding chaperone

Biological Process

• GO:0006412 translation
• GO:0006457 protein folding

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005886 plasma membrane

External links

• PDB: RCSB:8oo0, PDBe:8oo0, PDBj:8oo0
• PDBsum: 8oo0
• PubMed: 38267453
• UniProt: G0SCU5|SSB1_CHATD Ribosome-associated molecular chaperone SSB1 (Gene Name=SSB1)