Structure of PDB 8cai Chain C Binding Site BS01

Receptor Information

>8cai Chain C (length=64) Species: 679895 (Escherichia coli BW25113)

GQKTSQLERRVMFRRAMKAKGIKVEVSGRLGGAEIARTEWYREGRVPLHS
EAHTTYGVIGVKVW

Ligand information

>8cai Chain A (length=1114)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacagguugcugacgaguggcggacggguga
guaaugucugggaaacugccugauggagggggauaacuacuggaaacggu
agcuaauaccgcauaacgucgcaagaccaaagagcuugccaucggaugug
cccagaugggauuagcuaguaggugggguaacggcucaccuaggcgacga
ucccuagcuggucugagaggaugaccagccacacuggaacugagacacgg
uccagacuccuacgggaggcagcaguggggaauauugcacaaugggcgca
agccugaugcagccaugccgcguguaugaagaaggccuucggguuguaaa
guacuuucagcggggaggaagggaguaaaguuaauaccuuugcucauuga
cguuacccgcagaagaagcaccggcuaacuccgugccagcagccgcggua
auacggagggugcaagcguuaaucggaauuacugggcguaaagcgcacgc
aggcgguuuguuaagucagaugugaaauccccgggcucaaccugggaacu
gcaucugauacuggcaagcuugagucucguagagggggguagaauuccag
guguagcggugaaaugcguagagaucuggaggaauaccgguggcgaaggc
ggcccccuggacgaagacugacgcucaggugcgaaagcguggggagcaaa
caggauuagauacccugguaguccacgccguaaacgaugucgacuuggag
guuguuggcuuccggagcuaacgcguuaagucgaccgccuggggaguacg
gccgcaagguuaaaacucaaaugaauugacgggggcccggcauggcuguc
gucagcucguguugugaaauguuggguuaagucccgcaacgagcgcgacg
ucaaguggcuucccgggccuuguacacaccgcccgucacaccaugggagu
ggguugcaaaagaaguagguagcuuaaccuucgggagggcgcuuaccacu
uugugauucaugacuggggugaagucguaacaagguaaccguaggggaac
cugcgguuggauca

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<<..<<<....>>>>>..>>>>>......<<..
.....<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<......>>>
>>......>>.>.....<<<....>>>....<<<>>>.>>>>>>>..>>.
>>>>>>><<<....<<<..<<<<<<<.........>>>>>>>>>>.....
.>>>..<<<<<<<<....>>>>...>>>>.>>.<<<<<.<.........>
>>>>>.<<<<<..>>>>>...>>>>>>.........<<<....<<<<...
.>>>>..>>>..>>.>>>>>>..<<<<......<<<<....>>>>.....
>>>>...<.<<<<<......<.<<<<<<<<.......>>>>>>>>.>...
.....>>>>>....>..<<<<<(((...<<<<<.....<<.)))>>....
...>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<<<..
.<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>>>>>>....
>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<<<<<<
<<...<<<......>>>......>>>>>>>>...........<<....>>
.>>>>>>>..>>>>.>>>>...>>>...>>>>....<<<<<<...<<...
<<<<.........>>>>...>>>>>>>>..........<<<<<<.<<<<<
<<<<.>>>>>>>>>..<<..))>>.....>>>>>>.>>>.<<<......<
<<<....>>>>....>>>..)))).]<<<<<.<<<<<<.......<<...
....<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>......
...>>.........>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<
<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>>>>>>>>>...>
>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>
>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cai Structural conservation of antibiotic interaction with ribosomes.
• Resolution: 2.08 Å
• Binding residue (original residue number in PDB): G2 Q3 K4 S154 G155 R156 E161 I162 A163 W167 R169 R172 V173 L175 H176 E188 T192 Y193 G194 V195 G197 K199
• Binding residue (residue number reindexed from 1): G1 Q2 K3 S27 G28 R29 E34 I35 A36 W40 R42 R45 V46 L48 H49 E51 T55 Y56 G57 V58 G60 K62

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8cai, PDBe:8cai, PDBj:8cai
• PDBsum: 8cai
• PubMed: 37550453
• UniProt: P0A7V3|RS3_ECOLI Small ribosomal subunit protein uS3 (Gene Name=rpsC)