Structure of PDB 8c8y Chain C Binding Site BS01

Receptor Information

>8c8y Chain C (length=246) Species: 562 (Escherichia coli)

AVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITT
RHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGER
RYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKG
GQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHM
LRVLGKAGAARWRGVRPTVRGTAMQTKGKKTRSNKRTDKFIVRRRS

Ligand information

>8c8y Chain A (length=2548)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaucuaa
guaccccgaggaaaagaaaucaaccgagauucccccaguagcggcgagcg
aacggggagcagcccagagccugaaucaguguguguguuaguggaagcgu
cuggaaaggcgcgcgauacagggugacagccccguacacaaaaaugcaca
ugcugugagcucgaugaguagggcgggacacgugguauccugucugaaua
uggggggaccauccuccaaggcuaaauacuccugacugaccgauagugaa
ccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagugaaaa
agaaccugaaaccguguacguacaagcagugggagcacgcuuaggcgugu
gacugcguaccuuuuguauaaugggucagcgacuuauauucuguagcaag
guuaaccgaauaggggagccgaagggaaaccgagucuuaacugggcguua
aguugcaggguauagacccgaaacccggugaucuagccaugggcagguug
aagguuggguaacacuaacuggaggaccgaaccgacuaauguugaaaaau
uagcggaugacuuguggcugggggugaaaggccaaucaaaccgggagaua
gcugguucuccccgaaagcuauuuagguagcgccucgugaauucaucucc
ggggguagagcacuguuucggccgaugcaaacugcgaauaccggagaaug
uuaucacgggagacacacggcgggugcuaacguccgucgugaagagggaa
acaacccagaccgccagcuaaggucccaaagucaugguuaagugggaaac
gaugugggaaggcgucggccugcgcggaagauguaacggggcuaaaccau
gcaccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcgu
ucuguaagccugcgaaggugugcugugaggcaugcuggagguaucagaag
ugcgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgcc
ggaagaccaaggguuccuguccaacguuaaucggggcagggugagucgac
cccuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuc
cuguacuugguguuacugcgaaggggggacggagaaggcuauguuggccg
ggcgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauc
cggaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagca
acaaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaa
ucguaccccaaaccgacacagguggucagguagagaauaccaaggcgcuu
gagagaacucgggugaaggaacuaggcaaaauggugccguaacuucggga
gaaggcacgcugauauguaggugaggucccucgcggauggagcugaaauc
agucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugu
gcaaacacgaaaguggacguauacggugugacgccugcccgguguaaacg
gcggccgacgguccuaagguagcgaauggcguaaugauggccaggcuguc
uccacccgagacucagugaaauugaacucgcugugaagaugcaguguacc
cgcggcaagacggaaagaccccgugaaccuuuacuauagcuugacacuga
acauugagccuugauguguaggauaggugggaggcuuugaaguguggacg
ccagucugcauggagccgaccuugaaauaccacccuuuaauguuugaugu
ucuaacguugacccguaauccggguugcggacagugucugguggguaguu
ugacuggggcggucuccuccuaaagaguaacggaggagcacgaagguugg
cuaauccuggucggacaucaggagguuagugcaauggcauaagccagcuu
gacugcgagcgugacggcgcgagcaggugcgaaagcaggucauagugauc
cggugguucugaauggaagggccaucgcucaacggauaaaagguacuccg
gggucccuaucugccgugggcgcuggagaacugaggggggcugcuccuag
uacgagaggaccggaguggacgcaucacugguguucggguugucaugcca
auggcacugcccgguagcuaaaugcggaagagauaagugcugaaagcauc
uaagcacgaaacuugccccgagaugaguucucccugacccuuuaaggguc
cugaaggaacguugaagacgacgacguugauaggccggguguguaagcgc
agcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<..>...
...>>>>>.>.........<<....>>..<<<<<<.....<<.....>>.
...>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...<<<<
<......>>>>>((......<<<.....))>>>...>>>...>>>>>>>>
>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.....
.<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].........
......<<....>>....]].........<<<<.....>>>>........
.....}....>>>>>>>>>>>.......<<<<..<<<<<......>>>>>
..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<
<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>....
.>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>.
.>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<<<<
<<.....<<<...<<<.<<<..>>>.>>>..>>.>.....>>>>>>.>>>
...>>>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<..
....>>>...<<<<.<<<...<<<(((.....<<<<<<...........<
<..<<<<<..<<..>>..>>>>>.>>...........))).....>>>>>
>.>>>...>>>>>>><<<<<..<<....>>..>>>>>....>>>>>><.<
<<<<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>.
.>.....>>>>>><......<<.....<<<<<<.......>>>>>><<<<
....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.
<<<...<<<...<<<....>>>...>>>...>>>..<<<<<........>
>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<
<<....(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>
>>>>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>
>>>...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.
>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<
<<<....<<<<<<<<.<<...............<<<<<....<<..>>..
...>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>
>>>............<<<<<<<<<..<<<.........>>>..<<<<<<<
.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<....>>>
>>.<<<<.>>>>...............>>>>>..>>.>>>>>>>.....>
>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<
<<<<<<.(((.[[......<<<<.[[<<<<...<<<<.<<<.<<<<<.<<
<<<<<<.<..<<<............<<<<<...<<.......<<<..<..
....>..>>>....>>.............>>>>>...>>>..>>.>>>>>
>>...<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>..
.<<<.......>>><<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<....(((.>....>>>>>>>.
..<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>))).......
<<<<<<<.((..>>>>..))>>>>>>>.............>>>>..]]>>
>>...]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<...
............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))).
>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.........
..>>>>>....>>>.>>>>.......>>>>>>>..<<<<<.....>>>>>
<....<<..<<<<<.......>>>>>....<<<<<<<<..<<....<<<<
<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c8y Cryo-EM captures early ribosome assembly in action.
• Resolution: 3.03 Å
• Binding residue (original residue number in PDB): K6 P7 T8 S9 P10 G11 R12 R13 H14 K17 K35 K38 S39 G41 R42 N43 N44 N45 G46 R47 I48 T49 T50 R51 H52 G55 H57 K58 Q59 A60 Y61 R62 Y82 P84 R86 D97 E99 R100 M145 K149 Q152 L153 A154 R155 S156 A157 G158 T159 Y170 L175 R176 S177 E179 R181 H199 M200 V203 L204 G205 K206 A207 G208 A210 R211 W212 R216 P217 T218 V219 R220 T222 A223 M224 K252 G253 K254 T256 R257 K260 R261 T262 K264 R269 R270
• Binding residue (residue number reindexed from 1): K6 P7 T8 S9 P10 G11 R12 R13 H14 K17 K35 K38 S39 G41 R42 N43 N44 N45 G46 R47 I48 T49 T50 R51 H52 G55 H57 K58 Q59 A60 Y61 R62 Y82 P84 R86 D97 E99 R100 M145 K149 Q152 L153 A154 R155 S156 A157 G158 T159 Y170 L175 R176 S177 E179 R181 H199 M200 V203 L204 G205 K206 A207 G208 A210 R211 W212 R216 P217 T218 V219 R220 T222 A223 M224 K227 G228 K229 T231 R232 K235 R236 T237 K239 R244 R245

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016740 transferase activity
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0032297 negative regulation of DNA-templated DNA replication initiation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990082 DnaA-L2 complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8c8y, PDBe:8c8y, PDBj:8c8y
• PDBsum: 8c8y
• PubMed: 36797249
• UniProt: P60422|RL2_ECOLI Large ribosomal subunit protein uL2 (Gene Name=rplB)