Structure of PDB 7uvx Chain C Binding Site BS01

Receptor Information

>7uvx Chain C (length=271) Species: 480119 (Acinetobacter baumannii AB0057)

QKCKPTSPGRRFVEKVVHDHLHKGAPYAPLVEAKKRTGGRNNNGHITTRH
VGGGHKQHYRIVDFKRNKDGVPAVVERIEYDPNRTAHIALLKYADGERRY
IIAPKGLRAGDKVQSGNDAPIRPGNCLPLRNMPIGSTLHNVELKIGKGAQ
LARSAGASVQLLGRDGSYAIIRLRSGEMRKVHVECRAVIGEVSNQENNLR
SLGKAGAARWRGVRPTVRGMAMNPIDHPHGGGEGRNKGIQPVSPWGQKAK
GYKTRTNKRTTKMIIRDRRVK

Ligand information

>7uvx Chain A (length=2715)

gucaaguaauuaagugcaugugguggaugccuuggcagucagaggcgaug
aaagacgugauagccugcgaaaagcuccggggaggcggcaaauauccuuu
gauccggagaugucugaaugggggaacccaccuacuuuaagguagguauu
gcaacaugaauacauaguguugcaaggcgaacgaggggaagugaaacauc
ucaguacccuuaggaaaagaaaucaauugagauucccucaguagcggcga
gcgaacggggaucagcccauuaaguuauguguguuuuaguggaacgcucu
gggaagugcgaacguagagggugauauucccguacacgaaagggcacaca
uaaugaugacgaguagggcgaggcacgugaaaccuugucugaauaugggg
ggaccauccuccaaggcuaaauacuccugacugaccgauagugaaccagu
accgugagggaaaggcgaaaagaaccccugugaggggagugaaauagauc
cugaaaccgcaugcauacaagcagugggagcaccuucguggugugacugc
guaccuuuuguauaaugggucagcgacuuauauucaguagcgagguuaac
cguauaggggagccguagagaaaucgagucuuaauagggcguuuaguugc
uggguauagacccgaaaccaggcgaucuauccaugagcagguugaagguu
ggguaacacuaacuggaggaccgaacccacugucguugaaaagccagggg
augacuuguggauaggggugaaaggcuaaucaagccuggugauagcuggu
ucuccccgaaagcuauuuagguagcgccucggacgaauaccauagggggu
agagcacuguuucggcaccgaugcaaacuccgaauaccuaugaguacuau
ccgggagacagacugcgggugcuaacguccguagucaagaggaaaacaau
ccagaccgccagcuaaggccccaaaaucauaguuaagugggaaacgaugu
gggaaggcauagacagcuauagucgagucggccugcgcggaagauguaac
ggggcuaaaacuaugugccgaagcugcggauguauacuuuguauacgugg
uaggggagcguucuguaagccgaugaagguguguugagaagcaugcugga
gguaucagaagugcgaaugcugacgugaguaacgacaaaacgggugaaaa
acccguucgccgaaagaccaaggguuccaguccaacguuaaucggggcug
ggugagucgaccccuaaggcgaggccgaaaggcguagucgaugggaaaau
gguuaauauuccauuacuucuguguaaugcgaugagaggacggagaaggc
uaaaucagccuggcguugguuguccaggugaaaggauguaggcauguauc
uuaggcaaauccgggguacucuaugcugagaucugauagcaagcuguacu
ugugcagcgaagugguugaugccaugcuuccaggaaaagucucuaagcuu
caguuacacaggaaucguacccgaaaccgacacagguggucaggucgagu
agaccaaggcgcuugagagaacucugcugaaggaacuaggcaaaauggua
ccguaacuucgggagaagguacgcuguuguuggugauggaacucgcuucc
ugagcugacgacagccgcagaaaccaggccgcugcaacuguuuauuaaaa
acauagcacucugcaaacacgaaaguggacguauagggugugaugccugc
ccggugcuggaagguuaauugaugggguuagcguaagcgaagcucuugau
cgaagccccaguaaacggcggccgguccuaagguagcgaaauuccuuguc
ggguaaguuccgaccugcacgaauggcauaaugauggcggcgcugucucc
agcagaggcucagugaaaucgaaaucgcugugaagaugcaguguacccgc
ggcuagacggaaagaccccgugaaccuuuacugcagcuugacacugaacu
uugagguucuaacucugucccgugauccgggacgaggaccgugucuggug
gguaguuugacuggggcggucuccuccuaaagaguaacggaggaguacga
aggugcgcucagcguggucggaaaucacgcguagaguauaaaggcaaaag
cgcgcuuaacugcgagacccacaagucgagcagguacgaaaguaggucuu
agugauccggugguucuguauggaagggccaucgcucaacggauaaaagg
uacucuggggauaacaggcugauaccgcccaagaguucauaucgacggcg
guguuuggcaccucgaugucggcucaucucauccuggggcugaagcaggu
cccaaggguauggcuguucgccauuuaaagagguacgcgagcuggguuua
gaacgucgugagacaguucggucccuaucuaccgugggcgcuggaaauuu
gagaggaucugcuccuaguacgagaggaccagaguggacgaaccucuggu
guaccgguugugacgccagucgcaucgccggguagcuauguucggaaggg
auaaccgcugaaagcaucuaagcgggaagccuaccucaagauaagauuuc
ccuaggacuuuauguccucuaaagagccguucgagacuaggacguugaua
gguuggauguggaagcauagugauaugugaagcugaccaauacuaauugc
ucgugaggcuugacu

<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<....
..<<<<<<....(((.>>>.....<<<<<<<..<<.)))......>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>
......>>>>>.>.........<<<..>>>...<<<<<.....<<.....
>>....>>>>>...<<<......<<<<<<<<<<<<<<.<<<...<<<.<.
.....>.>>>((......<<<.....))>>>...>>>...>>>>>>>>>>
>>>>...........<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..>>>......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<.....>>>>.............
.....>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>>
...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<
<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<<...<<<<
<<......>>>>>>.>...>>>...<<<.<<<.<........>.>>>>>>
....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>.
.<<<<<<....<<<<<....>>>>>.<<<<<<<....<<<<<<<<<....
.<<<...<<<.<<<<..>>>>.>>>..>>.>.....>>>>>>.>>>...>
>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<..<<........<<<..>>>....>>..>>>>>.>>...........
)))......>>>>>>.>>>...>>>>>>><<<<<<<<...>>>>>>>>..
..>>>>>><.<<<<<...<<<......<<<<<<<<....>>>>>>>>...
>>>..>>>>>..>.....>>>>>><......<<.....<<<<<<......
.>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<<
<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<
..<<<<<<<<<<<...((((...>>>>>>....<<<<...<<<<.<<<<<
<..<<.....>>.>>>>>>....>>>>...>>>>........<<<<<...
...>>>>>....>>>>>...>>>...>>>>.........>>>>...>>..
..>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<....
.>>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<
<<.....<..>......>>>>><<<<<<<<<<<<...<<<<.....>>>>
...>>>>>>>>>>>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<
<..<<<<<<<..<<<<.......<<<<<..<<....>>...>>>>>....
...>>>>>>>>>..>>>>><<<..>>>........<...........<<<
<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>
>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<
<<.<<<<.[[......<<<<.[[<<<<..<<<<..<<<.<<<<<.<<<<<
<..>>>>>>...<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>.
..>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<..
.<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>....>
>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)))
]......<<<<<<<.((..>>>>..))>>>>>>>.............>>>
>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...>>>>
>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>
>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....
<<<<<<<....>>>.>>>>....]]..>>>>>>>>>>>....<<<<<<<.
..<<<<..<<<<<<.................>>>>>>.<<<<<...<<<<
...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.>>>>>...<<<
.<..<<<<<...........>>>>>...>>>>.>>>>.......>>>>>>
>..<<<<<.....>>>>><....<<<<<<<<<.......>>>>>....<<
<<<<<<..<<....<<<<<....>>>>>...>>...>>>>>.>>>...>>
>>..>..>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7uvx Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
• Resolution: 2.35 Å
• Binding residue (original residue number in PDB): K7 P8 S10 G12 R13 R14 F15 E35 K38 R39 R43 N44 N45 N46 H48 I49 T50 T51 R52 H53 H58 K59 Q60 H61 Y62 R63 V74 Y83 R87 K95 A97 D98 G99 R101 K147 K150 Q153 L154 A155 R156 S157 A158 L176 R177 S178 E180 R182 N201 L205 G206 K207 A208 G209 R212 W213 R217 P218 T219 V220 R221 G222 M223 A224 M225 N226 I228 H232 G235 E236 G237 R238 N239 K240 G241 Q243 P244 S246 P247 W248 Q250 A252 K253 G254 Y255 K256 T257 R258 R262 R271 V273 K274
• Binding residue (residue number reindexed from 1): K4 P5 S7 G9 R10 R11 F12 E32 K35 R36 R40 N41 N42 N43 H45 I46 T47 T48 R49 H50 H55 K56 Q57 H58 Y59 R60 V71 Y80 R84 K92 A94 D95 G96 R98 K144 K147 Q150 L151 A152 R153 S154 A155 L173 R174 S175 E177 R179 N198 L202 G203 K204 A205 G206 R209 W210 R214 P215 T216 V217 R218 G219 M220 A221 M222 N223 I225 H229 G232 E233 G234 R235 N236 K237 G238 Q240 P241 S243 P244 W245 Q247 A249 K250 G251 Y252 K253 T254 R255 R259 R268 V270 K271

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0016740 transferase activity
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7uvx, PDBe:7uvx, PDBj:7uvx
• PDBsum: 7uvx
• PubMed: 37192172
• UniProt: B7IA36|RL2_ACIB5 Large ribosomal subunit protein uL2 (Gene Name=rplB)