Structure of PDB 7p48 Chain C Binding Site BS01

Receptor Information

>7p48 Chain C (length=274) Species: 1280 (Staphylococcus aureus)

AIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRNNQGKLTV
RHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEK
RYIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKG
GQIARSAGASAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHE
LVNVGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWG
KPTLGKKTRRGKKSSDKLIVRGRK

Ligand information

>7p48 Chain A (length=2661)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaaugggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacaggaguuacaaaggacgacauuagacgaaucaucuggaaagaugaa
ucaaagaagguaauaauccuguagucgaaaauguugucucucuuccugag
uacgacggagcacgugaaauuccgucggaaucugggaggaccaucuccua
aggcuaaauacucucuagugaccgauagugaaccaguaccgugagggaaa
ggugaaaagcaccccggaaggggagugaaauagaaccugaaaccgugugc
uuacaaguagucagagcccguuaaugggugauggcgugccuuuuguagaa
ugaaccggcgaguuacgauuugaugcaagguuaagcaguaaauguggagc
cguagcgaaagcgagucugaauagggcguuuaguauuuggucguagaccc
gaaaccaggugaucuacccuuggucagguugaaguucagguaacacugaa
uggaggaccgaaccgacuuacguugaaaagugagcggaugaacugagggu
agcggagaaauuccaaucgaaccuggagauagcugguucucuccgaaaua
gcuuuagggcuagccucaagugaugauuauuggagguagagcacuguuug
gauucagacaaacuccgaaugccaauuaauuuaacuugggagucagaaca
ugggugauaagguccguguucgaaagggaaacagcccagaccaccagcua
aggccccaaaauauauguuaaguggaaaaggauguggcacugcgccgaaa
auguaccggggcuaaacauauuaccgaagcuguggauuguccggacaaug
guaggagagcguucuaagggcguugaagcaugaucguaaggacaugugga
gcgcuuagaagugagaaugccggugugaguagcgaaagacgggugagaau
cccguccaccgauugacuaagguuuccagaggaaggcucguccgcucugg
guuagucggguccuaagcugaggccgacaggcguaggcgauggauaacag
guugauauuccuguaccaccuauaucgauggggggacgcaguaggauagg
cgaagcgugcgauuggauugcacgucuaagcaguaaggcugaguauuagg
caaauccgguacucgggcugagcugugauggggagaucgagucguugauu
ucacacugccgagaaaagccucuagauauaggugcccguaccgcaaaccg
acacagguagucaagaugagaauucuaaggugagcgagcgaacucucguu
aaggaacucggcaaaaugaccccguaacuucgggagaaggggugcucuuu
aggguuaacgcccagaagagccgcagugaauaggcccaagcgacuguuua
ucaaaaacacaggucucugcuaaaccguaaggugauguauaggggcugac
gccugcccggugcuggaagguuaagaggagugguuagcgcgaagcuacga
aucgaagccccaguaaacggcggccguaacuauaacgguccuaagguagc
gaaauuccuugucggguaaguuccgacccgcacgaaaggcguaacgauuu
gggcacugucucaacgagagacucggugaaaucauaguaccugugaagau
gcagguuacccgcgacaggacggaaagaccccguggagcuuuacuguagc
cugauauugaaauucguggcuuucuaacccgcaccacuuaucgugguggg
agacagugucaggcgggcaguuugacuggggcggucgccuccuaaaaggu
aacggaggcgcucaaagguucccucagaaugguuggaaaucauucauaga
guguaaaggcauaagggagcuugacugcgagaccuacaagucgagcaggg
ucgaaagacggacuuagugauccggugguuccgcauggaagggccaucgc
ucaacggauaaaagcuaccccggggauaacaggcuuaucucccccaagag
uucacaucgacggggagguuuggcaccucgaugucggcucaucgcauccu
ggggcuguagucggucccaaggguugggcuguucgcccauuaaagcggua
cgcgagcuggguucagaacgucgugagacaguucggucccuauccgucgu
gggcguaggaaauuugagaggagcuguccuuaaccgggauggacauaccu
cugguguaccaguugucgugccaacggcauagcuggguagcuaugugugg
acgggauaagugcugaaagcaucuaagcaugaagccccccucaagaugag
auuucccaacuucgguuauaagaucccucaaagaugaugagguuaauagg
uucgagguggaagcauggugacauguggagcugacgaauacuaaucgauc
gaagacuuaau

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>.....<<<<......<<....
>>.......>>>>...........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<..<<<<.......<<<<<<<<<<.<<<...<<<<<......>>>>>(
(......<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>>>...
..<<<<<<<<.........>>>>>>>>......<<<<<..<<<<>>>>>.
.)))......]].>>>>>>>.]]...............<<....>>....
]].........<<<<....>>>>.............}....>>>>>>>>>
>>.......<<<<..<<<<......>>>>..>>>>...<.<........>
.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<.....>>>...>>
>...<<....>>..<<<<.....>>>>.....>>>>>.>>>>>>>..<<.
<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>>>
>.....>>>...<<<.<<<<<........>>>>>>>>....>>>>>>>>>
...<<<.....>>>......>>>>>>......>.>>..<<<<<<....<<
<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<...<<<<<<
<..>>>>>>>..>>.>.....>>>>>.>>>>..>>>>>>>.>>..<<<<<
<<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<..
.<<.(((.....<<<<<<...........<<..<<<<..>>>>.>>....
.......))).....>>>>>>..>>...>>>>>>><<<<<<..>>>>>>.
...>>>>>><.<<<<<<<.<<......<<<<<.<<....>>.>>>>>...
>>.>>>>>>>..>.....>>>>>><......<<....<<<<<<.......
>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>
....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>>...<<<<
<........>>>>>))<<<<<<.<<<...<<<<....<<<<..<<<..<<
<<<.<<<<<<...(((...>>>>>><<..<<<<<..<<<.<<<<<<..<<
.....>>.>>>>>>..>>>..>>>>>...>>...<..>...>>>>>...>
>>...>>>>.........>>>>..>>>.>>>>>>>>>>..>>.....<<.
.....>>>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<<
.<<...............<<<<<...............>>>>><<<<<<<
.<<<<....>>>>.>>>>>>>............<<<<<<<<<..<<<...
......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<
<<<.<<<..<<<<<<<..<<<<..<<...<<<<<<.<..>..>>>>>>..
.>>..>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<.
..........<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>
>>>>.....>>.>>>>>>>>.>>>>>>.....>.....<<<<<.......
.>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<
<<<<<<<.<<<<.<<..>>.>>>>....<<.<<<<<<.....>>>>>>>>
.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<........
...>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....
<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<
<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.
............>>>>..]]>>>>.......<<<<...<<<<<<...<<.
......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<
<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>.
.>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>
>>>....<<<<<<<...<<<<..<<<<<<<.....>>>>>>>.<<<<<..
.<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>.
..<<<.<..<<<<<...........>>>>>...>>>>.>>>>.......>
>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7p48 Sal-type ABC-F proteins: intrinsic and common mediators of pleuromutilin resistance by target protection in staphylococci.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): K4 K7 P8 I9 T10 N11 R13 R14 K31 K35 K39 K40 G42 R43 N44 N45 Q46 G47 K48 L49 T50 R52 H53 H54 G55 G56 H58 K59 R60 Q61 Y62 R63 I74 Y83 P85 R87 D98 G99 E100 K147 G149 K150 Q153 I154 A155 R156 S157 A158 K170 L176 R177 S178 E180 R182 E201 N204 V205 G206 K207 A208 G209 R210 S211 R212 W213 R217 P218 T219 V220 R221 S223 V224 M225 N228 H232 G235 E236 G237 R238 P240 I241 G242 R243 P244 P246 W250 T254 G256 K257 K258 T259 R260 R261 K263 S266 K275
• Binding residue (residue number reindexed from 1): K3 K6 P7 I8 T9 N10 R12 R13 K30 K34 K38 K39 G41 R42 N43 N44 Q45 G46 K47 L48 T49 R51 H52 H53 G54 G55 H57 K58 R59 Q60 Y61 R62 I73 Y82 P84 R86 D97 G98 E99 K146 G148 K149 Q152 I153 A154 R155 S156 A157 K169 L175 R176 S177 E179 R181 E200 N203 V204 G205 K206 A207 G208 R209 S210 R211 W212 R216 P217 T218 V219 R220 S222 V223 M224 N227 H231 G234 E235 G236 R237 P239 I240 G241 R242 P243 P245 W249 T253 G255 K256 K257 T258 R259 R260 K262 S265 K274

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0016740 transferase activity
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7p48, PDBe:7p48, PDBj:7p48
• PDBsum: 7p48
• PubMed: 35137182
• UniProt: B3VKN3