Structure of PDB 7ohx Chain C Binding Site BS01

Receptor Information

>7ohx Chain C (length=343) Species: 559292 (Saccharomyces cerevisiae S288C)

SRPQVTVHSLTGEATANALPLPAVFSAPIRPDIVHTVFTSVNKNKRQAYA
VSEKAGHQTSAESWGTGRAVARIPRVGGGGTGRSGQGAFGNMCRGGRMFA
PTKTWRKWNVKVNHNEKRYATASAIAATAVASLVLARGHRVEKIPEIPLV
VSTDLESIQKTKEAVAALKAVGAHSDLLKVLKSKKLRAGKGKYRNRRWTQ
RRGPLVVYAEDNGIVKALRNVPGVETANVASLNLLQLAPGAHLGRFVIWT
EAAFTKLDQVWGSETVASSKVGYTLPSHIISTSDVTRIINSSEIQSAIRP
AGQATQKRTHVLKKNPLKNKQVLLRLNPYAKVFAAEKLGSKKA

Ligand information

>7ohx Chain 1 (length=1561)

guuugaccucaaaucagguaggaguacccgcugaauaucaauaagcggag
gaaaagaaaccaaccgggauugccuuaguaacgcgagugaagcggcaaaa
gcucaaauuugaaaucugguaccuucggugcccgaguuguaauuuggaga
gggcacuuuggggccguuccuugucuauguuccuuggaacaggacgucau
agagggugagaaucccguguggcgaggagugcgguucuuuguaaagugcc
uucgaagagucgaguuguuuggaugcagcucuaagugggugguuccaucu
aaagcuaaauauuggcgagagaccgauagcgaacaguacagugauggaaa
gaugaaaagaacuuugaaaagagagugaaaaaguacgugaaauuguugaa
aggaagggcauuugaucagacaugguguuuugugcccucugcuccuugug
gguaggggaucucgcauuucacugggccagcaucaguuuugguggcagga
uaaauccauaggaauguagcuugccgguaaguauuauagccugugggaau
acugccagcugggacugaggacugcgacguaagucaaggaugcuggcaua
augguuauaugccgcccgucuugaaacacggaccaaggagucuaacgucu
augcgaguguuuggguguaaaacccauacgcguaauugaacguagguugg
ggccucgcaagaggugcacaaucgaccgauccuggauggauuugguaaga
gcauagcuguugggacccgaaagauggugaaaaccaucuaguacugguuc
cugccgaaguuucccucaggauagcagaagcucguaucaguuuuauguaa
gaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgaugc
gggaugaaccgaacguagaguuaaggugccggaauacacgcucaagaaca
aaauaguucaucuagaccggguggccauggaagucggaauccgcuaagga
guguguaacaaccggaugaacuagcccugggcgcucaagcguguuaccua
uacucuaccgucaggguugauaugaugcccugacgaguaggcaggcgugg
aggucagugacgaagccuagaccguaaggucgggucgaacggccucuagu
gcagaucuuggugguaguagcagcgaaaccacaccaaggggcuuggcuac
cgaagcagaaguaauguuaccgcaauaguaauugaacuuaguacgagagg
aacaguucauucggauaauugguuuuugcggcugucucaggcagccgcga
agcuaccauccgcuggauuauggcugaacccucuaagucagauccaugcu
agaagcggugaugcuccacacagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugcguggggauaaaucauuuguauac
gacuuagauguacaacgggguauuguaagcgcugagauuaagccuuuguu
gucugauuugu

..........................<<<<<<...........>>>>>.>
.........<<....>>..<<<<<......<<....>>.....>>>>>..
<<<...........<<.<<<<<<...>>>>>>.>>.............<<
<<<<<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>>>.((...
...<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....>>>>>>>>
>>>........<<<<<<<......>>>>>>>.....<<<<<<..>>>>>>
..>>>.................................<<....>>....
...........<<<......>>>...........................
......<<<<<.<<<<<<.....<<<<...<<<..<<<<<<<<<.....>
>>>>>>>>.....>>>...>>>>..<<<<<<<.<<<<<<<<<<<<<<<..
...<<<<<<<<<......<<<<<....>>>>>.......>>>>>>>.>.>
.>>>>>.>>>>>>>>>>........<<<....>>>.....>>>>>>>...
.>>>>>>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<
<<<<..<<<...<<<<.....>>>>...>>>.............<<<<<.
.<<<<<....>>>>>...>>>>>.<<<...<<.....>>....>>>....
>>>>>>.>>>>>>..<<.<...<<<<<<....>>>>>>.....>.>>..<
<<<<<......<<......>>..<<<<<<<<<....<<<.<<<<<..>>>
.>>>>>..>>>>>>>.>>..<<<<.<.<<<.....>>>>..>>>>....<
<<......>>>...<<<<<<<..<<<<(((.....<<<<<<....<....
...<<<<<<<<......<<.....<<.........>>.............
..<<......>>>>.>>>>>>>>......))).>....>>>>>>.>>>>.
.>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<
<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..
>.....>>>>>><<.....<<.>>....>>...<<<<<...>>>>>.<<<
..........>>>..<<<<<<<<..<<<<.<.<<<<<<............
...>>>>>>.><<<<<...<<<<....<<<<<...........>>>>>..
.>>>>..>>>>>.<<<<...<<<<<..........>>>>>..>>>>.>>>
>...>>>>>>>>.<<<<<<......<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>............>>>>>>.<<<<<<<<........
.....>.....<<<<<<<<<.<<<....<..>...>>>....>>>.>>>>
>>.>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohx Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): R31 I34 H36 K44 N45 K46 R47 Q48 Y50 V52 S53 A56 G57 H58 T60 S61 V71 A72 R73 I74 P75 R76 V77 G80 G81 T82 R84 Q87 N92 M93 R95 G96 R98 M99 F100 A101 T103 K104 R107 K108 V111 K112 N114 H115 N116 E117 K118 R119 R138 R141 K161 T162 K163 L179 K180 K183 S184 K186 R188 A189 G190 K191 K193 Y194 R195 N196 R197 R202 R203 Y209 K217 A218 R220 N221 T227 S232 N234 P240 G241 H243 V272 T287 I290 N291 S293 Q296 R300 A302 A305 Q307 K308 R309 H311 V312 L313 K314 K315 N316 N320 K321 Q322 L324 R326 A331 K332 F334 S341 K342 K343
• Binding residue (residue number reindexed from 1): R30 I33 H35 K43 N44 K45 R46 Q47 Y49 V51 S52 A55 G56 H57 T59 S60 V70 A71 R72 I73 P74 R75 V76 G79 G80 T81 R83 Q86 N91 M92 R94 G95 R97 M98 F99 A100 T102 K103 R106 K107 V110 K111 N113 H114 N115 E116 K117 R118 R137 R140 K160 T161 K162 L178 K179 K182 S183 K185 R187 A188 G189 K190 K192 Y193 R194 N195 R196 R201 R202 Y208 K216 A217 R219 N220 T226 S231 N233 P239 G240 H242 V271 T286 I289 N290 S292 Q295 R299 A301 A304 Q306 K307 R308 H310 V311 L312 K313 K314 N315 N319 K320 Q321 L323 R325 A330 K331 F333 S340 K341 K342

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohx, PDBe:7ohx, PDBj:7ohx
• PDBsum: 7ohx
• PubMed: 34813592
• UniProt: P10664|RL4A_YEAST Large ribosomal subunit protein uL4A (Gene Name=RPL4A)