Structure of PDB 7ohu Chain C Binding Site BS01

Receptor Information

>7ohu Chain C (length=336) Species: 559292 (Saccharomyces cerevisiae S288C)

SRPQVTVHSLTGEATANALPLPAVFSAPIRPDIVHTVFTSVNKNKRQAYA
VSEKAGHQTSAESWVARIPRVGGTGRSGQGAFGNMCRGGRMFAPTKTWRK
WNVKVNHNEKRYATASAIAATAVASLVLARGHRVEKIPEIPLVVSTDLES
IQKTKEAVAALKAVGAHSDLLKVLKSKKLRAGKGKYRNRRWTQRRGPLVV
YAEDNGIVKALRNVPGVETANVASLNLLQLAPGAHLGRFVIWTEAAFTKL
DQVWGSETVASSKVGYTLPSHIISTSDVTRIINSSEIQSAIRPAGQATQK
RTHVLKKNPLKNKQVLLRLNPYAKVFAAEKLGSKKA

Ligand information

>7ohu Chain 1 (length=1692)

guuugaccucaaaucagguaggaguacccgcugaacuuauaucaauaagc
ggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagcgg
caaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaauuu
ggagagggcacuuuggggccguuccuugucuauguuccuuggaacaggac
gucauagagggugagaaucccguguggcgaggagugcgguucuuuguaaa
gugccuucgaagagucgaguuguuugggaaugcagcucuaaguggguggu
uccaucuaaagcuaaauauuggcgagagaccgauagcgaacaaguacagu
gauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacgugaaa
uuguugaaagggaagggcauuugaucagacaugguguuuuguggggaauc
ucgcauuucacugggccagcaucaguuuugguggcaggauaaauccauag
gaauguagcuugccucgguaaguauuauagccugugggaauacugccagc
ugggacugaggacugcgacguaagucaaggaugcuggcauaaugguuaua
ugccgccgucuugaaacacggaccaagggucuaacgucuaugcgaguguu
uggguguaaaacccauacgcguaaugaaagugaacguagguuggggccuc
gcaagaggugcacaaucgaccgauccugaggauggauuugaguaagagca
uagcuguugggacccgaaagauggugaaaccaucuaguacugguuccugc
cgaaguuucccucaggauagcagaagcucguaucaguuuuauuaaaauga
agagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggaug
aaccgaacguagaguuaaggugccggaauacacgcucaucagaccaaaag
guguuaguucaucuagacagccggacgguggccauggaagucggaauccg
cuaaggaguguguaacaacucaccggccgaaugaacuagcccugaauggc
gcucaagcguguuaccuauacucuaccgucaggguugauaugaugcccug
acgaguaggcaggcguggaggucagugacgaagccuagaccguaaggucg
ggucgaacggccucuagugagaucuuggugguaguagcagcgaaaccaca
gccaagggacgggcuuggcagaaucagcgggggcuuguggcagucaagcg
gcgacauugcucauaccgaagcagaauucgguaagcgucuacugaugaug
uuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggau
aauugguuuuugcggcugucugaucaggcagccgcgaagcuaccauccgc
uggauuauggcugaacgccucuaagucagauccaugcuagaacgcgguga
uuucuuugcuccacacaauauagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucau
uuguauacgacuuagauguacaacgggguauuguaagcaguagaguagcc
ugguuacgaucugcugagauuaaccuuuguugucugauuugu

..........................<<<<<<...............>>>
>>.>.........<<....>>..<<<<<......<<.....>>.....>>
>>>..<<<..<........<<.<<<<<<...>>>>>>.>>........>.
...<<<<<<<<<<..<<<<<<<<<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>>..>>>>>>>......>>
>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<<<.
.>>>>>>..>>>..................................<<..
..>>...............<<<<....>>>>...................
...............<<<<<.<<<<<.<....<<<<...<<<........
..>>>...>>>>..<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<
<......<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>
>>>>>>>>........<<<....>>>.....>>>>>>>....>>>>>>.>
>>>>.<<<<........>>>><<<<<<...<<<<<<.<<<<<<..<<<..
.<<<<.....>>>>...>>>..................<<<<<..<<<<<
....>>>>>...>>>>>.<<<<<<<<.......>>>.>>>.>>....>>>
>>>.>>>>>>..<<.<...<<<<<<...>>>>>>.....>.>>..<<<<<
<....<.<<......>>.><<<<<<<<<....<<<.<<<<...>>>>>>>
..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<....
..>>>...<<<<<<<..<<<<(((.....<<<<<<......<......<<
<.<<<<<<<<<<.......<<<<<.<<....<<.........>>....>>
.........<<......>>...>>>>>...>>>>>>>>>>>>>....)))
.>....>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>
>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>....>>>>>><<.....<<.>>....>>...
.<<<<<.......>>>>>....<<<<<..<.........<<<<....<<.
>>....>>>>...<<<<<<<......>>>>>>>......>.>>>>>...<
<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<
...<<......<<<<<<<<........>>>>>>>>.....>>..>>>>>.
<<<<...<<<<<...........>>>>>..>>>>.>>>>....>>>>>>>
>.......<<<<<<.<<<.<<....<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<
.............>.....<<<<<<<<<.<<<....<<<<....<<.<<.
..>>.>>...>>>>...>>>...>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohu Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): R31 I34 H36 T40 V42 K44 N45 K46 R47 Q48 Y50 V52 S53 K55 A56 H58 Q59 T60 S61 V71 A72 R73 I74 P75 G81 T82 R84 N92 M93 R95 G96 R98 M99 F100 A101 P102 R107 K108 V111 K112 V113 N114 H115 N116 K118 R119 Y120 R138 R141 K161 T162 K163 L179 K180 S184 K186 L187 R188 G190 K191 G192 K193 Y194 R195 N196 R197 W199 R202 R203 Y209 K217 R220 N221 P223 T227 S232 N234 P240 G241 H243 V272 T287 N291 Q296 R300 A305 Q307 K308 R309 T310 H311 V312 K315 N316 K319 N320 K321 Q322 L324 R326 N328 A331 K332 F334 S341 K342 K343 A344
• Binding residue (residue number reindexed from 1): R30 I33 H35 T39 V41 K43 N44 K45 R46 Q47 Y49 V51 S52 K54 A55 H57 Q58 T59 S60 V65 A66 R67 I68 P69 G73 T74 R76 N84 M85 R87 G88 R90 M91 F92 A93 P94 R99 K100 V103 K104 V105 N106 H107 N108 K110 R111 Y112 R130 R133 K153 T154 K155 L171 K172 S176 K178 L179 R180 G182 K183 G184 K185 Y186 R187 N188 R189 W191 R194 R195 Y201 K209 R212 N213 P215 T219 S224 N226 P232 G233 H235 V264 T279 N283 Q288 R292 A297 Q299 K300 R301 T302 H303 V304 K307 N308 K311 N312 K313 Q314 L316 R318 N320 A323 K324 F326 S333 K334 K335 A336

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohu, PDBe:7ohu, PDBj:7ohu
• PDBsum: 7ohu
• PubMed: 34813592
• UniProt: P10664|RL4A_YEAST Large ribosomal subunit protein uL4A (Gene Name=RPL4A)