Structure of PDB 7mi4 Chain C Binding Site BS01

Receptor Information

>7mi4 Chain C (length=554) Species: 243231 (Geobacter sulfurreducens PCA)

DGSIPLIPVRMLNEHVYCPRLAYLMWVQGEFSHNEFTVDGVIRHRRVDAG
GGVLPSETQEDSRIHARSVSLSSERLGITAKIDLVEGEGAYVSPVDYKRG
KRPHVAGGAYEPERVQLCAQGLLLREHGFASDGGALYFVASRERVPVAFD
DELIGRTLAAIDEMGRTALSGTMPPPLEDSPKCPRCSLVGICLPDEVRFL
SHLSVEPRPIIPADGRGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARL
GETSQVALFGNATLTTAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNV
ETRTYQYQRSFDPETCLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPP
GLLMSLQDDMRHAMRAPSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMG
FDFTTRNRRPPKDPVNALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPR
FGRPALALDMMEPFRPLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSA
RKRFIAGFERRMEQEVTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYP
NFVT

Ligand information

>7mi4 Chain G (length=35)

gtcgtagctgaggcctcagctacgactttttgaat

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mi4 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): P12 R14 E18 Y21 M29 F35 T41 H48 A84 K85 R103 Y114 P116 K186 R189 C190 S191 R278
• Binding residue (residue number reindexed from 1): P8 R10 E14 Y17 M25 F31 T37 H44 A80 K81 R99 Y110 P112 K182 R185 C186 S187 R274

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mi4, PDBe:7mi4, PDBj:7mi4
• PDBsum: 7mi4
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)