Structure of PDB 7bt6 Chain C Binding Site BS01

Receptor Information

>7bt6 Chain C (length=334) Species: 559292 (Saccharomyces cerevisiae S288C)

PQVTVHSLTGEATANALPLPAVFSAPIRPDIVHTVFTSVNKNKRQAYAVS
EKAGHQTSAGAFGNMCRGGRMFAPTKTWRKWNVKVNHNEKRYATASAIAA
TAVASLVLARGHRVEKIPEIPLVVSTDLESIQKTKEAVAALKAVGAHSDL
LKVLKSKKLRAGKGKYRNRRWTQRRGPLVVYAEDNGIVKALRNVPGVETA
NVASLNLLQLAPGAHLGRFVIWTEAAFTKLDQVWGSETVASSKVGYTLPS
HIISTSDVTRIINSSEIQSAIRPAGQATQKRTHVLKKNPLKNKQVLLRLN
PYAKVFAAEKLGSKKAEKTGTKPAAVFTETLKHD

Ligand information

>7bt6 Chain 1 (length=2962)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaggccagcauc
aguuuugguggcaggauaaauccauaggaauguagcuugccucgguaagu
auuauagccugugggaauacugccagcugggacugaggacugcgacguaa
gucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacacgga
ccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcg
uaaugaaagugaacguagguuggggccucgcaagaggugcacaaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
aacuuuaaauauguaagaaguccuuguuugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguagaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacucuaccgucaggguugauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuagacc
guaaggucgggucgaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uuccaucgaaagggaauccgguuaagauuccggaaccuggauauggauuc
uucacgguaacguaacugaauguggagacgucggcgcgagcccugggagg
aguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccgg
agauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggug
cgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcca
ggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguug
auagaauaauguagauaagggaagucggcaaaauagauccguaacuucgg
gauaaggauuggcucuaagggucggacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacucuc
uuaagguagccaaauggauuaacgagauucccacugucccuaucuacuau
cuagcgaaaccacagccaagggggcuuggcagaaucagcggggaaagaag
acccuguugagcuugacucuaguuugacauugugaagagacauaguuucu
uuacuuauucaagacauugucagguggggaguuugggggcggcacaucug
uuaaacgauaacgcagauguccuaaggggggcucauggagaacagaaauc
uccaguagaacaaaaggguaaaagcccccuugauuuugauuuucagugug
aauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaauu
ugaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggc
agucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucu
uccuaucauaccgaagcagaauucgguaagcguuggauuguucacccacu
aauagggagugagcuggguuuagaccgucgugagacagguuaguuuuacc
cuacugaugaauguuaccgcaauaguaauugaacuuaguacgagaggaac
aguucauucggauaauugguuuuugcggcugucugaucaggcauugccgc
gaagcuaccauccgcuggauuauggcugaacgccucuaagucagaaucca
ugcuagaacgcggugauuucuuugcuccacacaauauagauggauacgaa
uaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugca
cuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuuug
cguggggauaaaucauuuguauacgacuuagauguacaacgggguauugu
aagcaguagaguagccuuguuguuacgaucugcugagauuaagccuuugu
ugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>......
.>>....<<<<<..<<<<<..<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>....
..>>>>>.>>>>>........<<<<<<<.<....>.>>>>>>>.....((
(((..<<<<<<)))))..]]].............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<<.....<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>
.......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....
>>>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><
<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.
..<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<
<<<<<....<....>....>>>>.>>.>>....>>>>>>.>>>>>>..<<
.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>
>>...<<.......>>......>>>>>>......>.>>..<<<<<<....
<.<<<....>>>.><<.<<.<......<<<.<<<<<<<............
...........>>>>>...<<<<..<<....>>..>>>>.....>>>>>.
....>.>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<<....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
.....>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<..
...<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<
<<..{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>.
...>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>..
.>>>>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>
>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<.....<<<<<<<.<<.........<.....<<<<<....<<..>>
.....>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>...............<<.............<
<<<....>>>>..........>>...........<<.........>>...
..........<<..>>......>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<....>>>>>....[[[[[..{{{.......
.((((((..[[[[..<<<<<..<<.<.<<..<<<<<.............>
>>>>...<...>.....>>.>.>>...>>>>>......<.<..<<<<<<<
<...........>>>>>>>>.....<<<<<<<...<<<<<<.......>>
>>>>.....<<......>>....>>>>>>>...<<<.<<<<<.......>
>>>.>>>>.<<<....>>>...>.>...[...(((((...((((.>>>>>
>)))).)))))....].....]]]]...))))))..............<<
<<<...<<.......>>...>>>>>.................<<<<<...
<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>..
...>>>>.....>>>>>.....<<<<<<<....>>>.>>>>........}
}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<...............
>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>..>>>>>
...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>
.>>>>....>>>>>>>>.......<<<<<<.<<<.<.....<<.......
...<<<<<<.....>>>>>>......>>.......<<<<<<...<<<<<.
<..<<<......>>>..>.>>>>>.>>>>>>..............>.>>>
.>>>>>>..<<<<<<<.............>.....<<<<<<<<<.<<<..
..<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>>
>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bt6 Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel.
• Resolution: 3.12 Å
• Binding residue (original residue number in PDB): R31 H36 K44 N45 K46 R47 Q48 Y50 V52 S53 A56 N92 M93 R95 R98 F100 K108 K112 N114 H115 N116 E117 K118 R119 R138 R141 K161 T162 K180 K186 R188 A189 G190 K191 K193 Y194 R195 R197 R202 R203 Y209 K217 R220 N221 T227 N234 P240 G241 H243 N291 Q296 R300 A302 G303 A305 Q307 K308 R309 V312 K315 Q322 R326 N328 K332 F334 A335 K343 T347 P351
• Binding residue (residue number reindexed from 1): R28 H33 K41 N42 K43 R44 Q45 Y47 V49 S50 A53 N64 M65 R67 R70 F72 K80 K84 N86 H87 N88 E89 K90 R91 R110 R113 K133 T134 K152 K158 R160 A161 G162 K163 K165 Y166 R167 R169 R174 R175 Y181 K189 R192 N193 T199 N206 P212 G213 H215 N263 Q268 R272 A274 G275 A277 Q279 K280 R281 V284 K287 Q294 R298 N300 K304 F306 A307 K315 T319 P323

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bt6, PDBe:7bt6, PDBj:7bt6
• PDBsum: 7bt6
• PubMed: 33037216
• UniProt: P10664|RL4A_YEAST Large ribosomal subunit protein uL4A (Gene Name=RPL4A)