Structure of PDB 7asm Chain C Binding Site BS01

Receptor Information

>7asm Chain C (length=274) Species: 1280 (Staphylococcus aureus)

AIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRNNQGKLTV
RHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEK
RYIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKG
GQIARSAGASAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHE
LVNVGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWG
KPTLGKKTRRGKKSSDKLIVRGRK

Ligand information

>7asm Chain A (length=2834)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuacggaguuacaaaggacgacauuagacgaaucaucuggaaa
gaugaaucaaagaagguaauaauccuguagucgaaaauguugucucucuu
gaguggauccugaguacgacggagcacgugaaauuccgucggaaucuggg
aggaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccag
uaccgugagggaaaggugaaaagcaccccggaaggggagugaaauagaac
cugaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcg
ugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagc
aguaaauguggagccguagcgaaagcgagucugaauagggcguuuaguau
uuggucguagacccgaaaccaggugaucuacccuuggucagguugaaguu
caggcugaauggaggaccgaaccgacuuacguugaaaagugagcggauga
acugaggguagcggagaaauuccaaucgaaccuggagauagcugguucuc
uccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagag
cacuguuuggacgaggggccgguuaccgaauucagacaaacuccgaaugc
caauuaauuuaacuugggagucagaacaugggugauaagguccguguucg
aaagggaaacagcccagaccaccagcuaaggucccaaaauauauguuaag
uggaaaaggauguggcguugcccagacaacgucgagugacacugcgccga
aaauguaccggggcuaaacauauuaccgaagcuguggauuguccuuugga
caaugguaggagagcguucuaagggcguugaagcaugaucguaaggacau
guggagcgcuuagaagugagaaugccggugugaguagcgaaagacgggug
agaaucccguccaccgauugacuaagguuuccagaggaaggcucguccgc
ucuggguuagucggguccuaagcugaggccgacaggcguaggcgauggau
aacagguugauauuccuguaccaccuauaaucguuuuaaucgaugggggg
acgcaguaggauaggcgaagcgugcgauuggauugcacgucuaagcagua
aggcugaguauugguacucguuaaggcugagcugugauggggagaagaca
uugugucuucgagucguugauuucacacugccgagaaaagccucuagaua
gaaaauaggugcccguaccgcaaaccgacacagguagucaagaugagaau
ucuaaggugagcgagcgaacucucguuaaggaacucggcaaaaugacccc
guaacuucgggagaaggggugcucuuuaggguuaacgcccagaagagccg
cagugaauaggcccaagcgacuguuuaucaaaaacacaggucucugcuaa
accguaaggugauguauaggggcugacgccugcccggugcuggaagguua
agaggagugguuagcuucugcgaagcuacgaaucgaagccccaguaaacg
gcggccguauaacgguccuaagguagcgaaauuccuugucggguaaguuc
cgacccgcacgaaaggcguaacgauuugggcacugucucaacgagagacu
cggugaaaucauaguaccugugaagaugcagguuacccgcgacaggacgg
aaagaccccguggagcuuuacuguagccugauauugaaauucggcacgcu
uguacaggauagguaggagccuuugaaacgugagcgcuagcuuacgugga
ggcgcuggugggauacuacccagcuguguuggcuuucuaacccgcaccac
uuaucguggugggagacagugucaggcgggcaguuugacuggggcggucg
ccuccuaaaagguaacggaggcgcucaaagguucccucagaaugguugga
aaucauucauagaguguaaaggcauaagggagcuugacugcgagaccuac
aagucgagcagggucgaaagacggacuuagugauccggugguuccgcaug
gaagggccaucgcucaacggauaaaagcuaccccggggauaacaggcuua
ucucccccaagaguucacaucgacggggagguuuggcaccucgaugucgg
cucaucgcauccuggggcuguagucggucccaaggguugggcuguucgcc
cauuaaagcgguacgcgagcuggguucagaacgucgugagacaguucggu
cccuauccgucgugggcguaggaaauuugagaggagcuguccuuaguacg
agaggaccgggauggacauaccucugguguaccaguugucgugccaacgg
cauagcuggguagcuauguguggacgggauaagugcugaaagcaucuaag
caugaagccccccucaagaugagauuucccaacuucgguuauaagauccc
ucaaagaugaugagguuaauagguucgagguggaagcauggugacaugug
gagcugacgaauacuaaucgaucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[.<.....
.<<<<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>.....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<...<<<<.......<<<<<<<<<<.<<<...<<<<<......
>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>.
>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<<<
<<..<<<<>>>>>..)))......]].>>>>>>>.]].............
..<<....>>....]].........<<<<....>>>>.............
.....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>.
..<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<
<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>
>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<
<<<.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....
>>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<
<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<
...<<<<<<<<...<<.........>>...>>>>>>>>..>>.>.....>
>>>>.>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>.
...<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<....
.......<<..<<<<.<<<<........<..>....>>>>.>>>>.>>..
.........))).....>>>>>>.>>>...>>>>>>><<<<<<<...>>>
>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<....>>.>>>
>>...>>.>>>>>>>..>.....>>>>>><......<<....<<<<<<..
.....>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>
>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>..
.<<<<<........>>>>>))<<<<<<<..........<<<...<<<<..
..<<<<...<<...<....<.<<<...{.{...>>>.>.<....<<<<..
.<<<.<<<<<<..>>>>>>......>>>...>>>>.....>.........
.................>....>>...>>>>.........>>>>..>>>.
....>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<.......
>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<
....<<..>>.....>>>>><<<<<<..<.........>...>>>>>>..
..........<<<<<<<<<..<<<.........>>>..<<<<<<<.....
<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<..
.<<...<<<<<<...........>>>>>>...>>...>>>>>>>>..>>>
>><<<<<.....>>>>>........<...........<<<<<<.....>>
>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>
>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.(((.[[
......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<.<<<<<<<<<.
.............<<<..<<.........<<<<<<....>>>>>>.....
.>>............>>>....>.>>>>>>>>.>>>>....<<.<<<<<<
.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><
<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.....
..>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<....
..>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>>
>..))>>>>>>>.............>>>>..]]>>>>.......<<<<.<
.<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<<<
<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>
>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>...
.]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<.......
........>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}.}>>>>
>>..>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>
>>>....>>>.>>>>.......>>>>>>>.<<<....>>>....<<<<<<
<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>.
..>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7asm Staphylococcus aureus 50S after 30 minutes incubation a 37C
• Resolution: 2.48 Å
• Binding residue (original residue number in PDB): P8 I9 T10 N11 R14 K35 K39 A41 G42 R43 N44 N45 Q46 K48 L49 T50 R52 H54 H58 K59 R60 Y62 R63 Y83 R87 D98 G99 K147 G149 K150 Q153 I154 A155 R156 S157 A158 K170 Y171 L176 R177 S178 E180 R182 H200 E201 N204 V205 G206 K207 A208 G209 R210 R212 W213 R217 P218 T219 V220 R221 S223 V224 M225 N226 N228 D229 H232 G234 G235 E236 G237 R238 P240 G242 R243 P249 W250 T254 L255 G256 K257 K258 T259 R260 R261 S266 K268 R274
• Binding residue (residue number reindexed from 1): P7 I8 T9 N10 R13 K34 K38 A40 G41 R42 N43 N44 Q45 K47 L48 T49 R51 H53 H57 K58 R59 Y61 R62 Y82 R86 D97 G98 K146 G148 K149 Q152 I153 A154 R155 S156 A157 K169 Y170 L175 R176 S177 E179 R181 H199 E200 N203 V204 G205 K206 A207 G208 R209 R211 W212 R216 P217 T218 V219 R220 S222 V223 M224 N225 N227 D228 H231 G233 G234 E235 G236 R237 P239 G241 R242 P248 W249 T253 L254 G255 K256 K257 T258 R259 R260 S265 K267 R273

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0016740 transferase activity
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7asm, PDBe:7asm, PDBj:7asm
• PDBsum: 7asm
• UniProt: P60430|RL2_STAA8 Large ribosomal subunit protein uL2 (Gene Name=rplB)