Structure of PDB 5x8t Chain C Binding Site BS01

Receptor Information

>5x8t Chain C (length=247) Species: 3562 (Spinacia oleracea)

NPRNNLISGQRRCGKGRNARGIITARHRGGGHKRLYRKIDFRRNEKDIYG
KIVTIEYDPNRNAYICLIHYGDGEKRYILHPRGAIIGDTIVSGTEVPIKM
GNALPLTDMPLGTAIHNIEITLGRGGQLARAAGAVAKLIAKEGKSATLKL
PSGEVRLISKNCSATVGQVGNVGVNQKRLGRAGSKRWLGKRPVVRGVVMN
PVDHPHGGGEGRAPIGRKSPTTPWGYPALGRRSRKRNKYSDNFIIRR

Ligand information

>5x8t Chain A (length=2809)

ucaaacgaggaaaggcuuacgguggauaccuaggcacccagagacgagga
agggcguauuaaucgacgaaaugcuucggggaguugaaaauaagcagaga
uccggagauucccgaauaggucaaccuuucgaacuucugcugaauccaug
ggcaggcaagagacaaccuggcgaacugaaacaucuuaguagccagagga
aaagaaagcaaaagcgauucccguaguagcggcgagcgaaaugggagcag
ccuaaaccgugaaaacgggguugugggagagcaauacaagcgucgugcug
cuaggcgaaucaguggagugcggaacccuagauggugaaaguccaguagc
cgaaagcaucacuagcuuaugcucugacccgaguagcauggggcacgugg
aaucccgugugaaucagcaaggaccaccuugcaaggcuaaauacuccugg
gugaccgauagcgaaguaguaccgugagggaagggugaaaagaaccccca
ucggggagugaaauagaacaugaaaccguaagcucucaagcagugggagg
gggaccagacccugaccgcgugccuguugaagaaugagccggcgacucau
aggcaguggcuugguuaagggaacccaccggagccguagcgaaagcgagu
cuucauagggcaauugucacugcuuauggacccgaaccugggugaucuau
ccaugaccaggaugaagcuugggugaaacuaaguggagguccgaaccgac
ugauguugaagaaucagcggaugaguugugguuaggggugaaaugccacu
cgaacccagagcuagcugguucuccccgaaaugcguugaggcgcagcagu
ugacuggacaucuagggguaaagcacuguuucggugcgggccgcgagagc
gguaccaaaucgaggcaaacucugaauacuagauaugaccuccaaauaac
aggggucaaggucggccagugagacgaugggggauaagcuucaucgucga
gagggaaacagcccggaucaccagcuaaggccccuaaaugaccgcucagu
gauaaaggagguaggggugcagagacagccaggagguuugccuagaagca
gccacccuugaaagagugcguaauagcucacugaucgagcgcucuugcgc
cgaagaugaacggggcuaagcggucugccgaagcugugggauguaaaaaa
acaucgguaggggagcguuccguguuagggagaaacgcgugcgugagccg
cguuggacgaagcggaagcgagaaugucggcuugaguaacgcaaacauug
gugagaauccaaugccccgaaaaccuaaggguuccuccgcaagguucguc
cacggagggugagucagggccuaagaucaggccgaaaggcguagucgaug
gacaacaggugaauauuccuguacuaccccuuguuggucccgagggacgg
aggaggcuagguuagccgaaagaugguuaucgguucaaggacgcaaggug
acccuguuuuucaggguaagaagggguagagaaaaugccucgagccaaug
uucgaguaccaggcgcuacggcgcugaaguaaccgaugccauacucccag
gaaaagcucgaacgaccuucaacaaaaggguaccuguacccgaaaccgac
acagguagguagguagagaauaccuaggggcgcgagacaacucucucuaa
ggaacucggcaaaauagccccguaacuucgggagaaggggugcccccuca
caaagggggucgaagugaccaggcccgggcgacuguuuaccaaaaacaca
ggucuccgcaaagucguaagaccauguaugggggcugacgccugcccagu
gccggaaggucaaggaaguuggugaccugaugacaggggagccggcgacc
gaagccccggugaacggcggccguaacuauaacgguccuaagguagcgaa
auuccuugucggguaaguuccgacccgcacgaaaggcguaacgaucuggg
cacugucucggagagaggcucggugaaauagacaugucugugaagaugcg
gacuaccugcaccuggacagaaagacccuaugaagcuuuacuguucccug
ggauuggcuuugggcuuuuccugcgcagcuuagguggaaggcgaagaagg
cccccuuccgggggggcccgagccaucagugagauaccacucuggaagag
cuagaauucuaaccuugugucaggaccuacgggccaagggacauucucag
guagacaguuucuauggggcguaggccucccaaaagguaacggaggcgug
caaagguuuccucgggccggacggagauuggcccucgagugcaaaggcag
aagggagcuugacugcaagacccacccgucgagcagggacgaaagucggc
cuuagugauccgacggugccgaguggaagggccgucgcucaacggauaaa
aguuacucuagggauaacaggcugaucuuccccaagaguucacaucgacg
ggaagguuuggcaccucgaugucggcucuucgccaccuggggcuguagua
uguuccaaggguugggcuguucgcccauuaaagcgguacgugagcugggu
ucagaacgucgugagacaguucgguccauauccggugugggcguuagagc
auugagaggaccuuucccuaguacgagaggaccgggaaggacgcaccucu
gguguaccaguuaucgugcccacgguaaacgcuggguagccaagugcgga
gcggauaacugcugaaagcaucuaaguaguaagcccaccccaagaugagu
gcucuccua

.............<<<<<<<<<<.....<<..<<<<<<<<<<<.<.....
.<<<<<<.....((.>>>.....<<<<<<<..<<.))......>>.....
>>>>>>>...>>>....<<<....>>>......<<<<<<<<.........
>>>>>..>>>.>....<<<<<<.....<<....>>......>>>>>.>..
.......<<....>>..<<<<<<.....<<.....>>....>>>>>>..<
<<....<<<<....>>>>......<<<<<<<<<....<<<<...<<<.<<
<<.<<<...<<.<<.....>>.>>((......<<<.....))>>>...>>
>...>>>>.>>>..>>>>.>>>>>>..>>>.....<<<<<<<<.......
..>>>>>>>>......<<<<<..<<<<>>>>>..>>>......>>.>>>>
>>>.>>...............<<....>>....>>.........<<<<..
..>>>>..................>>>>>>>>>>........<<<<..<<
<<.......>>>>..>>>>...<.<........>.>.<<<<<<....<<<
<<<<<<<<<...<<<...<<.......>>...>>>...<<....>>..<<
<<.....>>>>.....>>>>>>>>>>>>..<<.<...<<<<<<.....<.
<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<.<
<<<<........>>>>>>>>....>>>>>>>.>...<<<.....>>>...
...>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<.<<
<<<<<....<<<<<<.....<<<...<<<<<<<<...<<<<<........
>>>.>>...>>>>>>>>..>>.>.....>>>>>>..<<<<<<........
.>>>>>>..>>>>>>>..>>..<<<<<<<<<<......>>>>>>>>>>..
..<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.....
......<<..<<<<<<<<<<.........<<<.<<....<<.........
>>....>>.........<<......>>...>>>.....>>>>>>>>>>.>
>..........))).....>>>>>>.>>>...>>>>>>><<<<<......
>>>>>....>>>>>><.<<<<<<...........<<<<<.<<....>>>>
>>.>.......>>>>>>..>.....>>>>>><......<<.....<<<<<
<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...>
>...<<<<<........>>>>>))<<<<<.<<<<.<<<<.<<<<....<<
<<..<<<..<<<<<<<<<<.....(((..>>>>>....<<<<<...<<<.
<<<<<<.....>>>>>>.....<<<<<<.......>>>>>>..>>>..>>
>>>.....<..<<<<<....>>>>>...>>>>>>...>>>...>>>>...
......>>>>...>>>>....>>>>..>>>>>>>>>..>>.....<<...
...>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<
<.........<.....<<<<<....<....>.....>>>>><<<<<<<..
...>>>>>>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<
<<<<<..<<<...<<..<<<<<<..<<<<....>>>>...>>>>>>..>>
...>>>>>>>>..>>>>><<<<<<.......>>>>>>........<....
.......<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>
>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>
>>>..<<<<<<<<.<<<..<......<<<<.<<<<<<..<<<<<..<<<<
<<<..<<.<<<.<<<<<<<<..............<<<..<<<......<<
<<<<.......>.>>>>>...>>>............>>>.....>>>>>>
>>.>>>.>>...<<<<<.<<<..........>>>>>>>>.....>>>>>>
>...>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<
<...<<<<<<<..<<<<<<<.......>>>>>>>....<<...(((>>..
..>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>
))).......<<<<<<<.<<..>>>>..>>>>>>>>>.............
>>>>..>>>>>>.......<<<<.<.<<<<<<...<<.......>>...>
>>>>>...>>>>.>.......<<<<<...<<<<..<<<<<<<<.......
.>>>>>..>>>.<<<<<.....>>>>>.....>>>>.......>>>>>..
...<<<<<<<....>>>.>>>>....>...>>>>>>>>>>>....<<<<<
<<...<.<<..<<<<<<<...............>>>>>>>.<<<<<...<
<<<...<<<<<<<<<<<))).>>>>>>...>>>>>...>>>>.>>>>>..
<.<<...<<<<<<...........>>>>>>...>>>.>>.>.......>>
>>>>>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5x8t Unique localization of the plastid-specific ribosomal proteins in the chloroplast ribosome small subunit provides mechanistic insights into the chloroplastic translation
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): R25 N27 G31 Q32 R34 C35 K37 G38 R39 A41 R42 G43 I44 I45 T46 A47 H49 R50 G51 G52 H54 K55 L57 Y58 R59 R64 N66 Y79 P81 R83 N84 H91 D94 G95 I142 L144 R146 Q149 L150 A151 R152 A153 A154 G155 L172 P173 S174 R178 V194 N197 K199 R200 L201 R203 A204 G205 K207 R208 W209 K212 R213 P214 V215 V216 R217 G218 V219 V220 M221 P223 V224 D225 H226 E232 G233 R234 P236 I237 G238 R239 K240 P242 T243 P245 W246 Y248 L251 G252 R253 R254 S255 R256 R258 Y261 R269
• Binding residue (residue number reindexed from 1): R3 N5 G9 Q10 R12 C13 K15 G16 R17 A19 R20 G21 I22 I23 T24 A25 H27 R28 G29 G30 H32 K33 L35 Y36 R37 R42 N44 Y57 P59 R61 N62 H69 D72 G73 I120 L122 R124 Q127 L128 A129 R130 A131 A132 G133 L150 P151 S152 R156 V172 N175 K177 R178 L179 R181 A182 G183 K185 R186 W187 K190 R191 P192 V193 V194 R195 G196 V197 V198 M199 P201 V202 D203 H204 E210 G211 R212 P214 I215 G216 R217 K218 P220 T221 P223 W224 Y226 L229 G230 R231 R232 S233 R234 R236 Y239 R247

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0016740 transferase activity
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0009507 chloroplast
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5x8t, PDBe:5x8t, PDBj:5x8t
• PDBsum: 5x8t
• PubMed: 28582576
• UniProt: P06509|RK2_SPIOL Large ribosomal subunit protein uL2cz/uL2cy (Gene Name=rpl2-A)