Structure of PDB 5hr4 Chain C Binding Site BS01

Receptor Information

>5hr4 Chain C (length=749) Species: 17 (Methylophilus methylotrophus)

PQDPINIKAAERMGKLHDTLKLVGYEGHALELYLVRLLFCLFAEDTTIFE
KSLFQEYIETKTLEDGSDLAHHINTLFYVLNTPEQKRLKNLDEHLAAFPY
INGKLFEEPLPPAQFDKAMREALLDLCSLDWSRISPAIFGSLFQSIMDAK
KRRNLGAHYTSEANILKLIKPLFLDELWVEFEKVKNNKNKLLAFHKKLRG
LTFFDPACGCGNFLVITYRELRLLEIEVLRGLHRGGQQVLDIEHLIQINV
DQFFGIEIEEFPAQIAQVALWLTDHQMNMKISDEFGNYFARIPLKSTPHI
LNANALQIDWNDVLEAKKCCFILGNPPFVGKSKQTPGQKADLLSVFGNLK
SASDLDLVAAWYPKAAHYIQTNANIRCAFVSTNSITQGEQVSLLWPLLLS
LGIKINFAHRTFSWTNEASGVAAVHCVIIGFGLKDSDEKIIYEYESINGE
PLAIKAKNINPYLRDGVDVIACKRQQPISKLPSMRYGNKPTDDGNFLFTD
EEKNQFITNEPSSEKYFRRFVGGDEFINNTSRWCLWLDGADISEIRAMPL
VLARIKKVQEFRLKSSAKPTRQSASTPMKFFYISQPDTDYLLIPETSSEN
RQFIPIGFVDRNVISSNATYHIPSAEPLIFGLLSSTMHNCWMRNVGGRLE
SRYRYSASLVYNTFPWIQPNEKQSKAIEEAAFAILKARSNYPNESLAGLY
DPKTMPSELLKAHQKLDKAVDSVYGFKGPNTEIARIAFLFETYQKMTSL

Ligand information

>5hr4 Chain H (length=13)

tatccgacataac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5hr4 Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities.
• Resolution: 2.5964 Å
• Binding residue (original residue number in PDB): R309 G312 H314 N481 F484 G486 K487 S540 G544 E545 W570 A578 A579 V580 K629 Y642 K645 Y738 L805 E806 R810 S814
• Binding residue (residue number reindexed from 1): R153 G156 H158 N325 F328 G330 K331 S384 G388 E389 W414 A422 A423 V424 K473 Y486 K489 Y582 L649 E650 R654 S658

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:5hr4, PDBe:5hr4, PDBj:5hr4
• PDBsum: 5hr4
• PubMed: 27082731
• UniProt: B2MU09