Structure of PDB 5gan Chain C Binding Site BS01

Receptor Information
>5gan Chain C (length=855) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EHTIFTQLKKNIPKTRYNRDYMLSMANIPERIINVGVIGPLHSGKTSLMD
LLVIDSHKRIPDMSKNVELGWKPLRYLDNLKQEIDRGLSIKLNGSTLLCT
DLESKSRMINFLDAPGHVNFMDETAVALAASDLVLIVIDVVEGVTFVVEQ
LIKQSIKNNVAMCFVINKLDRLILDLKLPPMDAYLKLNHIIANINSFTKG
NVFSPIDNNIIFASTKLGFTFTIKEFVSYYYAHSIPSSKIDDFTTRLWGS
VYYHKGNFRTKPFENVEKYPTFVEFILIPLYKIFSYALSMEKDKLKNLLR
SNFRVNLSQEALQYDPQPFLKHVLQLIFRQQTGLVDAITRCYQPFELFDN
KTAHLSIPGKSTPEGTLWAHVLKTVDYGGAEWSLVRIYSGLLKRGDTVRI
LDTSQSESRQKRQLHDISKTETPSCEVEEIGLLGGRYVYPVHEAHKGQIV
LIKGISSAYIKSATLYSVKSKEDMKQLKFFKPLDYITEAVFKIVLQPLLP
RELPKLLDALNKISKYYPGVIIKVEESGEHVILGNGELYMDCLLYDLRAS
YAKIEIKISDPLTVFSESCSNESFASIPVSNSISVAAEPMDSKMIQDLSR
PRKLSKILRTEYGWDSLASRNVWSFYNGNVLINDTLPDEISPELLSKYKE
QIIQGFYWAVKEGPLAEEPIYGVQYKLLSISVPSDVNIDVMKSQIIPLMK
KACYVGLLTAIPILLEPIYEVDITVHAPLLPIVEELMKKRRGSRIYKTIK
VAGTPLLEVRGQVPVIESAGFETDLRLSTNGLGMCQLYFWHKIWRKVPGD
VLDKDAFIPKLKPAPINSLSRDFVMKTRRRKGISTGGFMSNDGPTLEKYI
SAELY
Ligand information
>5gan Chain U (length=141) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cagcuuuacagaucaauggcggagggaggucaacaucaagaacugugggc
gccuauagaacuuauaacgaacaugguucuugccuuuuaccagaaccauc
cggguguugucuccauagaaacagguaaagcuguauuuuuu
<<<<<<<<<.......<.......<<<<<.<<<<<<<<<<..<<<<<<<.
.>>>>>>>..>>>.........<<<<<<<<...........>>>>>>>>.
..>>>>>>>>>>>>......>...>>>>>>>>>........
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB5gan Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 angstrom resolution.
Resolution3.7 Å
Binding residue
(original residue number in PDB)		K111 R160 D163 K173 K182 I185
Binding residue
(residue number reindexed from 1)		K10 R59 D62 K72 K81 I84
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0030623 U5 snRNA binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000349 generation of catalytic spliceosome for first transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071007 U2-type catalytic step 2 spliceosome

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:5gan, PDBe:5gan, PDBj:5gan
PDBsum5gan
PubMed26829225
UniProtP36048|SN114_YEAST Pre-mRNA-splicing factor SNU114 (Gene Name=SNU114)

[Back to BioLiP]