Structure of PDB 4xlr Chain C Binding Site BS01

Receptor Information
>4xlr Chain C (length=1117) Species: 271 (Thermus aquaticus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EIKRFGRIREVIPLPPLTEIQVESYKKALQADVPPEKRENVGIQAAFKET
FPIEEGDKGKGGLVLDFLEYRIGDPPFSQDECREKDLTYQAPLYARLQLI
HKDTGLIKEDEVFLGHLPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPD
PARPGRYIASIIPLPKRGPWIDLEVEASGVVTMKVNKRKFPLVLLLRVLG
YDQETLVRELSAYGDLVQGLLDEAVLAMRPEEAMVRLFTLLRPGDPPKKD
KALAYLFGLLADPKRYDLGEAGRYKAEEKLGVGLSGRTLVRFEDGEFKDE
VFLPTLRYLFALTAGVPGHEVDDIDHLGNRRIRTVGELMADQFRVGLARL
ARGVRERMVMGSPDTLTPAKLVNSRPLEAALREFFSRSQLSQFKDETNPL
SSLRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGL
ITSLAAYARVDALGFIRTPYRRVKNGVVTEEVVYMTASEEDRYTIAQANT
PLEGDRIATDRVVARRRGEPVIVAPEEVEFMDVSPKQVFSLNTNLIPFLE
HDDANRALMGSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDG
EVVKVDGTRIAVRYEDGRLVEHPLRRYARSNQGTAFDQRPRVRVGQRVKK
GDLLADGPASEEGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYT
SIHIERYEIEARDTKLGPERITRDIPHLSEAALRDLDEEGIVRIGAEVKP
GDILVGRTSFKGEQEPSPEERLLRSIFGEKARDVKDTSLRVPPGEGGIVV
GRLRLRRGDPGVELKPGVREVVRVFVAQKRKLQVGDKLANRHGNKGVVAK
ILPVEDMPHLPDGTPVDVILNPLGVPSRMNLGQILETHLGLAGYFLGQRY
ISPVFDGATEPEIKELLAEAFNLYFGKRQGEGFGVDKREKEVLARAEKLG
LVSPGKSPEEQLKELFDLGKVVLYDGRTGEPFEGPIVVGQMFIMKLYHMV
EDKMHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQE
MLTIKSDDIEGRNAAYQAIIKGEDVPEPSVPESFRVLVKELQALALDVQT
LDEKDNPVDIFEGLASK
Ligand information
>4xlr Chain O (length=48) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cttgacaaaagtgttaaattgtgctatactgggagctgtcacggatgc
Receptor-Ligand Complex Structure
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PDB4xlr CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.
Resolution4.3 Å
Binding residue
(original residue number in PDB)		R142 I163 P166 K167 G169 P170 W171 N187 R243 D246 P247 Y256 L260 R266 D326 R331 L418 E421 R422 V427
Binding residue
(residue number reindexed from 1)		R141 I162 P165 K166 G168 P169 W170 N186 R242 D245 P246 Y255 L259 R265 D325 R330 L417 E420 R421 V426
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4xlr, PDBe:4xlr, PDBj:4xlr
PDBsum4xlr
PubMed26349034
UniProtQ9KWU7|RPOB_THEAQ DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)

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