Structure of PDB 4wce Chain C Binding Site BS01

Receptor Information

>4wce Chain C (length=199) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

NYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASLRQGTHAVK
NRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKV
LVVTENEDVNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKK

Ligand information

>4wce Chain X (length=2708)

gauuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccaau
gaaggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuu
gauccagagauuuccgaauggggaaacccagcaugaguuaugucauguua
ucgauauguauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuaugaguuacaaaggacgacauuagacgaaucaucuggaaag
augaaucaaagaagguaauaauccuguagucgaaaauguugucucucuug
aguggauccugaguacgacggagcacgugaaauuccgucggaaucuggga
ggaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccagu
accgugagggaaaggugaaaagcaccccggaaggggagugaaauagaacc
ugaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcgu
gccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagca
guaaauguggagccguagcgaaagcgagucugaauagggcguuuaguauu
uggucguagacccgaaaccaggugaucuacccuuggucagguugaaguuc
aggcugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaa
cugaggguagcggagaaauuccaaucgaaccuggagauagcugguucucu
ccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagagc
acuguuuggacgaggguuaccgaauucagacaaacuccgaaugccaauua
auuuaacuugggagucagaacaugggugauaagguccguguucgaaaggg
aaacagcccagaccaccagcuaaggucccaaaauauauguuaaguggaaa
aggauguggcguugcccagacaacuagcucacuagucgagugacacugcg
ccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccuu
ggacaaugguaggagagcguucuaagggcguugaagcaugaucguaagga
cauguggagcgcuuagaagugagaaugccggugugaguagcgaaagacgg
gugagaaucccguccaccgauugacuaagguuuccagaggaaggcucguc
cgcucuggguuagucggguccuaagcugaggccgacaggcguaggcgaug
gauaacagguugauauuccuguaccaccuauaaucguaucgaugggggga
cgcaguaggauaggcgaagcgugcgauuggauugcacgucuaagcaguag
gcgaguaauccgguacucgggcugagcugugauggggagaagagagucgu
ugauuucacacugccgagaaaagccucuagauagaauaggugcccguacc
gcaaaccgacacagguagucaagaugagaauucuaaggugagcgagcgaa
cucucguuaaggaacucggcaaaaugaccccguaacuucgggagaagggg
ugcucuuuaggguuaacgcccagaagagccgcagugaauaggcccaagcg
acuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguauag
gggcugacgccugcccgugcuggaagguuaagaggagugguuagcugcga
agcuacgaaucgaagccccaguaaacggcggcuaagguagcgaaauuccu
ugucggguaaguuccgacccgcacgaaaggcguaacgauuugggcacugu
cucaacgagagacucggugaaaucauaguaccugugaagaugcagguuac
ccgcgacaggacggaaagaccccguggagcuuuacuguagccugauauug
aaauucggcuuucuaacccgcaccacuuaucguggugggagacaguguca
agcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgc
ucaaagguucccucagaaugguugaaaucauucauagaguguaaaggcau
aagggagcuugacugcgagaccuacaagucgagcagggucgaaagacgga
cuuagugauccggugguuccgcauggaagggccaucgcucaacggauaaa
agcuaccccggggauaacaggcuuaucucccccaagaguucacaucgacg
gggagguuuggcaccucgaugucggcucaucgcauccuggggcuguaguc
ggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcugggu
ucagaacgucgugagacaguucggucccuauccgucgugggcguaggaaa
uuugagaggagcuguccuuaguacgagaggaccgggauggacauaccucu
gguguaccaguugucgugccaacggcauagcuggguagcuauguguggac
gggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagau
uucccaacuugguuauaagaucccucaaagaugaugagguuaauagguuc
gagguggaagcauggugacauguggagcugacgaauacuaaucgaucgaa
gacuuaau

..<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.
<<<<<<<<<.......>>>>>>>.>>.>>....<<<<<......<.....
.>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<...<..........<..<<<<<<.<<<...<<<<<......>
>>>>((......<<<.....))>>>...>>>...>>>>>>...>...>.>
>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..)))......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<....>>>>..............
....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>..
.<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<.
......>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<
<<.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>
>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<
<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<.
..<<<<<<<<...<......>...>>>>>>>>..>>.>.....>>>>.>>
>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>....<<<
......>>>...<<<<.<<<...<<<(((.....<<<<<<..........
.<<..<<<<.<<<<........<<<<......>>>>....>>>>.>>>>.
>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<..
>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<....>>.
>>>>>...>>.>>>>>>>..>.....>>>>>><......<<....<<<<<
<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>
>...<<<<<........>>>>>))<<<<<<<......<<<...<<<<...
.<<<<...<<...<......<<<...{.{...>>>...<....<<<<<.<
<<<<<<........>>>>..>>>..>>>>>.....>..............
>....>>...>>>>.........>>>.>.>>>...>>>>>>>>>>>..>>
.....<<......>>>>>>>.<<<<....>.>>>..>>>>..<<<<....
<<<<<<<<.<<...............<<<<<...............>>>>
><<<<<<<.<<<<....>>>>.>>>>>>>............<<<<<<<<<
..<<<.........>>>..<<<<<<<.....<<<....>>>.......>>
>>>>>.<<<<<..<<.<<<<<<<..<<<<..<<...<<<<<..<<.>>..
.>>>>>...>>..>>>>>>>>>..>>>>...........<..........
.<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>.....
>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<
<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<.<<<<<<.<
<<<.<..>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>
.>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<......>>>>>>.....<<.[.(((>>..
..>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>
)))]......<<<<<<<.((..>>>>..))>>>>>>>.............
>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>>...>
>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......
>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>.
...<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<
<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<
<<<...<<<<<<<<<<<.}.}>>>>>>..>>>>>...>>>>.>>>>>...
<<<....<<<<<...........>>>>>....>>>.>>>>.......>>>
>>>>.<<<<.>>>>....<<<<<<<<<.......>>>>>....<<<<<<<
<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>...
.>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wce Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
• Resolution: 3.526 Å
• Binding residue (original residue number in PDB): V32 R41 S43 R45 Q46 T48 H49 R54 S55 V57 S58 G60 K66 Q67 K68 G69 G71 R72 A73 R74 Q75 G76 R79 Q82 W83 R84 G85 I88 V89 F90 P92 R95 Y99 K100 M101 P102 K103 K104 M105 R106 P134 V161 E164
• Binding residue (residue number reindexed from 1): V30 R39 S41 R43 Q44 T46 H47 R52 S53 V55 S56 G58 K64 Q65 K66 G67 G69 R70 A71 R72 Q73 G74 R77 Q80 W81 R82 G83 I86 V87 F88 P90 R93 Y97 K98 M99 P100 K101 K102 M103 R104 P132 V159 E162

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wce, PDBe:4wce, PDBj:4wce
• PDBsum: 4wce
• PubMed: 26464510
• UniProt: Q2FW07|RL4_STAA8 Large ribosomal subunit protein uL4 (Gene Name=rplD)