Structure of PDB 4m27 Chain C Binding Site BS01

Receptor Information

>4m27 Chain C (length=336) Species: 58340 (Streptomyces lavendulae subsp. lavendulae)

TPSYSLTPAEASAVAELTLELAAAYGSFGDPVLLRDLPRLAARLPEGVQD
FLREFKLADRHGHTVIRGHDFDQRRIGPTPDHWRGRVRPGPEFPEELLLM
LYSALLGEPFGWATQQDGHLVHDIFPIRSHENDQLGMGSKQLLTWHTEDA
FHPYRSDYLILGALRNPDHVPTTVGELDLSSLSAEDIDVLFEPRYHIAPD
ESHLPKNNTIATEEEAARFATIQRMIDERPLGPLLYGSRLDPYMRLDPYF
TSVPQDDTDARRAYDALFKVVDSGMREVVADQGDVLFIDNHRAVHGRLPF
QARYDGTDRWLKRVCVTSDLRRSREMRATSATRLLG

Ligand information

• Ligand ID: ARG
• InChI: InChI=1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/p+1/t4-/m0/s1
• InChIKey: ODKSFYDXXFIFQN-BYPYZUCNSA-O
• SMILES:
  OpenEye OEToolkits 1.5.0: C(CC(C(=O)O)N)CNC(=[NH2+])N
  CACTVS 3.341: N[C@@H](CCCNC(N)=[NH2+])C(O)=O
  OpenEye OEToolkits 1.5.0: C(C[C@@H](C(=O)O)N)CNC(=[NH2+])N
  CACTVS 3.341: N[CH](CCCNC(N)=[NH2+])C(O)=O
  ACDLabs 10.04: O=C(O)C(N)CCCN\C(=[NH2+])N
• Formula: C6 H15 N4 O2
• Name: ARGININE
• PDB chain: 4m27 Chain C Residue 401

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4m27 Biosynthesis of streptolidine involved two unexpected intermediates produced by a dihydroxylase and a cyclase through unusual mechanisms.
• Resolution: 2.35 Å
• Binding residue (original residue number in PDB): Q123 L143 G144 H154 E156 D208 D255 R321
• Binding residue (residue number reindexed from 1): Q115 L135 G136 H146 E148 D200 D247 R313
• Annotation score: 5

Enzymatic activity

• Enzyme Commision number: 1.14.11.41: L-arginine hydroxylase.

Gene Ontology

Molecular Function

• GO:0005506 iron ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0102525 2-oxoglutarate, L-arginine oxygenase (succinate-forming) activity

External links

• PDB: RCSB:4m27, PDBe:4m27, PDBj:4m27
• PDBsum: 4m27
• PubMed: 24505011
• UniProt: G9MBV2