Structure of PDB 2y7h Chain C Binding Site BS01

Receptor Information

>2y7h Chain C (length=529) Species: 562 (Escherichia coli)

MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETGQEAEYL
PEGYRWDDLKSRIGQEQLQFYRKMLVHLGEDDKKLVQAVFHNVSTTITEP
KQITALVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYF
TPRPLIKTIIHLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLD
GDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDHGGAIRLGNTL
GSDGENLPKAHIVATNPPFGSAAGTNITRTFVHPTSNKQLCFMQHIIETL
HPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHLHTILRLPTGIFYAQGV
KTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTPFTDEHLQP
FERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
SREWIRTAKSDSLDISWLKDKDSIDADSLPEPDVLAAEAMGELVQALSEL
DALMRELGASDEADLQRQLLEEAFGGVKE

Ligand information

>2y7h Chain E (length=20)

gttgcacgtcgacgttgaac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2y7h The Structure of M.Ecoki Type I DNA Methyltransferase with a DNA Mimic Antirestriction Protein.
• Resolution: 18.0 Å
• Binding residue (original residue number in PDB): Q148 N266 P267 F269 G270 N313 F316 F345 V350
• Binding residue (residue number reindexed from 1): Q148 N266 P267 F269 G270 N313 F316 F345 V350

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0005515 protein binding
• GO:0008168 methyltransferase activity
• GO:0008170 N-methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

Cellular Component

• GO:0005829 cytosol
• GO:0019812 type I site-specific deoxyribonuclease complex

External links

• PDB: RCSB:2y7h, PDBe:2y7h, PDBj:2y7h
• PDBsum: 2y7h
• PubMed: 19074193
• UniProt: P08957|T1MK_ECOLI Type I restriction enzyme EcoKI methylase subunit (Gene Name=hsdM)