Structure of PDB 4v6i Chain Bs Binding Site BS01

Receptor Information

>4v6i Chain Bs (length=257) Species: 4932 (Saccharomyces cerevisiae)

MGGIREKKAEYFAKLREYLEEYKSLFVVGVDNVSSQQMHEVRKELRGRAV
VLMGKNTMVRRAIRGFLSDLPDFEKLLPFVKGNVGFVFTNEPLTEIKNVI
VSNRVAAPARAGAVAPEDIWVRAVNTGMEPGKTSFFQALGVPTKIARGTI
EIVSDVKVVDAGNKVGQSEASLLNLLNISPFTFGLTVVQVYDNGQVFPSS
ILDITDEELVSHFVSAVSTIASISLAIGYPTLPSVGHTLINNYKDLLAVA
IAASYHY

Ligand information

>4v6i Chain DA (length=3354)

guuugaccucaaaucagguaggaguaccgcugaacuuaagcauaucaaua
agcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaag
cggcaaaagcucagaaaucugguaccuucggugcccgaguuguaauuugg
agagggcacuuuggggccguuccuugucuauguuccuuggaacaggacgu
cauagagggugagaaucccguguggcgaggagugcgguucuuuguaaagu
gccuucgaagagucgaguuguuugggaaugcagcucuaagugggugguaa
auuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaaguaca
gugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacguga
aauuguugaaagggaagggcauuugaucagacaugguguuuugugcccuc
ugcuccuuguggguaggggaaucucgcauuucacugggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaaagugaaagguuggggccucgcaagaggugcacaaucgaccgauccug
augucuucggauggauuugaguaagagcauagcuguugggacccgaaaga
uggugaacuaugccugaauagggugaagccagaggaaacucugguggagg
cucguagcgguucugacgugcaaaucgaucgucgaauuuggguauagggg
cgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuucccu
caggauagcagaagcucguaucaguuuuaugagguaaagcgaaugauuag
agguuccggggucgaaaugaccuugaccuauucucaaacuuuaaauaugu
aagaaguccuuguuacuuaauugaacguggacauuugaaugaagagcuuu
uagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaac
guagaguuaaggugccggaauacacgcucaucagacaccacaaaaggugu
uaguucaucuagacagccggacgguggccauggaagucggaauccgcuaa
ggaguguguaacaacucaccggccgaaugaacuagcccugaaaauggaug
gcgcucaagcguguuaccuauacucuaccgucaggguugauaugaugccc
ugacgaguaggcaggcguggaggucgugacgaagccagacguaaggucgg
gucgaacggccucuagugcagaucuuggugguaguagcaaauauucaaau
gagaacuuugaagacugaaguggggaaagguuccacgucaacagcaguug
gacguggguuagucgauccuaagagauggggaagcuccguuucaaaggcc
ugauuuuaugcaggccaccaucgaaagggaauccgguuaagauuccggaa
ccugguauggauucuucacggaacguaacugaauguggagacgucggcgc
gagcccugggaggaguuaucuuuucuucuuaacagcuuaucaccccggaa
uugguuuauccggagaugggucuuauggcuggaagaggccagcaccuuug
cuggcuccggugcgcuugugacggcccgugaaaauccacaggaaggaaua
guuuuaugccaggucguacugauaaccgcagcaggucuccaaggugaaca
gccucuaguugauagaauaauguagauaagggaagucggcaaaauagauc
cguaacuucgggauaaggauuggcucuaagggucggguagugagggccuu
ggucagacgcagcgggcgugcuuguggacugcuugguggggcuugcucug
cuaggcggacuacuugcgugccuuguuguagacggccuugguaggucucu
uguagaccgucgcuugcuacaauuaacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
gugauguugacgcaaugugauuucugcccagugcucugaaugucaaagug
aagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacucucu
uaagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggau
uaacgagauucccacugucccuaucuacuaucuagcgaaaccacagccaa
gggaacgggcuuggcagaaucagcggggaaagaagacccuguugagcuug
acucuaguuugacauugugaagagacauagaggguguagaauaaguggga
gcuucggcgccagugaaauaccacuaccuuuauaguuucuuuacuuauuc
aaugaagcggagcuggaauucauuuuccaaaggucccauucggggcugau
ccggguugaagacauugucagguggggaguuuggcuggggcggcacaucu
guuaaacgauaacgcagauguccuaaggggggcucauggagaacagaaau
cuccaguagaacaaaaggguaaaagcccccuugauuuugauuuucagugu
gaauacaaaccaugaaaguguggccuaucgauccuuuaguccucggaauu
ugaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggc
agucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucu
uccuaucauaccgaagcagaauucgguaagguuggauuguucacccacua
auagggaacgugagcuggguuuagaccgucgugagacagguuaguuuuac
ccuacugaugauguuaccgcaauaguaauugaacuuaguacgagaggaac
aguucauucggauaauugguuuuugcggcgucugaucaggcauugccgcg
aagcuaccauccgcuggauuauggcugaacgccucuaagucagaauccau
gcuagaacgcggugauuucuuugcuccacacaauauagauggauacgaau
aaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugcac
uuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuuugc
gugggauaaucauuuguauacgacuuagauguacaacggggauuguaagc
aguagaguagccuuguuguuacgaucugcugagauuauuuguugucugau
uugu

..........................<<<<<.....<......>......
>>>.>.>.........<<....>>..<<<<<......<<.....>>....
.>>>>>..<<<......<<.<<<<<<...>>>>>>.>>............
.<<<<.<<<<<<<<<<<<<<<<<<<<<<.....<<<<......>>>>.(.
......<<<......)>>>....>>>>>>>.>.>>>>>>>>....>>>>>
>>>.>>........<<<<<<<........>>>>>>>.....<<<<<..[[
.[[.>>>>>..>>>................................<.<<
....>>...>...........<<<......>>>.................
...................<<<.<<<<<<<....<<<<...<<<..<<<<
<<<.<<....>>>>>>>>>......>>>...>>>>..<<<<<<<.<<<<<
..<.<<<<<<.<<...<<<.<<<<..<<<<<<<<<<....>>>>>>..>>
>>.>>>>.>>>.>>.>>>>>>..>..>>>>>....<<..<<<....>>>.
>>..>>>>>>>.><.>>>>>>.>>>....<<<<........>>>><<<<<
<...<.<<<<<.<<<<<<..<<...<<<<<.....>>>>>...>>...<<
....>>.....<<<<..<<<<<....>>>>>...>>>>....<<<<<<..
..<....>....>>>>.>>.......>>>>>>.>>>>>.>.<<.<...<<
<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....
>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>>>...<<
.......>>......>>>>>>......>.>>..<<<<<<....<.<<<..
..>>>.><.<<<<<<<..........<<.<<......<<..........<
..<......<.............>....>..>........>>.......>
>.>>..<..<<<<..<......>...>>>>..>...........>>>>>>
>..>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...
<<<<<<<..<<<<(((.....<<<<<<..................<<..<
<<<<<<<<.......<<<<<.<<....<<.........>>....>>....
.....<<......>>...>>>>>...>>>>>>>>>.>>...........)
))......>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>
>>>>>....>>>>>><..<<<<<<<<<.....<.<<.<.<....>.>.>>
.>...>>>>>>>>>...>.....>>>>>><<.....<<.....<<<<<<.
......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>>
.>>>>>>....((<<<<<<...<<....<<<....>>>........<<<<
<<........>>>>>>....>>....>>>..<<<<<........>>>>>)
)<<<<<<<.<<.................<<...<<<<<<...<<<<.<<<
<.<..<<<<<<<<<<.......>>>>>><...<.<......<...<....
.<<<.....>>>.......>..>......>.>....>.<<<<<<....>>
>>>>..>>>>.....>>>>>.>>>>.........>>>>>>...>......
>.>>.>>>>>>>.>>>..>>.....<<......>>>>>>>..<<<.....
>>>...>>>>..<<<<.....<<<<<<<.<<...............<<<<
<.....<..>......>>>>><<.<<<<<<<<<<<.<<<<<<<<.<<<..
<<<<....<<<<<<<<<<<<<..<<<<.<<<<<<<<<<<<<....>>>>>
>>>>>>>>.>>>>..>>>>>>>.>>>>>>....>>>>..>>>.<<<<<..
...>>>>>....>>>>>>>>......>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<...
.>>>.......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<
<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>.
.......<...........<<<<<.......>>>>>.....>.....>>.
...>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<
<........>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..
<<<<<.<<<<<<<<..<<<<<<<<<<<<<<<<<..........<<<<<..
<<....>>............>>>>>.>>>>>>>.>>>>>>>>>>...<<<
<<.....<<<<..<<<<<......>>>>>..<<<<<<....>>>>>>..>
>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>>...<<<..<<<<.......
>>>>..>>>.<<<....>>>.>>))).....<.<<<<<<.<<<<.]].]]
.>>>>>>>>>>>.........>>>>..>>>>>>.......<<.<...<<<
<<<...<<.......>>...>>>>>>....>..>>.......<<<<<..<
<<<<..<.<<<<<<<......>>>>>>>..>.<<<.........>>>...
..>>>>>......>>>.>>....<<<<<<<....>>>.>>>>....>>..
>>>.>>>>>....<<<<<<...<<<<.<.<<<<<<...............
>>>>>>.><<<<<....<<<...<<<<<.<<<<....>>>>....>>>>>
..>>>...>>>>>.<<<<...<<<<<...........>>>>>...>>>>.
>>>>.....>>>>>>.........<<<<<.<<<<......<.........
...<<.<......>>>..........<.......>.......<<<<<<.<
<.<<<......>>>..>>>>>>>.>......(....>..>>)....>>>.
>>>>>..<<<<.<.............>.....<<<<<<<...<<....<<
<<....<<<<<......>>>>>...>>>>...>>...>>.>>>>>..>>>
>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v6i Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
• Resolution: 8.8 Å
• Binding residue (original residue number in PDB): M1 K8 A9 F12 V30 D31 V33 S34 S35 Q36 H39 R42 R46 V51 L52 M53 G54 K55 N56 N83 V84 G85 R110
• Binding residue (residue number reindexed from 1): M1 K8 A9 F12 V30 D31 V33 S34 S35 Q36 H39 R42 R46 V51 L52 M53 G54 K55 N56 N83 V84 G85 R110

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v6i, PDBe:4v6i, PDBj:4v6i
• PDBsum: 4v6i
• PubMed: 20980660
• UniProt: P05317|RLA0_YEAST Large ribosomal subunit protein uL10 (Gene Name=RPP0)