Structure of PDB 6uz7 Chain Br Binding Site BS01

Receptor Information

>6uz7 Chain Br (length=195) Species: 28985 (Kluyveromyces lactis)

EKKAEYFAKLREYLEEYKSVFVVGVDNVSSQQMHEVRKNLRGRAVVLMGK
NTMVRRAVRGFISDFPDYEKLLPFVKGNVGFIFTNDSLQDIKEVIIANKV
AAPARAGAVAPEDIWVLAVNTGMEPGKTSFFQALGVPTKIARGTIEIVSD
VKVVDAGKRVGASEASLLNLLNISPFTYGLTVVQVYDNGQVFPAS

Ligand information

>6uz7 Chain 5 (length=3269)

uugaccucaaaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucuggcgucuucgacguccgaguuguaau
uugaagaaggcaacuuuguagcugguccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggaucccaguuauuugu
aaagugcuuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugggccagcauc
aguuuuagcgguuggauaaauccucgggaauguggcucugcuucgguaga
guguuauagcccgugggaauacagccagcugggacugaggauugcgacuu
uugucaaggaugcuggcguaaugguuaaaugccgcccgucuugaaacacg
gaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccguacg
cguaaugaaagugaacguaggugagggcccgcaagggugcaucaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
ugauuagagguaccgggguugaaaugaccuugaccuauucucaaacuuua
aauauguaagaaguccuuguugcuuaauugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguggaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacuccaccgucaggguuaauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuaggcu
guaaagcuggguagaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uucaaaggccuaauuuucuaggccaccaucgaaagggaauccgguuaaua
uuccggaaccuggauauggauucuucacgguaacguaacugaauguggag
acgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuau
caccccggaauugguuuauccggagagggggucuuauggcuggaagagcc
cagcccuugugcuggguccggugcgcccgcgacggcccuugaaaauccac
aggaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucu
ccaaggugaacagccucuaguugauagaauaauguagauaagggaagucg
gcaaaauagauccguaacuucgggauaaggauuggcucuaaggaucggaa
gugagggccuuggucagacgcggcguuguagacggccuugguaggucucu
uguagaucugcaauuaacgaucaacuuagaacugguacggacaaggggaa
ucugacugucuaauuaaaacauagcauugcgauggucagaaagugauguu
gacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaauu
caaccaagcgcggguaaacggcgggaguaacuaugacucucuuaagguag
ccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgaga
uucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacgg
gcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuagu
uugacauugugaagagacauagaggguguagcauaagugggagcuucggc
gccagugaaauaccacuaccuuuauaguuucuuuacuuauucaauuaagc
ggagcuggaauucauuuuccacguucuagcauucaaaguccuauacgggc
ugauccggguugaagacauugucagguggggaguuuggcuggggcggcac
aucuguuaaacgauaacgcagauguccuaagggggacucauggagaacag
aaaucuccaguagaacaaaaggguaaaaguccccuugauuuugauuuuca
gugugaauacaaaccaugaaaguguggccuaucgauccuuuagguccucg
gaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggcuu
guggcagucaagcguucauagcgacauugcuuuuugauucuucgaugucg
gcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguucac
ccacuaauagggaacgugagcuggguuuagaccgucgugagacagguuag
uuuuacccuacugaugaauguuaucgcaauaguaauugaacuuaguacga
gaggaacaguucauucggauaauugguuuuugcggcugucugaucaggca
uugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaaguca
gaauccaugcuagaacgcgaugauuucuuugccuugcacaauauagaagg
auaagaauaaggcgucuuuuuggcgucgcugaaccauagcaggcuggcaa
cggugcucuuagcggaaaggcuuugggugcuugccggcgaauugcaaugu
cauuuugcgcaaggauaaaucauuuguauacgacuuaaauguacaacagg
guauuguaagcaguagaguagccuuguuguuacgaucugcugagauuaag
ccuucguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<........<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<.<<<<......<<<<<<<....>>>>>
>>.......>>>>.>>.>.>.>>>>>.>>>>>>>>>>........<<<..
..>>>.....>>>>>>>.....>>>>>>>>>>>..<<<<........>>>
><<<<<<.....<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>
>...<<....>>.......<<<<<..<<<<....>>>>...>>>>>.<<<
<<<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>...<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<<<<<<<<<....<<.<<<<<<<.....<<<....
<<<....<<<<..<<<<<<<.....>>>>>>>>>>>....>>>..>.>>.
....>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>..
>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<.....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
......<<<<<<......>>>>>>....>>....>>...<<<<<......
..>>>>>))<<<<.<<<<<<<...<.<<<....>>>.......<<<<<<.
..<<<<.<<<<.<<.<<<<<<<<<<..{{{..>>>>>>....<<<<<...
.<<<<<.....<<<.....>>>.....>>>>>.....>>>>>.....<<<
<<<<......>>>>>>>>>>>...>>.>>>>.>>>>.........>>>>>
>.....>....>>>..>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.......
..<.....<<<<<...............>>>>><<.<<<<<<<<<<<...
..<...<<<..<<<<....<<<<..>>>>....>>>>..>>>........
.......>.........>>>>>..>>>>>>.>>............<<<<<
<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>...
...>>>>>>>...<<..<<<..<<<<<<....<<<....<<<<<....>>
>>.>...>>>.>>>>..>>>>><<<<<<.......>>>>>>........<
...........<<<<<.......>>>>>.....>.....>>....>>.>>
>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<.......
.>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<
<<<<<<..<<<<<..<.....<<<<..........<<<<<..<<....>>
............>>>>>.>>>>.....>...>>>>>...<<<<<.....<
<<<................................<..<.<.....>>..
>..>>>>..>>>>>.....>>>>>>>>..>>>>>....<<.....>>...
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<...
....>>>>.>>>>.<<<....>>>.>>))).......((((((..((((.
>>>>>>))))))))))..........]]]]..}}]]]].......<<.<.
<.<<<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<
<<..<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........
>>>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>..
..}}..}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<........
.......>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>
..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>
...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<<
..........<<<<<<.....>>>>>>......>>.......<<<<<<..
.<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>............
.>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<<<
<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>....
>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uz7 Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): D33 N34 S36 S37 Q38 H41 R44 K45 V53 L54 M55 K57 V61 G84 N85
• Binding residue (residue number reindexed from 1): D26 N27 S29 S30 Q31 H34 R37 K38 V46 L47 M48 K50 V54 G77 N78

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6uz7, PDBe:6uz7, PDBj:6uz7
• PDBsum: 6uz7
• PubMed: 31900355
• UniProt: Q6CW89