Structure of PDB 7zrs Chain Bj Binding Site BS01

Receptor Information

>7zrs Chain Bj (length=77) Species: 4932 (Saccharomyces cerevisiae)

AREITDIKQFLELTRRADVKTATVKINKKLNKAGKPFRQTKFKVRGSSSL
YTLVINDAGKAKKLIQSLPPTLKVNRL

Ligand information

>7zrs Chain 5 (length=3185)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacugggccagcaucaguuuugguggcaggauaaauccauaggaaug
uagcuugccucgguaaguauuauagccugugggaauacugccagcuggga
cugaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccg
cccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugg
guguaaaacccauacgcguaaugaaagugaacguagguuggggccucgca
agaggugcacaaucgaccgauccugaugucuucggauggauuugaguaag
agcauagcuguugggacccgaaagauggugaacuaugccugaauagggug
aagccagaggaaacucugguggaggcucguagcgguucugacgugcaaau
cgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagu
agcugguuccugccgaaguuucccucaggauagcagaagcucguaucagu
uuuaugagguaaagcgaaugauuagagguuccggggucgaaaugaccuug
accuauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaa
cguggacauuugaaugaagagcuuuuagugggccauuuuugguaagcaga
acuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacac
gcucaucagacaccacaaaagguguuaguucaucuagacagccggacggu
ggccauggaagucggaauccgcuaaggaguguguaacaacucaccggccg
aaugaacuagcccugaaaauggauggcgcucaagcguguuaccuauacuc
uaccgucaggguugauaugaugcccugacgaguaggcaggcguggagguc
agugacgaagccuagaccguaaggucgggucgaacggccucuagugcaga
ucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagug
gggaaagguuccacgucaacagcaguuggacguggguuagucgauccuaa
gagauggggaagcuccguuucaaaggccugauuuuaugcaggccaccauc
gaaagggaauccgguuaagauuccggaaccuggauauggauucuucacgg
uaacguaacugaauguggagacgucggcgcgagcccugggaggaguuauc
uuuucuucuuaacagcuuaucaccccggaauugguuuauccggagauggg
gucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugu
gacggcccgugaaaauccacaggaaggaauaguuuucaugccaggucgua
cugauaaccgcagcaggucuccaaggugaacagccucuaguugauagaau
aauguagauaagggaagucggcaaaauagauccguaacuucgggauaagg
auuggcucuaagggucggguacgaucaacuuagaacugguacggacaagg
ggaaucugacugucuaauuaaaacauagcauugcgauggucagaaaguga
uguugacgcaaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaac
gagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggga
acgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacuc
uaguuugacauugugaagagacauagagggcuaccuuuauaguuucuuua
cuuauucaaugaagcggagcuggaauucauuuuccacguucuagcauuca
aggucccauucggggcugauccggguugaagacauugucagguggggagu
uuggcuggggcggcacaucuguuaaacgauaacgcagauguccuaagggg
ggcucauggagaacagaaaucuccaguagaacaaaaggguaaaagccccc
uugauuuugauuuucagugugaauacaaaccaugaaaguguggccuaucg
auccuuuagucccucggaauuugaggcuagaggugccagaaaaguuacca
cagggauaacuggcuuguggcagucaagcguucauagcgacauugcuuuu
ugauucuucgaugucggcucuuccuaucauaccgaagcagaauucgguaa
gcguuggauuguucacccacuaauagggaacgugagcuggguuuagaccg
ucgugagacagguuaguuuuacccuacugaugaauguuaccgcaauagua
auugaacuuaguacgagaggaacaguucauucggauaauugguuuuugcg
gcugucugaucaggcauugccgcgaagcuaccauccgcuggauuauggcu
gaacgccucuaagucagaauccaugcuagaacgcggugauuucuuugcuc
cacacaauauagauggauacgaauaaggcguccuuguggcgucgcugaac
cauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcuugc
uggcgaauugcaaugucauuuugcguggggauaaaucauuuguauacgac
uuagauguacaacgggguauuguaagcaguagaguagccuuguuguuacg
aucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.................
.........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<....
..<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>
>>>........<<<....>>>.....>>>>>>>.....>>>>>.>>>>>.
.<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<
<<.....>>>>...>>>...<<....>>.......<<<<<..<<<<<...
.>>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>...
.>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>
>>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>...>
..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<
<<<<<<.....<<<....<<<..<.<<<<...<<<<<.......>>>>>.
>>>>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>
>>.>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>
>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<
<<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<<..
..<<.........>>....>>.........<<......>>...>>>>>..
.>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>
>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<
.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>....
.>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<
<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...
<<....<<<....>>>........<<<.<<........>.>>>>....>>
....>>...<<<<<........>>>>>))<<<<.<<<<<.<.....<<<.
...>>>.......<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..{{{
..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>
>>.....>>>>>....><<<<<<<......>>>>>>>>>>>.>.>>>>>>
>.>>>>.........>>>>>>..........>.>..>>>>>>>>>>>>..
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
...<<<<<<<.<<.........<.....<<<<<....<<..>>.....>>
>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<.....<<.....<<<<..
..>>>>.....>>..>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<..<<<<<<<<<<<<<<<<<....>>>>>>>.>>>>>>>>>
>...<<<<<.....<<<<..<<<<<......>>>>>..............
.<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>
...<<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<
<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>
>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))....
...((((((..((((.>>>>>>))))))))))..........]]]]..}}
]]]].......<<.<.<.<<<<<<...<<.......>>...>>>>>>...
>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>.
..>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<
<<....>>>.>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<.
<.<<<<<<...............>>>>>>.><<<<<...<<<<....<<<
<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<
...........>>>>>...>>>>.>>>>....>>>>>>>>.......<<<
<<<.<<<.<<....<<..........<<<<<<.....>>>>>>......>
>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>
>>>>.............>>.>>>.>>>>>>..<<<<<.<...........
..>.....<<<<<<<<<.<<<....<<<<....<<<<<......>>>>>.
..>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zrs Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 4.8 Å
• Binding residue (original residue number in PDB): A2 R3 R17 K26 N28 K42 K44 R46 S48 S49 S50 T53 L78
• Binding residue (residue number reindexed from 1): A1 R2 R16 K25 N27 K41 K43 R45 S47 S48 S49 T52 L77

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0022618 protein-RNA complex assembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zrs, PDBe:7zrs, PDBj:7zrs
• PDBsum: 7zrs
• PubMed: 36801861
• UniProt: P49167|RL38_YEAST Large ribosomal subunit protein eL38 (Gene Name=RPL38)