Structure of PDB 8evq Chain Bg Binding Site BS01

Receptor Information

>8evq Chain Bg (length=312) Species: 4932 (Saccharomyces cerevisiae)

EVLVLRGTLEGHNGWVTSLATSAGQPNLLLSASRDKTLISWKLTGDDQKF
GVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQRFV
GHKSDVMSVDIDKKASMIISGSRDKTIKVWTIKGQCLATLLGHNDWVSQV
RVVPNEKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTA
SPDGTLIASAGKDGEIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYWLAA
ATATGIKVFSLDPQYLVDDLRPEFAGYSKAAEPHAVSLAWSADGQTLFAG
YTDNVIRVWQVM

Ligand information

>8evq Chain B5 (length=1782)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagucc
uuguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguu
caaagcaggcguauugcucgaauauauuagcauggaauaauagaauagga
cguuugguucuauuuuguugguuucuaggaccaucguaaugauuaauagg
gacggucgggggcaucaguauucaauugucagaggugaaauucuuggauu
uauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaa
ucaagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucu
uaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugacc
cacucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguau
ggucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggag
uggagccugcggcuuaauuugacucaacacggggaaacucaccaggucca
gacacaauaaggauugacagauugagagcucuuucuugauuuugugggug
guggugcauggccguucuuaguugguggagugauuugucugcuuaauugc
gauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauuugcug
guuauccacuucuuagagggacuaucgguuucaagccgauggaaguuuga
ggcaauaacaggucugugaugcccuuagacguucugggccgcacgcgcgc
uacacugacggagccagcgagucuaaccuuggccgagaggucuugguaau
cuugugaaacuccgucgugcuggggauagagcauuguaauuauugcucuu
caacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacgu
cccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuua
gugaggccucaggaucugcuuagagaagggggcaacuccaucucagagcg
gagaauuuggacaaacuuggucauuuagaggaacuaaaagucguaacaag
guuuccguaggugaaccugcggaaggaucauu

...<<<<<.{.((((>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
<.....<<<..<<..<<....<<<..................<.....<<
.<<.......>>.>>......>.........<<<<<............>>
>>><...<<<<<<......(((.{((.<.<....>.>.....<......>
.>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>>>>.<<.<<<.......
...>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<
.....>>>..>>>..>....>.>.....<<<..<................
..>...>>>......<<..<...........>..>>.........<<<<<
((......<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>
>>>>.........<<<[[.....<.<<...<<<.<<....<<<<<<.<<<
<..............>>>>.>>>>>>......<<<<..............
.................>>>>.....<<<<<.<<.......<<...<...
....>..<<<.....>>>....>>......>>.>>..>>>.........<
<...<<<<<<<<.....)).}))).>>>>>>>>..>>.....>>....<<
<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>
.>>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>...>>
>...>>.>....<<<<<<<...<...<<<<.<.....>.>>>>...>>>>
>>>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>
>>>>>>>..>>>...<<..]]>>...>>.....>>>.>>>.<<<......
<<<<....>>>>....>>>..)))).}<<<<<.<<<<<<<..<<.<<<<<
<..<<<.<<<<<<......<<........>>..........<<<<<.<..
..<<<<<........<<.<<<........>>>.>>......>>>>>...<
<.<<<..<<.<<<<<<....<<<.<<<<<....>>>...<<<......>>
>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<.<<<<<..>>>>>
.>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>
>>....>>>.>>>.....>>>>>>>.....>.>>>>>...>>.>>>>.>>
>.....<<<<<<<......<<.....<<..<<<<....>>>>..>>....
>>.......>>>>>>>......<....<<<<<<..........>>>>>>.
...>.....>>>>>>....<<<<<<<<.......>>>>>>>>......>>
...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<<<<<.<
<<..<.<<..<...<<<<<...<<<<.<<<<....>>>>.>>>>...>>>
>>...>..>>.>..>>>.>>>>>>>>...>>.>>>>...>.>>...>...
..<<<<<<<<<<..>>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8evq Regulation of translation by ribosomal RNA pseudouridylation.
• Resolution: 2.72 Å
• Binding residue (original residue number in PDB): N17 G63 H66 R90 R102 Y281 K283 A284
• Binding residue (residue number reindexed from 1): N13 G59 H62 R86 R98 Y277 K279 A280

Gene Ontology

Molecular Function

• GO:0001965 G-protein alpha-subunit binding
• GO:0005080 protein kinase C binding
• GO:0005092 GDP-dissociation inhibitor activity
• GO:0043022 ribosome binding
• GO:0045182 translation regulator activity

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006521 regulation of cellular amino acid metabolic process
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0010629 negative regulation of gene expression
• GO:0017148 negative regulation of translation
• GO:0061157 mRNA destabilization
• GO:0070651 nonfunctional rRNA decay
• GO:0072344 rescue of stalled ribosome
• GO:1902660 negative regulation of glucose mediated signaling pathway
• GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process
• GO:1990145 maintenance of translational fidelity
• GO:2001125 negative regulation of translational frameshifting

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8evq, PDBe:8evq, PDBj:8evq
• PDBsum: 8evq
• PubMed: 37595043
• UniProt: P38011|GBLP_YEAST Small ribosomal subunit protein RACK1 (Gene Name=ASC1)