Structure of PDB 6s47 Chain Bg Binding Site BS01

Receptor Information

>6s47 Chain Bg (length=57) Species: 4932 (Saccharomyces cerevisiae)

KKVKLAVLSYYKVDAEGKVTKLRRECSNPTCGAGVFLANHKDRLYCGKCH
SVYKVNA

Ligand information

>6s47 Chain BA (length=1707)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccaacggggccuuuccuucuggccaggacuuuuacuuugaaaaaauua
gaguguucaaagcaggcguauugcucgaauauauuagcauggaauaauag
aauaggacguuugguucuauuuuguugguuucuaggaccaucguaaugau
uaauagggacggucgggggcaucaguauucaauugucagaggugaaauuc
uuggauuuauugaagacuaacuacugcgaaagcauuugccaaggacguuu
ucauuaaucaagaacgaaaguuaggggaucgaagaugaucagauaccguc
guagucuuaaccauaaacuaugccgacuagggaucgggugguguuuuuuu
aaugacccacucggcaccuuacgagaaaucaaagucuuuggguucugggg
ggaguauggucgcaaggcugaaacuuaaaggaauugacggaagggcacca
ccaggaguggagccugcggcuuaauuugacucaacacggggaaacucacc
agguccagacacaauaaggauugacagauugagagcucuuucuugauuuu
gugggugguggugcauggccguucuuaguugguggagugauuugucugcu
uaauugcgauaacgaacgagaccuuaaccuacuaaauaguggugcuagca
uuugcugguuauccacuucuuagagggacuaucgguuucaagccgaugga
aguuugaggcaauaacaggucugugaugcccuuagacguucugggccgca
cgcgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucu
ugguaaucuugugaaacuccgucgugcuggggauagagcauuguaauuau
ugcucuucaacgaggaauuccuaguaagcgcaagucaucagcuugcguug
auuacgucccugcccuuuguacacaccgcccgucgcuaguaccgauugaa
uggcuuagugaggccucaggaucugggagaauuuggacaaacuuggucau
uuagaggaacuaaaagucguaacaagguuuccguaggugaaccugcggaa
ggaucau

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[..................<.....<<
.<<<.....>>>.>>......>.........<<<<<..<<....>>..>>
>>>[...((((((......<<<<<<<<<<......>>....<<......>
>)))))).....]...<<<<..<<<.....>>>.>>>>....]]]...}}
}}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<.......
...>>>.>>.<.<<....>>.>...]]]]]].........<<<....<<<
.....>>>..>>>...............<<.<<....<..........>.
...>>..>>......<<..<...........>..>>.........<<<<<
((....<.<<<<.....<<..))>>.......>>>>>>>>>>..))))))
]]]].........[[[{{.......{{...[[[.[[....<<<<<<.<<<
<..>>>>.>>>>>>....<<<<<..>>>>>...<<<<<.<<.......<<
...<.......>..<<<.....>>>....>>......>>.>>..>>>...
.......[[..((((((((....>>>>>>>>.)))))))).]]......]
]....<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>
......>.>>>>>>..>>.......<<....>>...>>>>...>>>>>.>
>>...]]]...}}......<<<<<<<...<...<<<<.<.....>.>>>>
...>>>>>>>>..........<<<.<<.<<<..<<<<<<<<.<<<<....
.>.>>>>>>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<
<......<<<<....>>>>....>>>..]]]]..<<<<<.<<<<<<<..<
<.<<<<<<..<<<.<<<<<<......<<........>>..........<<
<<<.<....<<<<<........<<.<<<........>>>.>>......>>
>>>...<<.<<<..<<.<<<<<<....<<<.<<.<......>....<<<.
.....>>>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<<<<<<.
...>>>>>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>...
....>>>>>....>>>.>>>.....>>>>>>>.....>.>>>>>...>>.
>>>>.>>>.....<<<<<<<......<<.....<<..<<<<....>>>>.
.>>....>>.......>>>>>>>......<....<<<<<<..........
>>>>>>....>.....>>>>>>....<<<<<<<<.......>>>>>>>>.
.....>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<
<<<<<.<<<..<.<<..<...<<<.>>>...>..>>.>..>>>.>>>>>>
>>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>>>>>>>>
.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6s47 A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling.
• Resolution: 3.28 Å
• Binding residue (original residue number in PDB): K96 K97 K99 A101 R118 F131 A133 H135 R138 Y140 G142 K143 H145 S146 K149
• Binding residue (residue number reindexed from 1): K1 K2 K4 A6 R23 F36 A38 H40 R43 Y45 G47 K48 H50 S51 K54

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0031386 protein tag activity
• GO:0031625 ubiquitin protein ligase binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002109 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0016567 protein ubiquitination
• GO:0019941 modification-dependent protein catabolic process
• GO:0042254 ribosome biogenesis
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6s47, PDBe:6s47, PDBj:6s47
• PDBsum: 6s47
• PubMed: 31299085
• UniProt: P05759|RS31_YEAST Ubiquitin-ribosomal protein eS31 fusion protein (Gene Name=RPS31)