Structure of PDB 7zrs Chain Bf Binding Site BS01

Receptor Information

>7zrs Chain Bf (length=112) Species: 4932 (Saccharomyces cerevisiae)

AQRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSAL
QGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIV
KKVVKEQTEAAK

Ligand information

>7zrs Chain 5 (length=3185)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacugggccagcaucaguuuugguggcaggauaaauccauaggaaug
uagcuugccucgguaaguauuauagccugugggaauacugccagcuggga
cugaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccg
cccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugg
guguaaaacccauacgcguaaugaaagugaacguagguuggggccucgca
agaggugcacaaucgaccgauccugaugucuucggauggauuugaguaag
agcauagcuguugggacccgaaagauggugaacuaugccugaauagggug
aagccagaggaaacucugguggaggcucguagcgguucugacgugcaaau
cgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagu
agcugguuccugccgaaguuucccucaggauagcagaagcucguaucagu
uuuaugagguaaagcgaaugauuagagguuccggggucgaaaugaccuug
accuauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaa
cguggacauuugaaugaagagcuuuuagugggccauuuuugguaagcaga
acuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacac
gcucaucagacaccacaaaagguguuaguucaucuagacagccggacggu
ggccauggaagucggaauccgcuaaggaguguguaacaacucaccggccg
aaugaacuagcccugaaaauggauggcgcucaagcguguuaccuauacuc
uaccgucaggguugauaugaugcccugacgaguaggcaggcguggagguc
agugacgaagccuagaccguaaggucgggucgaacggccucuagugcaga
ucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagug
gggaaagguuccacgucaacagcaguuggacguggguuagucgauccuaa
gagauggggaagcuccguuucaaaggccugauuuuaugcaggccaccauc
gaaagggaauccgguuaagauuccggaaccuggauauggauucuucacgg
uaacguaacugaauguggagacgucggcgcgagcccugggaggaguuauc
uuuucuucuuaacagcuuaucaccccggaauugguuuauccggagauggg
gucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugu
gacggcccgugaaaauccacaggaaggaauaguuuucaugccaggucgua
cugauaaccgcagcaggucuccaaggugaacagccucuaguugauagaau
aauguagauaagggaagucggcaaaauagauccguaacuucgggauaagg
auuggcucuaagggucggguacgaucaacuuagaacugguacggacaagg
ggaaucugacugucuaauuaaaacauagcauugcgauggucagaaaguga
uguugacgcaaugugauuucugcccagugcucugaaugucaaagugaaga
aauucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaag
guagccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaac
gagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggga
acgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacuc
uaguuugacauugugaagagacauagagggcuaccuuuauaguuucuuua
cuuauucaaugaagcggagcuggaauucauuuuccacguucuagcauuca
aggucccauucggggcugauccggguugaagacauugucagguggggagu
uuggcuggggcggcacaucuguuaaacgauaacgcagauguccuaagggg
ggcucauggagaacagaaaucuccaguagaacaaaaggguaaaagccccc
uugauuuugauuuucagugugaauacaaaccaugaaaguguggccuaucg
auccuuuagucccucggaauuugaggcuagaggugccagaaaaguuacca
cagggauaacuggcuuguggcagucaagcguucauagcgacauugcuuuu
ugauucuucgaugucggcucuuccuaucauaccgaagcagaauucgguaa
gcguuggauuguucacccacuaauagggaacgugagcuggguuuagaccg
ucgugagacagguuaguuuuacccuacugaugaauguuaccgcaauagua
auugaacuuaguacgagaggaacaguucauucggauaauugguuuuugcg
gcugucugaucaggcauugccgcgaagcuaccauccgcuggauuauggcu
gaacgccucuaagucagaauccaugcuagaacgcggugauuucuuugcuc
cacacaauauagauggauacgaauaaggcguccuuguggcgucgcugaac
cauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcuugc
uggcgaauugcaaugucauuuugcguggggauaaaucauuuguauacgac
uuagauguacaacgggguauuguaagcaguagaguagccuuguuguuacg
aucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.................
.........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<....
..<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>
>>>........<<<....>>>.....>>>>>>>.....>>>>>.>>>>>.
.<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<
<<.....>>>>...>>>...<<....>>.......<<<<<..<<<<<...
.>>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>...
.>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>
>>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>...>
..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<
<<<<<<.....<<<....<<<..<.<<<<...<<<<<.......>>>>>.
>>>>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>
>>.>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>
>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<
<<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<<..
..<<.........>>....>>.........<<......>>...>>>>>..
.>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>
>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<
.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>....
.>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<
<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...
<<....<<<....>>>........<<<.<<........>.>>>>....>>
....>>...<<<<<........>>>>>))<<<<.<<<<<.<.....<<<.
...>>>.......<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..{{{
..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>
>>.....>>>>>....><<<<<<<......>>>>>>>>>>>.>.>>>>>>
>.>>>>.........>>>>>>..........>.>..>>>>>>>>>>>>..
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
...<<<<<<<.<<.........<.....<<<<<....<<..>>.....>>
>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>............<
<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>
>......>>>>>>>...<<..<<<..<<<<<<.....<<.....<<<<..
..>>>>.....>>..>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<.......>>>>>.....>.....>>....>
>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<...
.....>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<
<.<<<<<<<<..<<<<<<<<<<<<<<<<<....>>>>>>>.>>>>>>>>>
>...<<<<<.....<<<<..<<<<<......>>>>>..............
.<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>
...<<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<
<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>
>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))....
...((((((..((((.>>>>>>))))))))))..........]]]]..}}
]]]].......<<.<.<.<<<<<<...<<.......>>...>>>>>>...
>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>.
..>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<<<
<<....>>>.>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<.
<.<<<<<<...............>>>>>>.><<<<<...<<<<....<<<
<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<
...........>>>>>...>>>>.>>>>....>>>>>>>>.......<<<
<<<.<<<.<<....<<..........<<<<<<.....>>>>>>......>
>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>
>>>>.............>>.>>>.>>>>>>..<<<<<.<...........
..>.....<<<<<<<<<.<<<....<<<<....<<<<<......>>>>>.
..>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zrs Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 4.8 Å
• Binding residue (original residue number in PDB): A2 R4 T6 F7 R8 R9 R10 N11 P12 Y13 N14 T15 R16 S17 K24 T25 P26 G27 R31 K36 K37 L38 T40 R41 P42 K43 G45 Q52 G53 I54 S55 T56 R58 P59 R60 Y62 A63 S66 K67 K70 T71 S73 R74 Y76 G78 A82 R88 R91 A92 I95 E96 Q98 K99 K102
• Binding residue (residue number reindexed from 1): A1 R3 T5 F6 R7 R8 R9 N10 P11 Y12 N13 T14 R15 S16 K23 T24 P25 G26 R30 K35 K36 L37 T39 R40 P41 K42 G44 Q51 G52 I53 S54 T55 R57 P58 R59 Y61 A62 S65 K66 K69 T70 S72 R73 Y75 G77 A81 R87 R90 A91 I94 E95 Q97 K98 K101

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zrs, PDBe:7zrs, PDBj:7zrs
• PDBsum: 7zrs
• PubMed: 36801861
• UniProt: P87262|RL34A_YEAST Large ribosomal subunit protein eL34A (Gene Name=RPL34A)