Structure of PDB 6uz7 Chain Bf Binding Site BS01

Receptor Information

>6uz7 Chain Bf (length=105) Species: 28985 (Kluyveromyces lactis)

AESHRLYVKGKHLSYQRSKSVNNPNVSLVKIEGVANPEDAKFYLGKRVAY
VYRCSKEVRGSKIRVIWGKINRTHGNSGVVRATFRSNLPAKTFGASVRIF
LYPSN

Ligand information

>6uz7 Chain 5 (length=3269)

uugaccucaaaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucuggcgucuucgacguccgaguuguaau
uugaagaaggcaacuuuguagcugguccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggaucccaguuauuugu
aaagugcuuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugggccagcauc
aguuuuagcgguuggauaaauccucgggaauguggcucugcuucgguaga
guguuauagcccgugggaauacagccagcugggacugaggauugcgacuu
uugucaaggaugcuggcguaaugguuaaaugccgcccgucuugaaacacg
gaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccguacg
cguaaugaaagugaacguaggugagggcccgcaagggugcaucaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
ugauuagagguaccgggguugaaaugaccuugaccuauucucaaacuuua
aauauguaagaaguccuuguugcuuaauugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguggaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacuccaccgucaggguuaauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuaggcu
guaaagcuggguagaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uucaaaggccuaauuuucuaggccaccaucgaaagggaauccgguuaaua
uuccggaaccuggauauggauucuucacgguaacguaacugaauguggag
acgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuau
caccccggaauugguuuauccggagagggggucuuauggcuggaagagcc
cagcccuugugcuggguccggugcgcccgcgacggcccuugaaaauccac
aggaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucu
ccaaggugaacagccucuaguugauagaauaauguagauaagggaagucg
gcaaaauagauccguaacuucgggauaaggauuggcucuaaggaucggaa
gugagggccuuggucagacgcggcguuguagacggccuugguaggucucu
uguagaucugcaauuaacgaucaacuuagaacugguacggacaaggggaa
ucugacugucuaauuaaaacauagcauugcgauggucagaaagugauguu
gacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaauu
caaccaagcgcggguaaacggcgggaguaacuaugacucucuuaagguag
ccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgaga
uucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacgg
gcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuagu
uugacauugugaagagacauagaggguguagcauaagugggagcuucggc
gccagugaaauaccacuaccuuuauaguuucuuuacuuauucaauuaagc
ggagcuggaauucauuuuccacguucuagcauucaaaguccuauacgggc
ugauccggguugaagacauugucagguggggaguuuggcuggggcggcac
aucuguuaaacgauaacgcagauguccuaagggggacucauggagaacag
aaaucuccaguagaacaaaaggguaaaaguccccuugauuuugauuuuca
gugugaauacaaaccaugaaaguguggccuaucgauccuuuagguccucg
gaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggcuu
guggcagucaagcguucauagcgacauugcuuuuugauucuucgaugucg
gcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguucac
ccacuaauagggaacgugagcuggguuuagaccgucgugagacagguuag
uuuuacccuacugaugaauguuaucgcaauaguaauugaacuuaguacga
gaggaacaguucauucggauaauugguuuuugcggcugucugaucaggca
uugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaaguca
gaauccaugcuagaacgcgaugauuucuuugccuugcacaauauagaagg
auaagaauaaggcgucuuuuuggcgucgcugaaccauagcaggcuggcaa
cggugcucuuagcggaaaggcuuugggugcuugccggcgaauugcaaugu
cauuuugcgcaaggauaaaucauuuguauacgacuuaaauguacaacagg
guauuguaagcaguagaguagccuuguuguuacgaucugcugagauuaag
ccuucguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<........<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<.<<<<......<<<<<<<....>>>>>
>>.......>>>>.>>.>.>.>>>>>.>>>>>>>>>>........<<<..
..>>>.....>>>>>>>.....>>>>>>>>>>>..<<<<........>>>
><<<<<<.....<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>
>...<<....>>.......<<<<<..<<<<....>>>>...>>>>>.<<<
<<<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>...<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<<<<<<<<<....<<.<<<<<<<.....<<<....
<<<....<<<<..<<<<<<<.....>>>>>>>>>>>....>>>..>.>>.
....>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>..
>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<.....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
......<<<<<<......>>>>>>....>>....>>...<<<<<......
..>>>>>))<<<<.<<<<<<<...<.<<<....>>>.......<<<<<<.
..<<<<.<<<<.<<.<<<<<<<<<<..{{{..>>>>>>....<<<<<...
.<<<<<.....<<<.....>>>.....>>>>>.....>>>>>.....<<<
<<<<......>>>>>>>>>>>...>>.>>>>.>>>>.........>>>>>
>.....>....>>>..>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.......
..<.....<<<<<...............>>>>><<.<<<<<<<<<<<...
..<...<<<..<<<<....<<<<..>>>>....>>>>..>>>........
.......>.........>>>>>..>>>>>>.>>............<<<<<
<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>...
...>>>>>>>...<<..<<<..<<<<<<....<<<....<<<<<....>>
>>.>...>>>.>>>>..>>>>><<<<<<.......>>>>>>........<
...........<<<<<.......>>>>>.....>.....>>....>>.>>
>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<.......
.>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<
<<<<<<..<<<<<..<.....<<<<..........<<<<<..<<....>>
............>>>>>.>>>>.....>...>>>>>...<<<<<.....<
<<<................................<..<.<.....>>..
>..>>>>..>>>>>.....>>>>>>>>..>>>>>....<<.....>>...
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<...
....>>>>.>>>>.<<<....>>>.>>))).......((((((..((((.
>>>>>>))))))))))..........]]]]..}}]]]].......<<.<.
<.<<<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<
<<..<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........
>>>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>..
..}}..}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<........
.......>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>
..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>
...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<<
..........<<<<<<.....>>>>>>......>>.......<<<<<<..
.<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>............
.>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<<<
<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>....
>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uz7 Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): A2 S4 H5 R6 Y8 K10 Q17 R18 S19 K20 S21 N23 P25 K42 L45 G46 R48 Y53 R54 K57 G61 S62 I64 R65 V66 W68 K70 R73 H75 N77 S78 R86 S87 N88 L89 P90 A91 K92 G95 A96 S97 R99
• Binding residue (residue number reindexed from 1): A1 S3 H4 R5 Y7 K9 Q16 R17 S18 K19 S20 N22 P24 K41 L44 G45 R47 Y52 R53 K56 G60 S61 I63 R64 V65 W67 K69 R72 H74 N76 S77 R85 S86 N87 L88 P89 A90 K91 G94 A95 S96 R98

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6uz7, PDBe:6uz7, PDBj:6uz7
• PDBsum: 6uz7
• PubMed: 31900355
• UniProt: Q6CRP7