Structure of PDB 6uz7 Chain Bc Binding Site BS01

Receptor Information

>6uz7 Chain Bc (length=97) Species: 28985 (Kluyveromyces lactis)

NINQKLALVVKSGKYSLGYKSTVKSLRQGKAKLIIIAANTPVLRKSELEY
YAMLSKTKVYYFQGGNNELGTAVGKLFRVGVVTILDAGDSDILTALA

Ligand information

>6uz7 Chain 5 (length=3269)

uugaccucaaaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucuggcgucuucgacguccgaguuguaau
uugaagaaggcaacuuuguagcugguccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggaucccaguuauuugu
aaagugcuuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugggccagcauc
aguuuuagcgguuggauaaauccucgggaauguggcucugcuucgguaga
guguuauagcccgugggaauacagccagcugggacugaggauugcgacuu
uugucaaggaugcuggcguaaugguuaaaugccgcccgucuugaaacacg
gaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccguacg
cguaaugaaagugaacguaggugagggcccgcaagggugcaucaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
ugauuagagguaccgggguugaaaugaccuugaccuauucucaaacuuua
aauauguaagaaguccuuguugcuuaauugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguggaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacuccaccgucaggguuaauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuaggcu
guaaagcuggguagaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uucaaaggccuaauuuucuaggccaccaucgaaagggaauccgguuaaua
uuccggaaccuggauauggauucuucacgguaacguaacugaauguggag
acgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuau
caccccggaauugguuuauccggagagggggucuuauggcuggaagagcc
cagcccuugugcuggguccggugcgcccgcgacggcccuugaaaauccac
aggaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucu
ccaaggugaacagccucuaguugauagaauaauguagauaagggaagucg
gcaaaauagauccguaacuucgggauaaggauuggcucuaaggaucggaa
gugagggccuuggucagacgcggcguuguagacggccuugguaggucucu
uguagaucugcaauuaacgaucaacuuagaacugguacggacaaggggaa
ucugacugucuaauuaaaacauagcauugcgauggucagaaagugauguu
gacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaauu
caaccaagcgcggguaaacggcgggaguaacuaugacucucuuaagguag
ccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgaga
uucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacgg
gcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuagu
uugacauugugaagagacauagaggguguagcauaagugggagcuucggc
gccagugaaauaccacuaccuuuauaguuucuuuacuuauucaauuaagc
ggagcuggaauucauuuuccacguucuagcauucaaaguccuauacgggc
ugauccggguugaagacauugucagguggggaguuuggcuggggcggcac
aucuguuaaacgauaacgcagauguccuaagggggacucauggagaacag
aaaucuccaguagaacaaaaggguaaaaguccccuugauuuugauuuuca
gugugaauacaaaccaugaaaguguggccuaucgauccuuuagguccucg
gaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggcuu
guggcagucaagcguucauagcgacauugcuuuuugauucuucgaugucg
gcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguucac
ccacuaauagggaacgugagcuggguuuagaccgucgugagacagguuag
uuuuacccuacugaugaauguuaucgcaauaguaauugaacuuaguacga
gaggaacaguucauucggauaauugguuuuugcggcugucugaucaggca
uugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaaguca
gaauccaugcuagaacgcgaugauuucuuugccuugcacaauauagaagg
auaagaauaaggcgucuuuuuggcgucgcugaaccauagcaggcuggcaa
cggugcucuuagcggaaaggcuuugggugcuugccggcgaauugcaaugu
cauuuugcgcaaggauaaaucauuuguauacgacuuaaauguacaacagg
guauuguaagcaguagaguagccuuguuguuacgaucugcugagauuaag
ccuucguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<........<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<.<<<<......<<<<<<<....>>>>>
>>.......>>>>.>>.>.>.>>>>>.>>>>>>>>>>........<<<..
..>>>.....>>>>>>>.....>>>>>>>>>>>..<<<<........>>>
><<<<<<.....<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>
>...<<....>>.......<<<<<..<<<<....>>>>...>>>>>.<<<
<<<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>...<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<<<<<<<<<....<<.<<<<<<<.....<<<....
<<<....<<<<..<<<<<<<.....>>>>>>>>>>>....>>>..>.>>.
....>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>..
>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<.....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
......<<<<<<......>>>>>>....>>....>>...<<<<<......
..>>>>>))<<<<.<<<<<<<...<.<<<....>>>.......<<<<<<.
..<<<<.<<<<.<<.<<<<<<<<<<..{{{..>>>>>>....<<<<<...
.<<<<<.....<<<.....>>>.....>>>>>.....>>>>>.....<<<
<<<<......>>>>>>>>>>>...>>.>>>>.>>>>.........>>>>>
>.....>....>>>..>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.......
..<.....<<<<<...............>>>>><<.<<<<<<<<<<<...
..<...<<<..<<<<....<<<<..>>>>....>>>>..>>>........
.......>.........>>>>>..>>>>>>.>>............<<<<<
<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>...
...>>>>>>>...<<..<<<..<<<<<<....<<<....<<<<<....>>
>>.>...>>>.>>>>..>>>>><<<<<<.......>>>>>>........<
...........<<<<<.......>>>>>.....>.....>>....>>.>>
>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<<.......
.>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<
<<<<<<..<<<<<..<.....<<<<..........<<<<<..<<....>>
............>>>>>.>>>>.....>...>>>>>...<<<<<.....<
<<<................................<..<.<.....>>..
>..>>>>..>>>>>.....>>>>>>>>..>>>>>....<<.....>>...
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<...
....>>>>.>>>>.<<<....>>>.>>))).......((((((..((((.
>>>>>>))))))))))..........]]]]..}}]]]].......<<.<.
<.<<<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<
<<..<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........
>>>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>..
..}}..}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<........
.......>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>
..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>
...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<<
..........<<<<<<.....>>>>>>......>>.......<<<<<<..
.<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>............
.>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<<<
<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>....
>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uz7 Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): L25 G26 Y27 K28 S29 P49 V50 R52 K53 S54 L84 F85 G88
• Binding residue (residue number reindexed from 1): L17 G18 Y19 K20 S21 P41 V42 R44 K45 S46 L76 F77 G80

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6uz7, PDBe:6uz7, PDBj:6uz7
• PDBsum: 6uz7
• PubMed: 31900355
• UniProt: P38664|RL30_KLULA Large ribosomal subunit protein eL30 (Gene Name=RPL30)