Structure of PDB 5ju8 Chain BV Binding Site BS01

Receptor Information

>5ju8 Chain BV (length=94) Species: 83333 (Escherichia coli K-12)

MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNM
QAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA

Ligand information

>5ju8 Chain BB (length=118)

gccuggcggccguagcgcgguggucccaccugaccccaugccgaacucag
aagugaaacgccguagcgccgaugguaguguggggucuccccaugcgaga
guagggaacugccaggca

<<<<<<<<<.....<<<<<<<<....<<<<<<<.............>>>>
..>>>...>>>>>>.>>.<<.......<<<<<<<<...>>>>>>>>....
...>>...>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5ju8 A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): R9 Q12 G13 K14 S17 R18 R19 R21 I29 Y31 Q75 Q78 H88
• Binding residue (residue number reindexed from 1): R9 Q12 G13 K14 S17 R18 R19 R21 I29 Y31 Q75 Q78 H88

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0009314 response to radiation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5ju8, PDBe:5ju8, PDBj:5ju8
• PDBsum: 5ju8
• PubMed: 27380950
• UniProt: P68919|RL25_ECOLI Large ribosomal subunit protein bL25 (Gene Name=rplY)