Structure of PDB 7zs5 Chain BU Binding Site BS01

Receptor Information

>7zs5 Chain BU (length=159) Species: 4932 (Saccharomyces cerevisiae)

GKSHGYRSRTRYMFQRDFRKHGAVHLSTYLKVYKVGDIVDIKANGSIQKG
MPHKFYQGKTGVVYNVTKSSVGVIINKMVGNRYLEKRLNLRVEHIKHSKC
RQEFLERVKANAAKRAEAKAQGVAVQLKRQPAQPRESRIVSTEGNVPQTL
APVPYETFI

Ligand information

>7zs5 Chain 1 (length=3248)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
aucucgcauuucacugggccagcaucaguuuugguggcaggauaaaucca
uaggaauguagcuugccucgguaaguauuauagccugugggaauacugcc
agcugggacugaggacugcgacguaagucaaggaugcuggcauaaugguu
auaugccgcccgucuugaaacacggaccaaggagucuaacgucuaugcga
guguuuggguguaaaacccauacgcguaaugaaagugaacguagguuggg
gccucgcaagaggugcacaaucgaccgauccugaugucuucggauggauu
ugaguaagagcauagcuguugggacccgaaagauggugaacuaugccuga
auagggugaagccagaggaaacucugguggaggcucguagcgguucugac
gugcaaaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaac
caucuaguagcugguuccugccgaaguuucccucaggauagcagaagcuc
guaucaguuuuaugagguaaagcgaaugauuagagguuccggggucgaaa
ugaccuugaccuauucucaaacuuuaaauauguaagaaguccuuguuacu
uaauugaacguggacauuugaaugaagagcuuuuagugggccauuuuugg
uaagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccg
gaauacacgcucaucagacaccacaaaagguguuaguucaucuagacagc
cggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuc
accggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuac
cuauacucuaccgucaggguugauaugaugcccugacgaguaggcaggcg
uggaggucagugacgaagccuagaccguaaggucgggucgaacggccucu
agugcagaucuuggugguaguagcaaauauucaaaugagaacuuugaaga
cugaaguggggaaagguuccacgucaacagcaguuggacguggguuaguc
gauccuaagagauggggaagcuccguuucaaaggccugauuuuaugcagg
ccaccaucgaaagggaauccgguuaagauuccggaaccuggauauggauu
cuucacgguaacguaacugaauguggagacgucggcgcgagcccugggag
gaguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccg
gagauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggu
gcgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcc
aggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguu
gauagaauaauguagauaagggaagucggcaaaauagauccguaacuucg
ggauaaggauuggcucuaagggucggguagugagggccuuggucagacgc
uuguuguagacggccuuggucuugcuacaauuaacgaucaacuuagaacu
gguacggacaaggggaaucugacugucuaauuaaaacauagcauugcgau
ggucagaaagugauguugacgcaaugugauuucugcccagugcucugaau
gucaaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgca
ugaauggauuaacgagauucccacugucccuaucuacuaucuagcgaaac
cacagccaagggaacgggcuuggcagaaucagcggggaaagaagacccug
uugagcuugacucuaguuugacauugugaagagacauagagggcuaccuu
uauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuuccac
guucuagcauucaaggucccauucggggcugauccggguugaagacauug
ucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacgcag
auguccuaaggggggcucauggagaacagaaaucuccaguagaacaaaag
gguaaaagcccccuugauuuugauuuucagugugaauacaaaccaugaaa
guguggccuaucgauccuuuagucccucggaauuugaggcuagaggugcc
agaaaaguuaccacagggauaacuggcuuguggcagucaagcguucauag
cgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccgaag
cagaauucgguaagcguuggauuguucacccacuaauagggaacgugagc
uggguuuagaccgucgugagacagguuaguuuuacccuacugaugaaugu
uaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggaua
auugguuuuugcggcugucugaucaggcauugccgcgaagcuaccauccg
cuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcggu
gauuucuuugcuccacacaauauagauggauacgaauaaggcguccuugu
ggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggc
cuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaauc
auuuguauacgacuuagauguacaacgggguauuguaagcaguagaguag
ccuuguuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<<....>>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.((......<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<....<<..
......>>....>>>..<<<<<<<<<<<<<<<<<<<<<<<.....<<<<<
<<<<......<<<<<<....>>>>>>.......>>>>>>>>..>.>>>>>
.>>>>>>>>>>........<<<....>>>....>>>>>>>>.....>>>>
>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..
<<<...<<<<.....>>>>...>>>...<<....>>.......<<<<<..
<<<<<....>>>>>...>>>>>.<<<<<<<<...<......>...>>>.>
>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<
<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.
.......>>>>.>>>....>>>>>>>>>...<<.......>>......>>
>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<
...<<<.<<<<<<<.....<<<....<<<....<<<<<..<<<<<<....
.>>>>>>>>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<.
.....>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.
....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.
....<<<<<<.....<<..........<<<.<<<<<<<<<........<<
<<<.<<<...<<.........>>...>>>.........<<......>>..
.>>>>>....>>>>>>>>>>>>..........))).>>...>>>>>>.>>
>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>>..
<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>
>........>>>>>><<.....<<.....<<<<<<.......>>>>>><<
<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<<<...<<....<<<....>>>........<<.<<<........>.>>
>>....>>....>>>..<<<<<........>>>>>))<<<<.<<<<<<<.
....<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<<<<<<<<<
<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>
....>>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.
>.>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>>>
>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>
..<<<<.....<<<<<<<.<<<........<.....<<<<<....<<..>
>.....>>>>><<.<<<<<<<<<<<.<<<<<<........<<<.....<.
......>.....>>>.......>>>>>>......>>>>>..>>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.....
<<.....<<<<....>>>>.....>>..>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<.......>>>>>.....
>.....>>....>>.>>>>>>>..>.>>>.>>>>>>>..>>>>>>....>
>....<<<<<........>>>>>....<<<<<..<<<.<<......<<<<
.<<<<<<..<<<<<.<<<<<<<<..<<.<<<<<<<<<<<<<......>>>
>>>.>>>>>>>.>>...<<<<<.....<<<<..<<<<<......>>>>>.
.........}}...<<<<<<....>>>>>>..>>>>..>>>>>.....>>
>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....
>>>.>>)))......<<<<<<<..<<<<.))))))>>>>>>>>>>>....
.....>>>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>
>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<.
.....>>>>>>>...>.<<<.........>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<
<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<.
..<<<<<...<<<<<<<<<<]]].>>>>>..>>>>>..>>>>>..>>>>>
.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>
>>>.......<<<<<<.<<<.<<....<<..........<<<<<<.....
>>>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>
>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<
.<.............>.....<<<<<<<<<.<<<....<<<<.<..<<<<
<......>>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zs5 Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): G2 K3 S4 H5 G6 Y7 R8 S9 R10 T11 R12 Y13 M14 Q16 R17 F19 H22 G23 S47 Q49 K50 G51 H54 K55 F56 Y57 Q58 G59 T61 T68 K69 S70 S71 K78 V80 R83 L85 K87 R88 N90 K100 C101 E104 F105 R108 V109 N112 Q127 L128 K129 R130
• Binding residue (residue number reindexed from 1): G1 K2 S3 H4 G5 Y6 R7 S8 R9 T10 R11 Y12 M13 Q15 R16 F18 H21 G22 S46 Q48 K49 G50 H53 K54 F55 Y56 Q57 G58 T60 T67 K68 S69 S70 K77 V79 R82 L84 K86 R87 N89 K99 C100 E103 F104 R107 V108 N111 Q126 L127 K128 R129

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zs5, PDBe:7zs5, PDBj:7zs5
• PDBsum: 7zs5
• PubMed: 36801861
• UniProt: Q02753|RL21A_YEAST Large ribosomal subunit protein eL21A (Gene Name=RPL21A)