Structure of PDB 7zs5 Chain BT Binding Site BS01

Receptor Information

>7zs5 Chain BT (length=172) Species: 4932 (Saccharomyces cerevisiae)

MAHFKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYFLQKLHK
VKKASGEIVSINQINEAHPTKVKNFGVWVRYDSRSGTHNMYKEIRDVSRV
AAVETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKF
PLPHRVQKSTKTFSYKRPSTFY

Ligand information

>7zs5 Chain 1 (length=3248)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
aucucgcauuucacugggccagcaucaguuuugguggcaggauaaaucca
uaggaauguagcuugccucgguaaguauuauagccugugggaauacugcc
agcugggacugaggacugcgacguaagucaaggaugcuggcauaaugguu
auaugccgcccgucuugaaacacggaccaaggagucuaacgucuaugcga
guguuuggguguaaaacccauacgcguaaugaaagugaacguagguuggg
gccucgcaagaggugcacaaucgaccgauccugaugucuucggauggauu
ugaguaagagcauagcuguugggacccgaaagauggugaacuaugccuga
auagggugaagccagaggaaacucugguggaggcucguagcgguucugac
gugcaaaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaac
caucuaguagcugguuccugccgaaguuucccucaggauagcagaagcuc
guaucaguuuuaugagguaaagcgaaugauuagagguuccggggucgaaa
ugaccuugaccuauucucaaacuuuaaauauguaagaaguccuuguuacu
uaauugaacguggacauuugaaugaagagcuuuuagugggccauuuuugg
uaagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccg
gaauacacgcucaucagacaccacaaaagguguuaguucaucuagacagc
cggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuc
accggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuac
cuauacucuaccgucaggguugauaugaugcccugacgaguaggcaggcg
uggaggucagugacgaagccuagaccguaaggucgggucgaacggccucu
agugcagaucuuggugguaguagcaaauauucaaaugagaacuuugaaga
cugaaguggggaaagguuccacgucaacagcaguuggacguggguuaguc
gauccuaagagauggggaagcuccguuucaaaggccugauuuuaugcagg
ccaccaucgaaagggaauccgguuaagauuccggaaccuggauauggauu
cuucacgguaacguaacugaauguggagacgucggcgcgagcccugggag
gaguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccg
gagauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggu
gcgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcc
aggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguu
gauagaauaauguagauaagggaagucggcaaaauagauccguaacuucg
ggauaaggauuggcucuaagggucggguagugagggccuuggucagacgc
uuguuguagacggccuuggucuugcuacaauuaacgaucaacuuagaacu
gguacggacaaggggaaucugacugucuaauuaaaacauagcauugcgau
ggucagaaagugauguugacgcaaugugauuucugcccagugcucugaau
gucaaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgca
ugaauggauuaacgagauucccacugucccuaucuacuaucuagcgaaac
cacagccaagggaacgggcuuggcagaaucagcggggaaagaagacccug
uugagcuugacucuaguuugacauugugaagagacauagagggcuaccuu
uauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuuccac
guucuagcauucaaggucccauucggggcugauccggguugaagacauug
ucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacgcag
auguccuaaggggggcucauggagaacagaaaucuccaguagaacaaaag
gguaaaagcccccuugauuuugauuuucagugugaauacaaaccaugaaa
guguggccuaucgauccuuuagucccucggaauuugaggcuagaggugcc
agaaaaguuaccacagggauaacuggcuuguggcagucaagcguucauag
cgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccgaag
cagaauucgguaagcguuggauuguucacccacuaauagggaacgugagc
uggguuuagaccgucgugagacagguuaguuuuacccuacugaugaaugu
uaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggaua
auugguuuuugcggcugucugaucaggcauugccgcgaagcuaccauccg
cuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcggu
gauuucuuugcuccacacaauauagauggauacgaauaaggcguccuugu
ggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggc
cuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaauc
auuuguauacgacuuagauguacaacgggguauuguaagcaguagaguag
ccuuguuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<<....>>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.((......<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<....<<..
......>>....>>>..<<<<<<<<<<<<<<<<<<<<<<<.....<<<<<
<<<<......<<<<<<....>>>>>>.......>>>>>>>>..>.>>>>>
.>>>>>>>>>>........<<<....>>>....>>>>>>>>.....>>>>
>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..
<<<...<<<<.....>>>>...>>>...<<....>>.......<<<<<..
<<<<<....>>>>>...>>>>>.<<<<<<<<...<......>...>>>.>
>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<
<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.
.......>>>>.>>>....>>>>>>>>>...<<.......>>......>>
>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<
...<<<.<<<<<<<.....<<<....<<<....<<<<<..<<<<<<....
.>>>>>>>>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<.
.....>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.
....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.
....<<<<<<.....<<..........<<<.<<<<<<<<<........<<
<<<.<<<...<<.........>>...>>>.........<<......>>..
.>>>>>....>>>>>>>>>>>>..........))).>>...>>>>>>.>>
>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>>..
<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>
>........>>>>>><<.....<<.....<<<<<<.......>>>>>><<
<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<<<...<<....<<<....>>>........<<.<<<........>.>>
>>....>>....>>>..<<<<<........>>>>>))<<<<.<<<<<<<.
....<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<<<<<<<<<
<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>
....>>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.
>.>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>>>
>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>
..<<<<.....<<<<<<<.<<<........<.....<<<<<....<<..>
>.....>>>>><<.<<<<<<<<<<<.<<<<<<........<<<.....<.
......>.....>>>.......>>>>>>......>>>>>..>>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.....
<<.....<<<<....>>>>.....>>..>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<.......>>>>>.....
>.....>>....>>.>>>>>>>..>.>>>.>>>>>>>..>>>>>>....>
>....<<<<<........>>>>>....<<<<<..<<<.<<......<<<<
.<<<<<<..<<<<<.<<<<<<<<..<<.<<<<<<<<<<<<<......>>>
>>>.>>>>>>>.>>...<<<<<.....<<<<..<<<<<......>>>>>.
.........}}...<<<<<<....>>>>>>..>>>>..>>>>>.....>>
>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....
>>>.>>)))......<<<<<<<..<<<<.))))))>>>>>>>>>>>....
.....>>>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>
>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<.
.....>>>>>>>...>.<<<.........>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<
<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<.
..<<<<<...<<<<<<<<<<]]].>>>>>..>>>>>..>>>>>..>>>>>
.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>
>>>.......<<<<<<.<<<.<<....<<..........<<<<<<.....
>>>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>
>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<
.<.............>.....<<<<<<<<<.<<<....<<<<.<..<<<<
<......>>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zs5 Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): M1 N62 I64 N65 E66 A67 H68 P69 K71 R84 H88 N89 M90 Y91 Q108 A111 A112 R113 H114 R115 R117 T132 R137 Y139 L144 K146 H154 R155 Q157 T160 K161 Y165 K166 P168 S169 T170 F171
• Binding residue (residue number reindexed from 1): M1 N62 I64 N65 E66 A67 H68 P69 K71 R84 H88 N89 M90 Y91 Q108 A111 A112 R113 H114 R115 R117 T132 R137 Y139 L144 K146 H154 R155 Q157 T160 K161 Y165 K166 P168 S169 T170 F171

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zs5, PDBe:7zs5, PDBj:7zs5
• PDBsum: 7zs5
• PubMed: 36801861
• UniProt: P0CX23|RL20A_YEAST Large ribosomal subunit protein eL20A (Gene Name=RPL20A)