Structure of PDB 6skg Chain BR Binding Site BS01

Receptor Information

>6skg Chain BR (length=120) Species: 311400 (Thermococcus kodakarensis)

VKRTGPTDINLRRLIRTLRKKSNEEGVKIWKDIAWRLERPRRQRAEVNIS
KINRYTKEGDVVIVPGSVLGAGRLEHKVVVAAWKFSEAARRKIIEAGGEA
ISIEELMERNPKGSGVIIME

Ligand information

>6skg Chain BA (length=2934)

cccgguggcaacuaagccgccugguggauggcucggcucggggcgccgac
gaagggcguggcaagcugcgauaagccccggcgaggcgcaggcagccguc
gaaccggggaugcccgaaugggaccucccgcggcuugccgcacucccagu
cgggagggggaacgcggggaauugaaacaucuuaguacccgcaggaaaag
aaagcaaaagcgaugccguuaguaggggcgaccgaaagcggcacagggca
aacugaaccccgggccgaaagguucgggggaugugguguuguagggcccg
cggaggagcccugggggcgaagccgaaguccgcuggaacgcggcgccgga
gagggugaaagccccguaggcguaagcccccgagcuccugcggugucccu
gaguaccgucggucggauauccggcgggaagcugggaggcaucggcuccc
aacccuaaauacgucccgagaccgauagcgaacuaguaccgugagggaaa
gcugaaaagcaccccgggaggggggugaaaagagccugaaaccaggcggc
gauaggagggugcggcccgaaaggguugacccuccccgaaggaaacacgg
gcgaccguggaguacgaggggaggugaccgggguugcaccguccgucuug
gaucacggggcagggaguucacccgagcggcgagguuaagggggucaacc
ccgaagccgcagggaaaccgacagguccgcagcccguagggcgagggacg
gggugugaaagcgcccggagucgcucgggugagacccgaagccggucgau
cuagcccggggcagggugaagucccucaacagagggauggaggcccgcua
ggggugcuggcgugcaauucgcucccgugaccccgggcuaggggugaaag
gccaaucgaggccggcgauagcugguucccgccgaauuaucccccaggau
agcccggccggagguaggcagugggguagagcacugauuggggguuuagg
gggcgaaagccuccggcucccugucaaacuccgaacccacugccgccgua
gauggccggaguaggggggcgguguaagccgucacccgagaggggaacaa
cccagaccgggguuaaggccccaaagugccggcuaaguguuacuccaaag
ggugucccuggccuuagacagcggcccgucgaggucaggggccccgaaaa
uggacggggcuuaagccggccgccgagaccccggcgcacggaccgauugg
uccgugaucggguaggcgggcgugccgauggggcggaagccgggccguga
gguccgguggacccgucgguauugcggauccugccgggaguagcagcaua
gccgggugagaaucccggccgccguaggggccaggguuccacggcaaugu
ucgucagccguggguuagucgguccuaacccgacccguaacucggcgucg
gggaaagggaaacggguuuauauucccguaccgcggugguaggugcggca
acgcaagcccgaggggugacgccucgggguaggcggaccgguccacaagg
ccggcuaagcguauaagcccggggagugccguaauggcgagaaccgggug
aaagcgcgaauggccucccguaagggggguuccgccgaucccuggggccc
gugaaaagccccucgggaacgauccaccgcgaccguaccgagaaccgaca
cuggugccccugggugagaagccuaaggcgugucgggggaaacccagccg
agggaacucggcaaauuggccccguaacuucgggagaaggggugccugcg
gguguguaacccgcaggucgcagugacuaggggggcccgacuguuuagua
aaaacacaggucccagcuagcccgaaaggguuuguacuggggccgacgcc
ugcccagugccgguaugugaagcccggguacaaccgggugaagcaccggu
aaacggcggggguaacuauaacccucuuaagguagcgaaauuccuugucg
guuaaaugccgaccugcaugaauggcguaacgagguccccacuguccccg
gcuggggcccggcgaaaccucugccaggcgcauaugccugggaccuccgg
ugggaagcgaagaccccauggagcuuuacugcagccugccguugccguac
ggcggggggugcgcagcguaggcgggaggcgucgaagcccgcccuccggg
gcggguggagccgcccaugagacaccgcccacccucugccguacggcuaa
cccgcggaagcggggacagcgguaggugggcaguuuggcuggggcggcac
acccucgaaaagguaucgagggugcccuaaggucggcucaggcgggucag
gaauccgccguagagugcaagggcaaaagccggccugacuggacccguaa
cagaggcggguccagccgcgaaagcguggccuagcgaaccccugugccuc
cccggugggggccagggaugacagaaaagcuacccuggggauaacagagu
cgucucgggcgagagcccauaucgaccccgaggcuugcuaccucgcuguc
ggcucuucccauccuggcccugcagcaggggccaaggguggggguguuca
cccauuaaaggggaacgugagcugggcuuagaccgucgugagacaggucg
gaugcuaucuaccggagguguuggccgccugaggggaaggcucccccagu
acgagaggaacagggagccgcggccucuggucuaccgguuguccuacagg
gcauagccgggcagcuacgccgcagccgauaaggccugaaagcaucuaag
gccgaagcggcccccgaaaacaggcggccaccgagggcccgggaagaaga
cccgguugauggggcggggauguaagcgggaagggaaaccgacccguuca
gucugccgcucccaacagcccgagugcuaccggg

<<<<<<<<<<.....<<<<<<<<<<....[<<..<<<<<<<.[<<<<...
...<<<<<<.((..((.>>>.....<<<<<<...<<<))..)).>>>...
...>>>>>>...>>>....<<<....>>><<<<<..>>>>>.<<<<<...
.>>>>>.>>....<<<<<.....<<....>>......>>>>>........
...<<....>>..<<<<<<.....<<.....>>....>>>>>>..<<<..
.<<<...<<<<<<<<<....>>>>>>>>>.....>>>.....<<<<.<<<
<..<<<<<<..<<<<<<<...<<<.....<<<<......>>>>.((....
..<<<.....))>>>...>>>....>>>>>>>.>>>>>>>>>>...>>>>
.....<<<<<<<........>>>>>>>......<<<<<.<<<<<<>>>>>
..>>>......>>.]>>>>>.>>...............<<....>>....
>>.........<<<<....>>>>............]....>>>>>>>>>>
........<<<<<<<<<<....<<......<<<<<<<...<....<<<<<
....>>>>>.....>..>>>>>>>...>>.>>>>>>>>>>..<<<<....
....>>>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<.....>>
>>...>>>...<<....>>.....<<<<...<<<<...>>>>...>>>>.
<<<<<<....>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>....
<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<<<.....
>>>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>
><<<<<<<<<..<<<.<<<<<<<<<...<<<...<<<<.<<<<<<....<
<<<.......>>>>.>>>>>>.>>>>..>>.>...>>>>>>>>>>>>...
..>>>>>>>.>>..<<<<<<<<<....>>>>>>.>>>....<<<......
>>>...<<<<<<<...<<<(((.....<<<<<<.....<<.........<
<<.<<<<<<<<<<.........<..>......>>>>>>>>>>>>>.....
.....)))>>....>>>>>>.>>>...>>>>>>><<<<<<<<<<....>>
>>>>>>..>>....>>>>>><.<<<<<<<<<<......<<<<<<<<....
>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<......
<<<<<<.......>>>>>><<<<....<<<...<<...<<<<<<...<<.
....>>.>>>>>>....((<<<<<<...<<<<<<<<<.....>>>.>>>>
>>....>>...<<<<<........>>>>>))<<<<<<<<.....<<<...
.>>>...<<<<<<<<<<...<<<<<<<<<..<<<<<<<<<<<<.....>>
>>>>....<<<<.<..<<<<<<.....<<<<...>>>>.....>>>>>>.
..>>>>>.....<<<<<<......>>>>>>>>>>>>...>>>>>>>>>..
.......>>>>>>>>>>......>>>>>>>>>>>>..>>.....<<....
..>>...>>>.<<<<.....>>>>..>>>>...<<<<.....<<<<<<<<
.<<.........<.....<<<<<....<<..>>.....>>>>><<<<<<<
<<<.....>>>>>>>>>>............<<<<<<<<<..<<<......
...>>>..<<<<<<<....<<<<....>>>>......>>>>>>>.<<<<<
.<<<..<<<<<<<..<<<...<<<<<<......>>>>>>...>>>>>>>>
..>>>>><<<..............>>>.......<...........<<<<
<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>
>>>>>>.>>>>>>....>>....<<<<<........>>>>>..<<<<<<<
<.<<<.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<<<<
<<<<.<<<<<..........<<<<<..<<<......<<<<<.<<.....>
>>>>>>...>>>............>>>>>.>>>>>.>>>>>>>>>>>...
<<<<<....>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<...(((>>....>>>>>>>...<<<<<<<<<<..
.....>>>>>>>>>>.<<<....>>>.>>))).......<<<<<.<<<<<
.>>>>>>>>>>>>>>>>..........>>>>..]]>>>>.......<<<<
.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<
<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>..<<<.......
>>>......>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.
...]]..>>>>>>>>>>>....<<<<<<<<..<<<<..<<<<<<<.....
............>>>>>>><<<<<...<<<<...<<<<<<<<<<....>>
>>>..>>>>>...>>>>.>>>>>.<<<<.<..<<<<<...........>>
>>>...>>>>>>>>>>......>>>>>>>.....<<<<<<<<<.......
>>>>>....<<<<<<<<<<<<....<<<<<..<<....>>..>>>>>...
>>>>.>>>>>.>>>...>>>>...>>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6skg Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping.
• Resolution: 2.65 Å
• Binding residue (original residue number in PDB): K3 R4 T5 G6 P7 T8 K21 N24 D33 R42 R43 Q44 E47 S51 K52 R55 Y56 L70 G71 A72 G73 K85 S87 S115 G116 M120 E121
• Binding residue (residue number reindexed from 1): K2 R3 T4 G5 P6 T7 K20 N23 D32 R41 R42 Q43 E46 S50 K51 R54 Y55 L69 G70 A71 G72 K84 S86 S114 G115 M119 E120

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0043232 intracellular non-membrane-bounded organelle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6skg, PDBe:6skg, PDBj:6skg
• PDBsum: 6skg
• PubMed: 32555463
• UniProt: Q5JJF5|RL18E_THEKO Large ribosomal subunit protein eL18 (Gene Name=rpl18e)