Structure of PDB 6s47 Chain BR Binding Site BS01

Receptor Information

>6s47 Chain BR (length=141) Species: 4932 (Saccharomyces cerevisiae)

AVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPL
LLVGLDKFSNIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSK
NELKKAFTSYDRTLLIADSRRPEPKKFGGKGARSRFQKSYR

Ligand information

>6s47 Chain BA (length=1707)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccaacggggccuuuccuucuggccaggacuuuuacuuugaaaaaauua
gaguguucaaagcaggcguauugcucgaauauauuagcauggaauaauag
aauaggacguuugguucuauuuuguugguuucuaggaccaucguaaugau
uaauagggacggucgggggcaucaguauucaauugucagaggugaaauuc
uuggauuuauugaagacuaacuacugcgaaagcauuugccaaggacguuu
ucauuaaucaagaacgaaaguuaggggaucgaagaugaucagauaccguc
guagucuuaaccauaaacuaugccgacuagggaucgggugguguuuuuuu
aaugacccacucggcaccuuacgagaaaucaaagucuuuggguucugggg
ggaguauggucgcaaggcugaaacuuaaaggaauugacggaagggcacca
ccaggaguggagccugcggcuuaauuugacucaacacggggaaacucacc
agguccagacacaauaaggauugacagauugagagcucuuucuugauuuu
gugggugguggugcauggccguucuuaguugguggagugauuugucugcu
uaauugcgauaacgaacgagaccuuaaccuacuaaauaguggugcuagca
uuugcugguuauccacuucuuagagggacuaucgguuucaagccgaugga
aguuugaggcaauaacaggucugugaugcccuuagacguucugggccgca
cgcgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucu
ugguaaucuugugaaacuccgucgugcuggggauagagcauuguaauuau
ugcucuucaacgaggaauuccuaguaagcgcaagucaucagcuugcguug
auuacgucccugcccuuuguacacaccgcccgucgcuaguaccgauugaa
uggcuuagugaggccucaggaucugggagaauuuggacaaacuuggucau
uuagaggaacuaaaagucguaacaagguuuccguaggugaaccugcggaa
ggaucau

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[..................<.....<<
.<<<.....>>>.>>......>.........<<<<<..<<....>>..>>
>>>[...((((((......<<<<<<<<<<......>>....<<......>
>)))))).....]...<<<<..<<<.....>>>.>>>>....]]]...}}
}}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<.......
...>>>.>>.<.<<....>>.>...]]]]]].........<<<....<<<
.....>>>..>>>...............<<.<<....<..........>.
...>>..>>......<<..<...........>..>>.........<<<<<
((....<.<<<<.....<<..))>>.......>>>>>>>>>>..))))))
]]]].........[[[{{.......{{...[[[.[[....<<<<<<.<<<
<..>>>>.>>>>>>....<<<<<..>>>>>...<<<<<.<<.......<<
...<.......>..<<<.....>>>....>>......>>.>>..>>>...
.......[[..((((((((....>>>>>>>>.)))))))).]]......]
]....<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>
......>.>>>>>>..>>.......<<....>>...>>>>...>>>>>.>
>>...]]]...}}......<<<<<<<...<...<<<<.<.....>.>>>>
...>>>>>>>>..........<<<.<<.<<<..<<<<<<<<.<<<<....
.>.>>>>>>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<
<......<<<<....>>>>....>>>..]]]]..<<<<<.<<<<<<<..<
<.<<<<<<..<<<.<<<<<<......<<........>>..........<<
<<<.<....<<<<<........<<.<<<........>>>.>>......>>
>>>...<<.<<<..<<.<<<<<<....<<<.<<.<......>....<<<.
.....>>>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<<<<<<.
...>>>>>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>...
....>>>>>....>>>.>>>.....>>>>>>>.....>.>>>>>...>>.
>>>>.>>>.....<<<<<<<......<<.....<<..<<<<....>>>>.
.>>....>>.......>>>>>>>......<....<<<<<<..........
>>>>>>....>.....>>>>>>....<<<<<<<<.......>>>>>>>>.
.....>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<
<<<<<.<<<..<.<<..<...<<<.>>>...>..>>.>..>>>.>>>>>>
>>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>>>>>>>>
.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6s47 A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling.
• Resolution: 3.28 Å
• Binding residue (original residue number in PDB): Q8 F10 K12 K14 S15 V19 H21 K30 E40 R66 G71 G72 G73 H74 V75 S76 R122 R123 P124 E125 P126 K127 K128 F129 G130 R135 S136 R137 F138 Q139 K140 S141 Y142 R143
• Binding residue (residue number reindexed from 1): Q6 F8 K10 K12 S13 V17 H19 K28 E38 R64 G69 G70 G71 H72 V73 S74 R120 R121 P122 E123 P124 K125 K126 F127 G128 R133 S134 R135 F136 Q137 K138 S139 Y140 R141

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation
• GO:0006413 translational initiation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6s47, PDBe:6s47, PDBj:6s47
• PDBsum: 6s47
• PubMed: 31299085
• UniProt: P0CX51|RS16A_YEAST Small ribosomal subunit protein uS9A (Gene Name=RPS16A)