Structure of PDB 6olg Chain BO Binding Site BS01

Receptor Information

>6olg Chain BO (length=136) Species: 9606 (Homo sapiens)

SLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKAD
RDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSA
LRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL

Ligand information

>6olg Chain B1 (length=1708)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgacuuggauaacugugguaauucua
gagcuaauacaugccgacgggcgcugacccccuucgcgggggggaugcgu
gcauuuaucagaucaaaaccaacccgggcuuuggugacucuagauaaccu
cgggccgaucgcacgccccccguggcggcgacgacccauucgaacgucug
cccuaucaacuuucgaugguagucgccgugccuaccauggugaccacggg
ugacggggaaucaggguucgauuccggagagggagccugagaaacggcua
ccacauccaaggaaggcagcaggcgcgcaaauuacccacucccgacccgg
ggagguagugacgaaaaauaacaauacaggacucuuucgaggcccuguaa
uuggaaugaguccacuuuaaauccuuuaacgaggauccauuggagggcaa
gucuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaa
guugcugcaguuaaaaagcucguaguuggaucuugggagcggccccuugc
cucucgcccgaagcguuuacuuugaaaaaauuagaguguucaaagcaggc
ccgagccgccuggauaccgcagcuaggaauaauggaauaggaccgcgguu
cuauuuuguugguuuucggaacugaggccaugauuaagagggacggccgg
gggcauucguauugcgccgcuagaggugaaauucuuggaccggcgcaaga
cggaccagagcgaaagcauuugccaagaauguuuucauuaaucaagaacg
aaagucggagguucgaagacgaucagauaccgucguaguuccgaccauaa
acgaugccgaccggcgaugcggcggcguuauucccaugacccgccgggca
gcuuccgggaaaccaaagucuuuggguuccggggggaguaugguugcaaa
gcugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccug
cggcuuaauuugacucaacacgggaaaccucacccggcccggacacggac
aggauugacagauugauagcucuuucucgauuccgugggugguggugcau
ggccguucuuaguugguggagcgauuugucugguuaauuccgauaacgaa
cgagacucuggcaugcuaacuaguuacgcgacccccgagcgguaacuucu
uagagggacaaguggcguucagccacccgagauugagcaauaacaggucu
gugaugcccuuagauguccggggcugcacgcgcgcuacacugacuggcuc
agcgugugccuacccuacgccggcaggcgcggguaacccguugaacccca
uucgugauggggaucggggauugcaauuauuccccaugaacgaggaauuc
ccaguaagugcgggucauaagcuugcguugauuaagucccugcccuuugu
acacaccgcccgucgcuacuaccgauuggaugguuuagugaggcccucgg
aucggccccgccguggcggagcgcugagaagacggucgaacuugacuauc
uagaggaaguaaaagucguaacaagguuuccguaggugaaccugcggaag
gaucauua

...<<<<<...[[[[>>>>>[[[.((((((.....{{.........[[[.
[[[..<<....<<....<<..........>>...>>.>>......{{...
.....[[[......{{....[[[[[....<<.....<<.<<<.....>>>
.>>......>>.........<<<<...<<<<<<....>>>>>>...>>>>
[[..(((((.....<<<<<<<<<<<.>>....<......>..)))))...
...]]...<<<<.<<<<......>>>>>>>>.]].]]]...}}....]]]
.<<<....<<<....<<<<<<<.........>>>>>>>>>>......>>>
...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>.
>>.<.<<....>>.>...]]]]]].........<<<....<<<.....>>
>..>>>......}}.......<<<<<<<<<.<<<....>>>..>>>>>.>
>>>......<<..<...........>..>>.........<<<<<((....
..<<<<.....<<..))>>.......>>>>.>>>>>..)))))).]]]..
..........{{.......{{...[[[.......<<<.....>>>....<
.<..<....>>.>...<<<<<<.<.......<<...<.......>.<<<<
.......>>>>...>>......>.>>>..>>>........[....(((((
((.....>>>>>>>>>.)))))))....]..........<<<<<<..<..
.<<<<..<<..<<<<<<<<...<<<......>>>......>>>>>>>>..
>>.......<<....>>...>>>>..>..>>>.>>>...]]]...}}...
...<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>>>.....
.....<<<.<<.<<<..<.<<<<<<.<<<........>>>>>>>>>.>..
>>>...<<..}}>>...>>.....>>>.....<<<......<<<<....>
>>>....>>>..]]]]..<<<<<.<<<<<<<..<<..<<<<<..<<<.<<
<<<.......<<........>>..........<<<<<.<....<<<<<..
......<<.<<<........>>>.>>......>>>>>...<<.<<<..<<
.<<<<<<....<<<.<<<<......>>...<<<......>>>...>>.>>
>....<<<...<...<<<<..<<<<..<<..<......>..>>>>>>..>
>>>..>.....<<<<<.....>>>>>........>>>....>>>.>>>..
...>>>>>>>.....>.>>>>>....>.>>>>.>>>.....<<.<<<...
.....<.......<<<.<<<<....>>>>.>>>....>.........>>>
.>>......<...<.<<<<<..........>>>>>>....>.....>>>>
>.....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>
.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<<<.<<..
.<<<<<.<<<<<..>>>>>...>>>>>...>>.>>>>..>>>.>>>>>>>
>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>>>>>>>>.
........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6olg Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): N38 D39 F41 G49 K50 T52 R55 T57 G59 M60 D65 R66 E68 R98 R104 I135 P136 S137 D138 R141 R142 K143 R146 R147 R149 R150 L151
• Binding residue (residue number reindexed from 1): N23 D24 F26 G34 K35 T37 R40 T42 G44 M45 D50 R51 E53 R83 R89 I120 P121 S122 D123 R126 R127 K128 R131 R132 R134 R135 L136

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0045182 translation regulator activity
• GO:0048027 mRNA 5'-UTR binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0000122 negative regulation of transcription by RNA polymerase II
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006417 regulation of translation
• GO:0030218 erythrocyte differentiation
• GO:0030490 maturation of SSU-rRNA
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0014069 postsynaptic density
• GO:0015935 small ribosomal subunit
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0045202 synapse
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6olg, PDBe:6olg, PDBj:6olg
• PDBsum: 6olg
• PubMed: 31160784
• UniProt: P62263|RS14_HUMAN Small ribosomal subunit protein uS11 (Gene Name=RPS14)