Structure of PDB 8c3a Chain BK Binding Site BS01

Receptor Information

>8c3a Chain BK (length=176) Species: 5476 (Candida albicans)

VRYAATPANPAKSASARGSYLRVSFKNTRETAQAINGWKLEKAQKYLDQV
LDHQRAIPFRRYNSSIGRTGQGKEFGVTKARWPAKSVNFVKDLLRNAQAN
AEAKGLDSSKLVISHIQVNHAPKQRRRTYRAHGRINAYQSTPSHIELTLT
EEDEKQIRLNSRQRGRLASQKRLTAA

Ligand information

>8c3a Chain AS (length=3229)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuugca
ugccgcugcgguuuaccgggccagcaucgguuuggagcggcaggauaaug
gcggaggaauguggcacggcuucugcuguguguuauagccucugacgaua
cugccagccuagaccgaggacugcgguuuuuaccuaggauguuggcauaa
ugaucuuaagucgcccgucuugaaacacggaccaaggagucuaacgucua
ugcgaguguuuggguguaaaacccguacgcguaaugaaagugaacgaagg
ugggggcccauuagggugcaccaucgaccgauccugauguguucggaugg
auuugaguaagagcauagcuguugggacccgaaagauggugaacuaugcc
ugaauagggugaagccagaggaaacucugguggaggcucguagcgguucu
gacgugcaaaucgaucgucgaauuuggguauaggggcgaaagacuaaucg
aaccaucuaguagcugguuccugccgaaguuucccucaggauagcagaag
cucguaucaguuuuaugagguaaagcgaaugauuagaagucuugggguug
aaaugaccuuaacuuauucucaaacuuuaaauauguaagaaguccuuguu
gcuuaauugaacguggacaauugaaugaagagcuuuuagugggccauuuu
ugguaagcagaacuggcgaugcgggaugaaccgaacgugaaguuaaagug
ccggaaugcacgcucaucagacaccacaaaagguguuaguucaucuagac
agccggacgguggccauggaagucggaauccgcuaaggaguguguaacaa
cucaccggccgaaugaacuagcccugaaaauggauggcgcucaagcgugc
uacuuauacuucaccgugauugcuguuuugacgcuuucacgaguaggcag
gcguggaggucagugacgaagccuuugcuguaaagcugggucgaacggcc
ucuagugcagaucuuggugguaguagcaaauauucaaaugagaacuuuga
agacugaaguggggaaagguuccaugucaacagcaguuggacauggguua
gucgauccuaagagauggggaagcuccguuucaacgugcuugauuuuuca
ggccaaccaucgaaagggaauccgguuaaaauuccggaacuuggauaugg
auucuucacggcaacguaacugaauguggagacgucggcgugagcccugg
gaggaguuaucuuuucuucuuaacagcuuaucacccuggaauugguuuau
ccggagauggggucuuauggcuggaagagcgcgguaauuuugccgcgucc
ggugcgcuuacgacgguccuugaaaauccacaggaaggaauaguuuucau
gccaagucguacucauaaccgcagcaggucuccaagguuaacagccucua
guugauagaauaauguagauaagggaagucggcaaaauagauccguaacu
ucgggauaaggauuggcucuaaggaucgggcacguuuaacgaucaacuua
gaacugguacggacaaggggaaucugacugucuaauuaaaacauagcauu
gugauggucagaaagugauguugacacaaugugauuucugcccagugcuc
ugaaugucaaagugaagaaauucaaccaagcgcggguaaacggcgggagu
aacuaugacucucuuaagguagccaaaugccucgucaucuaauuagugac
gcgcaugaauggauuaacgagauucccacugucccuaucuacuaucuagc
gaaaccacagccaagggaacgggcuuggcagaaucagcggggaaagaaga
cccuguugagcuugacucuaguuugacauugugaaaagacauggagggug
uagaauaagugggagcuucggcgccggugaaauaccacuaccucuauagu
uuuuuuacuuauucaaugaagcggagcuggaggucaaacuccacguucua
gcauuaagcccucugggcgauccggguugaagacauugucagguggggag
uuuggcuggggcggcacaucuguuaaacgauaacgcagguguccuaaggg
ggacucauggagaacagaaaucuccaguagaacaaaaggguaaaaguccc
cuugauuuugauuuucagugugaauacaaaccaugaaaguguggccuauc
gauccuuuagucccucggaauuugaggcuagaggugccagaaaaguuacc
acagggauaacuggcuuguggcagucaagcguucauagcgacauugcuuu
uugauucuucgaugucggcucuuccuaucauaccgaagcagaauucggua
agcguuggauuguucacccacuaauagggaacgugagcuggguuuagacc
gucgugagacagguuaguuuuacccuacugaugaauguuaucgcaauagu
aauugaacuuaguacgagaggaaccguucauucagauaauugguuuuugc
ggcugucugaucaggcaacgccgcgaagcuaccaucugcuggauuauggc
ugaacgccucuaagucagaauccaugcuagaacgcgaugauuuuugcccu
gcacauuuuagauggauacgaauaagacuuuuuagucgcuggaccauagc
aggcuggcaacggugcgcuuagcggaaaggcuuugugcgcuugccggcgg
auagcaaugucaacaugcgcggggauaaauccuuugcauacgacuuagau
guacaacggaguauuguaagcaguagaguagccuuguuguuacgaucugc
ugagauuaagcucuuguugucugauuugu

.......................<<<<<<.....<......>......>>
>>>.>...<.....<<....>>..<<<<<......<<.....>>.....>
>>>>..<<<...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<.....<<<<......>>>
>.(.......<<<......)>>>....>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<...<<<<
......>>>>...>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.
<<<<<<<......<<<<<<......>>>>>>.......>>>>>>.>.>>.
>>>>>.>>>>>>>>>>........<<<....>>>.....>>>>>>>....
>>>>>>>>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<
<<<..<<<..<<<<<.....>>>>>..>>>...<<....>>.......<<
<<<..<<<<....>>>>...>>>>>.<<<<<<<<...<<....>>...>>
>.>>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<
<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.
<<........>>>>.>>>....>>>>>>>>>...<<.......>>.....
.>>>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<
<<<...<<<.<<<<<<<.....<<<....<<<....<<<<...<<<<<..
.....>>>>>.>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<
.<......>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<
<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(
((.....<<<<<<.....<<..........<<<.<<<<<<<<<<......
.<<<<<.<<<...<<.........>>...>>>.........<<......>
>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>
.>>>>..>>>>>>><<<<<..<<.........>>..>>>>>....>>>>>
>..<<<<<<<<.<<......<<<<..<<<....>>>.>>>>...>>>>>>
>>>>........>>>>>><<.....<<.....<<<<<<.......>>>>>
><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<<<...<<....<<<....>>>..........<<<<<<....>>>
>>>......>>....>>...<<<<<........>>>>>))<<<<.<<<<<
<<.....<<<....>>>.....{{<<<<<<...<<<<.<<<<<<<.<<<<
<<<<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....
>>>....>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>
>>...>>>>>>>.>>>>.........>>>>>>..........>>>..>>>
>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>
>>>..<<<<.....<<<<<<<.<<.........<.....<<<<<......
.........>>>>><<.<<<<<<<<<<<...........>>>>>..>>>>
>>.>>............<<<<<<<<<..<<<.........>>>..<<<<<
<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<
....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<
.......>>>>>>........<...........<<<<<.......>>>>>
.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>
....>>....<<<<<........>>>>>....<<<<<..<<<.<<.....
.<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<<<<...<<...
..............<......>...................>>..>>.>>
>>>>>>>>...<<<<<.....<<<<..<<<<<......>>>>>.......
...}}..<<<<...>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>
>...<<<.....>>>..<<<<<<...........>>>>>>..<<...<<<
<<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>
>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))...
...<<<<<<<..<<<<.))))))>>>>>>>>>>>.........>>>>..>
>>>>>.......<<.<.<.<<<<<<...<<.......>>...>>>>>>..
.>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>
...>.<<<.........>>>.....>>>>>..>...>>>.>>....<<<<
<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<<<..<<<<
...<<<<<.................>>>>>..<<<<<...<<<<....<<
<<<<<<<<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<
<...........>>>>>...>>>>.>>>>....>>>>>>>>.....<<<<
<<.<<<.......<<..........<<<<....>>>>......>>.....
..<<<<<<...<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>..
..............>>>.>>>>>>..<<<<<<<.............>>..
..<<<<<<<<<.<<<....<<<<....<<<<<......>>>>>...>>>>
...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c3a New crystal system to promote screening for new eukaryotic inhibitors
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): V2 R3 Y4 S16 Y21 R23 S25 F26 K27 N28 R30 Q34 K43 Q50 R56 R62 Y63 S65 S66 I67 G68 R69 G71 Q72 K74 E75 K80 R82 W83 P84 A85 K86 R96 N97 A100 N101 Q118 V119 H121 R127 T129 Y130 R131 A132 H133 G134 R135 I136 N137 A138 Y139 Q140 S141 T142 R167 N169 S170 R171 Q172 G174 R175 S178 L182
• Binding residue (residue number reindexed from 1): V1 R2 Y3 S15 Y20 R22 S24 F25 K26 N27 R29 Q33 K42 Q49 R55 R61 Y62 S64 S65 I66 G67 R68 G70 Q71 K73 E74 K79 R81 W82 P83 A84 K85 R95 N96 A99 N100 Q117 V118 H120 R126 T128 Y129 R130 A131 H132 G133 R134 I135 N136 A137 Y138 Q139 S140 T141 R158 N160 S161 R162 Q163 G165 R166 S169 L173

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8c3a, PDBe:8c3a, PDBj:8c3a
• PDBsum: 8c3a
• UniProt: A0A8H6BUG0