Structure of PDB 7zs5 Chain BK Binding Site BS01

Receptor Information

>7zs5 Chain BK (length=220) Species: 4932 (Saccharomyces cerevisiae)

ARRPARCYRYQKNKPYPKSRYNRAVPDSKIRIYDLGKKKATVDEFPLCVH
LVSNELEQLSSEALEAARICANKYMTTVSGRDAFHLRVRVHPFHVLRINK
MLSCAGADRLQQGMRGAWGKPHGLAARVDIGQIIFSVRTKDSNKDVVVEG
LRRARYKFPGQQKIILSKKWGFTNLDRPEYLKKREAGEVKDDGAFVKFLS
KKGSLENNIREFPEYFAAQA

Ligand information

>7zs5 Chain 1 (length=3248)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
aucucgcauuucacugggccagcaucaguuuugguggcaggauaaaucca
uaggaauguagcuugccucgguaaguauuauagccugugggaauacugcc
agcugggacugaggacugcgacguaagucaaggaugcuggcauaaugguu
auaugccgcccgucuugaaacacggaccaaggagucuaacgucuaugcga
guguuuggguguaaaacccauacgcguaaugaaagugaacguagguuggg
gccucgcaagaggugcacaaucgaccgauccugaugucuucggauggauu
ugaguaagagcauagcuguugggacccgaaagauggugaacuaugccuga
auagggugaagccagaggaaacucugguggaggcucguagcgguucugac
gugcaaaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaac
caucuaguagcugguuccugccgaaguuucccucaggauagcagaagcuc
guaucaguuuuaugagguaaagcgaaugauuagagguuccggggucgaaa
ugaccuugaccuauucucaaacuuuaaauauguaagaaguccuuguuacu
uaauugaacguggacauuugaaugaagagcuuuuagugggccauuuuugg
uaagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccg
gaauacacgcucaucagacaccacaaaagguguuaguucaucuagacagc
cggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuc
accggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuac
cuauacucuaccgucaggguugauaugaugcccugacgaguaggcaggcg
uggaggucagugacgaagccuagaccguaaggucgggucgaacggccucu
agugcagaucuuggugguaguagcaaauauucaaaugagaacuuugaaga
cugaaguggggaaagguuccacgucaacagcaguuggacguggguuaguc
gauccuaagagauggggaagcuccguuucaaaggccugauuuuaugcagg
ccaccaucgaaagggaauccgguuaagauuccggaaccuggauauggauu
cuucacgguaacguaacugaauguggagacgucggcgcgagcccugggag
gaguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccg
gagauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggu
gcgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcc
aggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguu
gauagaauaauguagauaagggaagucggcaaaauagauccguaacuucg
ggauaaggauuggcucuaagggucggguagugagggccuuggucagacgc
uuguuguagacggccuuggucuugcuacaauuaacgaucaacuuagaacu
gguacggacaaggggaaucugacugucuaauuaaaacauagcauugcgau
ggucagaaagugauguugacgcaaugugauuucugcccagugcucugaau
gucaaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgca
ugaauggauuaacgagauucccacugucccuaucuacuaucuagcgaaac
cacagccaagggaacgggcuuggcagaaucagcggggaaagaagacccug
uugagcuugacucuaguuugacauugugaagagacauagagggcuaccuu
uauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuuccac
guucuagcauucaaggucccauucggggcugauccggguugaagacauug
ucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacgcag
auguccuaaggggggcucauggagaacagaaaucuccaguagaacaaaag
gguaaaagcccccuugauuuugauuuucagugugaauacaaaccaugaaa
guguggccuaucgauccuuuagucccucggaauuugaggcuagaggugcc
agaaaaguuaccacagggauaacuggcuuguggcagucaagcguucauag
cgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccgaag
cagaauucgguaagcguuggauuguucacccacuaauagggaacgugagc
uggguuuagaccgucgugagacagguuaguuuuacccuacugaugaaugu
uaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggaua
auugguuuuugcggcugucugaucaggcauugccgcgaagcuaccauccg
cuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcggu
gauuucuuugcuccacacaauauagauggauacgaauaaggcguccuugu
ggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggc
cuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaauc
auuuguauacgacuuagauguacaacgggguauuguaagcaguagaguag
ccuuguuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<<....>>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.((......<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<....<<..
......>>....>>>..<<<<<<<<<<<<<<<<<<<<<<<.....<<<<<
<<<<......<<<<<<....>>>>>>.......>>>>>>>>..>.>>>>>
.>>>>>>>>>>........<<<....>>>....>>>>>>>>.....>>>>
>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..
<<<...<<<<.....>>>>...>>>...<<....>>.......<<<<<..
<<<<<....>>>>>...>>>>>.<<<<<<<<...<......>...>>>.>
>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<
<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.
.......>>>>.>>>....>>>>>>>>>...<<.......>>......>>
>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<
...<<<.<<<<<<<.....<<<....<<<....<<<<<..<<<<<<....
.>>>>>>>>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<.
.....>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.
....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.
....<<<<<<.....<<..........<<<.<<<<<<<<<........<<
<<<.<<<...<<.........>>...>>>.........<<......>>..
.>>>>>....>>>>>>>>>>>>..........))).>>...>>>>>>.>>
>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>>..
<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>
>........>>>>>><<.....<<.....<<<<<<.......>>>>>><<
<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<<<...<<....<<<....>>>........<<.<<<........>.>>
>>....>>....>>>..<<<<<........>>>>>))<<<<.<<<<<<<.
....<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<<<<<<<<<
<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>
....>>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.
>.>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>>>
>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>
..<<<<.....<<<<<<<.<<<........<.....<<<<<....<<..>
>.....>>>>><<.<<<<<<<<<<<.<<<<<<........<<<.....<.
......>.....>>>.......>>>>>>......>>>>>..>>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.....
<<.....<<<<....>>>>.....>>..>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<.......>>>>>.....
>.....>>....>>.>>>>>>>..>.>>>.>>>>>>>..>>>>>>....>
>....<<<<<........>>>>>....<<<<<..<<<.<<......<<<<
.<<<<<<..<<<<<.<<<<<<<<..<<.<<<<<<<<<<<<<......>>>
>>>.>>>>>>>.>>...<<<<<.....<<<<..<<<<<......>>>>>.
.........}}...<<<<<<....>>>>>>..>>>>..>>>>>.....>>
>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....
>>>.>>)))......<<<<<<<..<<<<.))))))>>>>>>>>>>>....
.....>>>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>
>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<.
.....>>>>>>>...>.<<<.........>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<
<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<.
..<<<<<...<<<<<<<<<<]]].>>>>>..>>>>>..>>>>>..>>>>>
.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>
>>>.......<<<<<<.<<<.<<....<<..........<<<<<<.....
>>>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>
>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<
.<.............>.....<<<<<<<<<.<<<....<<<<.<..<<<<
<......>>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zs5 Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): R2 R3 A5 R6 C7 R9 K12 K14 P15 Y21 R23 K39 E62 A66 R89 H91 F93 R97 L110 Q112 M114 R115 G116 A117 W118 R153 Y156 K157 P159 D192 G193 A194 F195 K197
• Binding residue (residue number reindexed from 1): R2 R3 A5 R6 C7 R9 K12 K14 P15 Y21 R23 K39 E62 A66 R89 H91 F93 R97 L110 Q112 M114 R115 G116 A117 W118 R153 Y156 K157 P159 D192 G193 A194 F195 K197

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zs5, PDBe:7zs5, PDBj:7zs5
• PDBsum: 7zs5
• PubMed: 36801861
• UniProt: P41805|RL10_YEAST Large ribosomal subunit protein uL16 (Gene Name=RPL10)