Structure of PDB 4v65 Chain BJ Binding Site BS01

Receptor Information

>4v65 Chain BJ (length=117) Species: 562 (Escherichia coli)

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRR
QRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVF
DKVAFTALVEKAKAALA

Ligand information

>4v65 Chain BB (length=2903)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaagggggucaucccgacuuaccaa
cccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacgg
cgggugcuaacguccgucgugaagagggaaacaacccagaccgccagcua
aggucccaaagucaugguuaagugggaaacgaugugggaaggcccagaca
gccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagc
ucacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugc
accgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguuc
uguaagccugcgaaggugugcugugaggcaugcuggagguaucagaagug
cgaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgg
aagaccaaggguuccuguccaacguuaaucggggcagggugagucgaccc
cuaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccu
guacuugguguuacugcgaaggggggacggagaaggcuauguuggccggg
cgacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccg
gaaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaac
aaaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaauc
guaccccaaaccgacacagguggucagguagagaauaccaaggcgcuuga
gagaacucgggugaaggaacuaggcaaaauggugccguaacuucgggaga
aggcacgcugauauguaggugaggucccucgcggauggagcugaaaucag
ucgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugc
aaacacgaaaguggacguauacggugugacgccugcccggugccggaagg
uuaauugaugggguuagcgcaagcgaagcucuugaucgaagccccgguaa
acggcggccguaacuauaacgguccuaagguagcgaaauuccuugucggg
uaaguuccgaccugcacgaauggcguaaugauggccaggcugucuccacc
cgagacucagugaaauugaacucgcugugaagaugcaguguacccgcggc
aagacggaaagaccccgugaaccuuuacuauagcuugacacugaacauug
agccuugauguguaggauaggugggaggcuuugaaguguggacgccaguc
ugcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaac
guugacccguaauccggguugcggacagugucugguggguaguuugacug
gggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaauc
cuggucggacaucaggagguuagugcaauggcauaagccagcuugacugc
gagcgugacggcgcgagcaggugcgaaagcaggucauagugauccggugg
uucugaauggaagggccaucgcucaacggauaaaagguacuccggggaua
acaggcugauaccgcccaagaguucauaucgacggcgguguuuggcaccu
cgaugucggcucaucacauccuggggcugaaguaggucccaaggguaugg
cuguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugaga
caguucggucccuaucugccgugggcgcuggagaacugaggggggcugcu
ccuaguacgagaggaccggaguggacgcaucacugguguucggguuguca
ugccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaaa
gcaucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaa
ggguccugaaggaacguugaagacgacgacguugauaggccgggugugua
agcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaa
ccu

<<<<<<<<.......<<.<<<<<<..........<<<<<<<<<<<<<...
...<<<...................<<<<<....<<<.......>>>...
.....>>>>>...>>>....<<......>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<.<............
........>>>>..>........<<....>>...<<<<<...........
.......>>>>>...<<<..<<<<......<<<<<<<<<<<<<.<.....
.<<<........>>>.........<<<.......>>>.....>>>...>>
>>>>>>>>>...>>>>........<...<<.............>>...>.
......<<<<..(((.>.>>>..>>>......>>.>>>>>>>.>>.....
.................................<<<<.....>>>>....
...............>>>>>>.>>.........<<....<<<<......>
>>>....>>...................<<........<<.....<<<.<
<........<<.....>>.........<<....>>..<<<.......>>>
.....>>.>>>.....>>..<<.....<<<...............<...<
<.<...<<<<<<......>>>>>>.>....>>....<.............
........>.......>.........<<.......>>.........>>>.
.......>>..<<<<<.....<..<<....>>..>..<<<<<<<...<<<
<<<<<.<....<<<...<<..<<<<...<<<<<<<.....>>>>>.>>..
.>>>>..>>..>>.>.....>>>>>>>.>>...>>>>>>>..........
<..<........>..>........................<<<<.<<...
.....(......<<<<<<...........<<..<<<<<..<<......<.
<<<<<.<<<..<<<.........>>>..>>>........<<<......>>
>..>>>>>.>..>>..>>>>>.>>............)......>>>>>>.
.......>>>>>><<<<<<<<<....>>>>>>>>>.....>>>>>....<
<<....<..........<<.<<....>>.>>.......>...>>>.....
.........>>...............<<<<<.......>>>>>.<<<<..
..........<..<<<<<...............>>>>.>.....<<..<.
<...<<<...<<<....>>>...>>>...>.>..<<<<..........>>
>>..<<<<<<<<<<<<<...<<<<....<<<...<<<..<<<<<.<<<<<
....[[....>>>>>.....<<<....<<<<<<<<<<<<.<<.....>>>
>>>>>>>..>>>>....>>>........<<<<<....>>>>>....>>>>
>...>>>....>.>>........>>>>...>>....>>>>>>>>>>>..>
>..>......................<<.........>>..>>>>...<<
.........<<......................<<...............
..>>..<<<<<......<.<...<<<<.....>>>>...>.>....>>>>
>............<<<.<<.........................<<<...
.....................>>>.....<<<<........<<<<<..<<
<........<<<<<<.<<....>>..>>>>>>........>>>>>>>>..
......<<<<<.......>>>>.>......................<...
........>............>>>>.......>>.>>>..........>>
......>>...............<..............>...<<<<....
......<...............<<....<...<<<.<<<<..<<<<<<<<
<<<.<<<............<<<<<..<<.......<<<<<<<......>>
>>>>>....>>............>>>>>...>>>.>>>>>>>>>>>...<
<<.<<<<<.......>>>>>>>>.....>>>>.>>>....>.....<<..
.....>>..<<<<<...........>>>>>........<<<<<......<
<<<.........>>>>............((........>>>>>...<<<<
..<<<<....>>>>..>>>>..............)).........<<<<<
<.<<...)))..>>>>>>>>..............>>..............
....<......<<<<<...<<.......>>...>>>>>.......>....
...................<<<..<<.<......>..>>...>>>..<<.
........>>...........................<<..<<......>
>...>>....>..........>>>>....<<<<<<<...<<<<...<<<<
<<...............>>>>>.>..<<<<....<<<.....<<<..<<<
..]]..>>>....>>>.....>>>..>>>>.....<<....<<<<<....
.......>>>>>....>>..>>>>.......>>>>>>>..<<<<<<...>
>>>>>.....<<...<<<.........>>>.....<..<<<<<...<...
.<<<<<....>>>>>...>....>>>>>...>.....>>.....>>>>>>
>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v65 The Structure of the E. coli Ribosome Before and After Accommodation: Implications for Proofreading
• Resolution: 9.0 Å
• Binding residue (original residue number in PDB): A1 R2 K4 R5 G6 V7 I8 R12 K15 I16 K18 A20 K21 Y23 Y24 R27 R29 V30 Y31 R32 F35 Q36 K40 Y44 Y46 R47 R49 R50 Q51 R52 K53 R54 Q55 R57 Q58 R69 S74 Y75 S76 K77 I79 N80 K83 R91 K92
• Binding residue (residue number reindexed from 1): A1 R2 K4 R5 G6 V7 I8 R12 K15 I16 K18 A20 K21 Y23 Y24 R27 R29 V30 Y31 R32 F35 Q36 K40 Y44 Y46 R47 R49 R50 Q51 R52 K53 R54 Q55 R57 Q58 R69 S74 Y75 S76 K77 I79 N80 K83 R91 K92

Gene Ontology

Molecular Function

• GO:0000900 mRNA regulatory element binding translation repressor activity
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v65, PDBe:4v65, PDBj:4v65
• PDBsum: 4v65
• UniProt: P0A7L3|RL20_ECOLI Large ribosomal subunit protein bL20 (Gene Name=rplT)