Structure of PDB 4v6a Chain BF Binding Site BS01

Receptor Information

>4v6a Chain BF (length=207) Species: 300852 (Thermus thermophilus HB8)

MKEVAVYQIPVLSPSGRRELAADLPAEINPHLLWEVVRWQLAKRRRGTAS
TKTRGEVAYSGRKIWPQKHTGRARHGDIGAPIFVGGGVVFGPKPRDYSYT
LPKKVRKKGLAMAVADRAREGKLLLVEAFAGVNGKTKEFLAWAKEAGLDG
SESVLLVTGNELVRRAARNLPWVVTLAPEGLNVYDIVRTERLVMDLDAWE
VFQNRIG

Ligand information

>4v6a Chain BA (length=2767)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
uuuugcgcggggggaaccuggggaacugaaacaucucaguacccagagga
gaggaaagagaaaucgacucccugaguagcggcgagcgaaaggggaccag
ccuaaaccguccggcuuguccgggcggggucguggggcccucggacaccg
aauccccagccuagccgaagcuguugggaagcagcgccagagagggugaa
agccccguaggcgaaagguggggggauaggugaggguacccgaguacccc
gugguucguggagccaugggggaaucugggcggaccaccgccuaaggcua
aguacuccgggugaccgauagcgcaccaguaccgugagggaaaggugaaa
agaaccccgggaggggagugaaauagagccugaaaccgugggcuuacaag
cagucacggccccgggguuguggcgugccuauugaagcaugagccggcga
cucacggucgugggcgagcuuaagccguugaggcggaggcguagggaaac
cgaguccgaacagggcgcaguccgcggccguggacccgaaaccgggcgag
cuagcccuggccagggugaagcuggggugagacccaguggaggcccgaac
cggugggggaugcaaaccccucggaugagcuggggcuaggagugaaaagc
uaaccgagcccggagauagcugguucuccccgaaaugacuuuagggucag
ccucaggcgcugacuggggccuguagagcacugauagggcuagggggccc
accagccuaccaaacccugucaaacuccgaagggucccagguggagccug
ggagugagggcgcgagcgauaacguccgcguccgagcgcgggaacaaccg
agaccgccagcuaaggcccccaagucugggcuaagugguaaaggaugugg
cgccgcgaggucgaguggcgccgcgccgaaaaugaucggggcuuaagccc
agcgccgaagcugcgggucugggggaugaccccaggcgguaggggagcgu
ucccgaugccgaugaaggccgacccgcgagggcggcuggagguaagggaa
gugcgaaugccggcaugaguaacgauaaagagggugagaaucccucucgc
cguaagcccaaggguuccuacgcaauggucgucagcguaggguuaggcgg
gaccuaaggugaagccgaaaggcguagccgaagggcagccgguuaauauu
ccggcccuucccgcaggugcgauggggggacgcucuaggcuagggggacc
ggagccauggacgagcccggccagaagcgcagggugggagguaggcaaau
ccgccucccaacaagcucugcgugguggggaagcccaaccccccgaagcc
agggagccaagaaaagccucuaagcacaaccugcgggaacccguaccgca
aaccgacacaggugggcgggugcaagagcacucaggcgcgcgggagaacc
cucgccaaggaacucugcaaguuggccccguaacuucgggagaaggggug
cucccuggggugaugagccccggggagccgcagugaacaggcucuggcga
cuguuuaccaaaaacacagcucucugcgaacucguaagaggagguauagg
gagcgacgcuugcccggugccggaaggucaaggggaggggugcaagcccc
gaaccgaagccccggugaacggcggccguaacuauaacgguccuaaggua
gcgaaauuccuugucggguaaguuccgaccugcacgaaaagcguaacgac
cggagcgcugucucggcgagggacccggugaaauugaacuggccgugaag
augcggccuacccguggcaggacgaaaagaccccguggagcuuuacugca
gccugguguuggcucuuggucgcgccugcguaggauaggugggagcaggc
gccggugaaauaccacccuggcgcggcugggggccuaacccucggauggg
gggacagcgcuuggcgggcaguuugacuggggcggucgccuccuaaaagg
uaacggaggcgcccaaagguccccucaggcgggacggaaauccgccggag
agcgcaaggguagaagggggccugacugcgaggccugcaagccgagcagg
ggcgaaagccgggccuagugaaccgguggucccguguggaagggccaucg
aucaacggauaaaaguuaccccggggauaacaggcugaucucccccgagc
guccacagcggcggggagguuuggcaccucgaugucggcucgucgcaucc
uggggcugaagaaggucccaaggguugggcuguucgcccauuaaagcggc
acgcgagcuggguucagaacgucgugagacaguucggucucuauccgcca
cgggcgcaggaggcuugaggggggcucuuccuaguacgagaggaccggaa
gggacgcaccucugguuucccagcugucccuccaggggcauaagcugggu
agccaugugcggaagggauaaccgcugaaagcaucuaagcgggaagcccg
ccccaagaugaggccucccacggcgucaagccgguaaggacccgggaaga
ccacccgguggaugggccggggguguaagcgccgcgaggcguugagccga
ccggucccaaucguccg

.........<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<<
....>>>>>>.>>...<<<<<<.....<<....>>......>>>>>.>..
.......<<....>>...<<<<<.....<<.....>>....>>>>>...<
<<....<<<<<<<<.....>>>>>>>>......<<<<<<<<.......<.
..<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<....
.))>>>...>>>...>>>>..>>>>>..>...>>>>>.>>>.....<<<<
<<<<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>..
....>>.>>>>>>.>>...............<<....>>....>>.....
....<<<<....>>>>..................>>>>>>>>>>>.....
..<<<<<<<<<<..>>>>>>>>>>...<............>.<<<<<<..
..<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<<....>
>..<<<<.....>>>>...>>>>>>>>>>>>>..<<.<...<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<..<<<<........>>>>.>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<.....<<<<<<<<<...<<<...<<<<<<<<.<.<<<<<<..
....>>>>.>>>..>>>>>>>>..>>.>...>>>>>>>>>.....>>>>>
>>.>>..<<<<<<<.<<......>>.>>>>>>>.....<<<......>>>
...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<<<
<<<<<..........>>>>>>>>>.>>..........)))......>>>>
>>.>>>...>>>>>>><<<<<<<.......>>>>>>>....>>>>>>..<
<<<<...<<<......<<<<<.<<<....>>>>>>>>...>>>..>>>>>
........>>>>>><......<<.....<<<<<<.......>>>>>><<<
<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<
<.<<...<<<...<<<....>>>...>>>...>>...<<<<<........
>>>>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..<<<<<.<<
<<.<<..[[[[...>>>>>><<<...<<<<<<<<<<<<<<<<.<<.....
>>>>>>>>>.....>>>>>>>>>..>>><.........>>>>>>...>>>
..>>>>>.........>>>>...>>...>>>>>>>>>>>>>>>..>>...
..<<......>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<
<<<<<<.<<.........<.....<<<<<...............>>>>><
<<<<<<<<<<<.....>>>>>>>>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>
...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>........
<...........<<<<<<.....>>>>>>.....>....>>>>>..>>.>
>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<.....
...>>>>>..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.
<<<.<<<<<.<<<<<<<<<<<<<<<<...........<<<<<..<....>
............>>>>>..>>>>>>>>>>>>>>>>...<<<<<....>>>
>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<...(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.
<<<....>>>.>>))).......<<<<<<<.<<..))))..>>>>>>>>>
.............>>>>..>>>>>>.......<<<<...<<<<<<...<<
.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<
<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>........>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<<...............>>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>..
.>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.
>>>>.......>>>>>>>...<.........>.....<<<<<<<<<....
...>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..
>>>>>.>>>...>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v6a Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): H31 L32 W34 R38 Q40 L41 A42 K43 R44 R45 R46 T48 A49 S50 T51 T53 R54 G55 V57 A58 Y59 S60 G61 R62 K63 Q67 K68 H69 G71 R72 A73 R74 H75 G76 P81 I82 F83 V84 V88 V89 F90 P92 R95 Y99 T100 L101 P102 K103 R106 K108 K135 T136 R165 R168 N169 P171 G180 N182 Y184 R205
• Binding residue (residue number reindexed from 1): H31 L32 W34 R38 Q40 L41 A42 K43 R44 R45 R46 T48 A49 S50 T51 T53 R54 G55 V57 A58 Y59 S60 G61 R62 K63 Q67 K68 H69 G71 R72 A73 R74 H75 G76 P81 I82 F83 V84 V88 V89 F90 P92 R95 Y99 T100 L101 P102 K103 R106 K108 K135 T136 R165 R168 N169 P171 G180 N182 Y184 R205

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v6a, PDBe:4v6a, PDBj:4v6a
• PDBsum: 4v6a
• PubMed: 19696344
• UniProt: Q5SHN9|RL4_THET8 Large ribosomal subunit protein uL4 (Gene Name=rplD)