Structure of PDB 8cq7 Chain BE Binding Site BS01

Receptor Information

>8cq7 Chain BE (length=208) Species: 5476 (Candida albicans)

ARRPARCYRYCKNKPYPKSRYNRAVPDAKIRIYDLGRKKATVDEFPLCIH
LVSNELEQLSSEALEAARICANKYITKVSGRDSFHLRVRVHPFHVLRINK
MQGMRGAWGKPHGLAARVSIGQIIMSARTKDSNKDVVIEGLRRARYKFPG
QQKIIISKKWGFTPLNRDEYIAKKTNGEVIDDGAYVKFLSRKGNLEANLQ
QFPNYQYS

Ligand information

>8cq7 Chain AS (length=3222)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuugca
ugccgcugcgguuuaccgggccagcaucgguuuggagcggcaggauaaug
gcggaggaauguggcacggcuucugcuguguguuauagccucugacgaua
cugccagccuagaccgaggacugcgguuuuuaccuaggauguuggcauaa
ugaucuuaagucgcccgucuugaaacacggaccaaggagucuaacgucua
ugcgaguguuuggguguaaaacccguacgcguaaugaaagugaacgaagg
ugggggcccauuagggugcaccaucgaccgauccugauguguucggaugg
auuugaguaagagcauagcuguugggacccgaaagauggugaacuaugcc
ugaauagggugaagccagaggaaacucugguggaggcucguagcgguucu
gacgugcaaaucgaucgucgaauuuggguauaggggcgaaagacuaaucg
aaccaucuaguagcugguuccugccgaaguuucccucaggauagcagaag
cucguaucaguuuuaugagguaaagcgaaugauuagaagucuugggguug
aaaugaccuuaacuuauucucaaacuuuaaauauguaagaaguccuuguu
gcuuaauugaacguggacaauugaaugaagagcuuuuagugggccauuuu
ugguaagcagaacuggcgaugcgggaugaaccgaacgugaaguuaaagug
ccggaaugcacgcucaucagacaccacaaaagguguuaguucaucuagac
agccggacgguggccauggaagucggaauccgcuaaggaguguguaacaa
cucaccggccgaaugaacuagcccugaaaauggauggcgcucaagcgugc
uacuuauacuucaccgugauugcuguuuugacgcuuucacgaguaggcag
gcguggaggucagugacgaagccuuugcuguaaagcugggucgaacggcc
ucuagugcagaucuuggugguaguagcaaauauucaaaugagaacuuuga
agacugaaguggggaaagguuccaugucaacagcaguuggacauggguua
gucgauccuaagagauggggaagcuccguuucaacgugcuugauuuuuca
ggccaaccaucgaaagggaauccgguuaaaauuccggaacuuggauaugg
auucuucacggcaacguaacugaauguggagacgucggcgugagcccugg
gaggaguuaucuuuucuucuuaacagcuuaucacccuggaauugguuuau
ccggagauggggucuuauggcuggaagagcgcgguaauuuugccgcgucc
ggugcgcuuacgacgguccuugaaaauccacaggaaggaauaguuuucau
gccaagucguacucauaaccgcagcaggucuccaagguuaacagccucua
guugauagaauaauguagauaagggaagucggcaaaauagauccguaacu
ucgggauaaggauuggcucuaaggaucgguaacgaucaacuuagaacugg
uacggacaaggggaaucugacugucuaauuaaaacauagcauugugaugg
ucagaaagugauguugacacaaugugauuucugcccagugcucugaaugu
caaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacuaug
acucucuuaagguagccaaaugccucgucaucuaauuagugacgcgcaug
aauggauuaacgagauucccacugucccuaucuacuaucuagcgaaacca
cagccaagggaacgggcuuggcagaaucagcggggaaagaagacccuguu
gagcuugacucuaguuugacauugugaaaagacauggaggguguagaaua
agugggagcuucggcgccggugaaauaccacuaccucuauaguuuuuuua
cuuauucaaugaagcggagcuggaggucaaacuccacguucuagcauuaa
gcccucugggcgauccggguugaagacauugucagguggggaguuuggcu
ggggcggcacaucuguuaaacgauaacgcagguguccuaagggggacuca
uggagaacagaaaucuccaguagaacaaaaggguaaaaguccccuugauu
uugauuuucagugugaauacaaaccaugaaaguguggccuaucgauccuu
uagucccucggaauuugaggcuagaggugccagaaaaguuaccacaggga
uaacuggcuuguggcagucaagcguucauagcgacauugcuuuuugauuc
uucgaugucggcucuuccuaucauaccgaagcagaauucgguaagcguug
gauuguucacccacuaauagggaacgugagcuggguuuagaccgucguga
gacagguuaguuuuacccuacugaugaauguuaucgcaauaguaauugaa
cuuaguacgagaggaaccguucauucagauaauugguuuuugcggcuguc
ugaucaggcaacgccgcgaagcuaccaucugcuggauuauggcugaacgc
cucuaagucagaauccaugcuagaacgcgaugauuuuugcccugcacauu
uuagauggauacgaauaagacuuuuuagucgcuggaccauagcaggcugg
caacggugcgcuuagcggaaaggcuuugugcgcuugccggcggauagcaa
ugucaacaugcgcggggauaaauccuuugcauacgacuuagauguacaac
ggaguauuguaagcaguagaguagccuuguuguuacgaucugcugagauu
aagcucuuguugucugauuugu

.......................<<<<<<.....<......>......>>
>>>.>.........<<....>>..<<<<<......<<.....>>.....>
>>>>..<<<...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<.....<<<<......>>>
>.(.......<<<......)>>>....>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<...<<<<
......>>>>...>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.
<<<<<<<......<<<<<<......>>>>>>.......>>>>>>.>.>>.
>>>>>.>>>>>>>>>>........<<<....>>>.....>>>>>>>....
>>>>>>>>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<
<<<..<<<..<<<<<.....>>>>>..>>>...<<....>>.......<<
<<<..<<<<....>>>>...>>>>>.<<<<<<<<...<<....>>...>>
>.>>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<
<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.
<<........>>>>.>>>....>>>>>>>>>...<<.......>>.....
.>>>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<
<<<...<<<.<<<<<<<.....<<<....<<<....<<<<...<<<<<..
.....>>>>>.>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<
.<......>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<
<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(
((.....<<<<<<.....<<..........<<<.<<<<<<<<<<......
.<<<<<.<<<...<<.........>>...>>>.........<<......>
>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>
.>>>>..>>>>>>><<<<<..<<.........>>..>>>>>....>>>>>
>..<<<<<<<<.<<......<<<<..<<<....>>>.>>>>...>>>>>>
>>>>........>>>>>><<.....<<.....<<<<<<.......>>>>>
><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<<<...<<....<<<....>>>..........<<<<<<....>>>
>>>......>>....>>...<<<<<........>>>>>))<<<<.<<<<<
<<.....<<<....>>>......{<<<<<<...<<<<.<<<<<<<.<<<<
<<<<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....
>>>....>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>
>>...>>>>>>>.>>>>.........>>>>>>..........>>>..>>>
>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>
>>>..<<<<.....<<<<<<<.<<.........<.....<<<<<......
.........>>>>><<.<<<<<<<<<<<....>>>>>..>>>>>>.>>..
..........<<<<<<<<<..<<<.........>>>..<<<<<<<....<
<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<
.....<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......
>>>>>>........<...........<<<<<.......>>>>>.....>.
....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>.
...<<<<<........>>>>>....<<<<<..<<<.<<......<<<<.<
<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<<<<...<<..........
.......<......>....................>>.>>.>>>>>>>>>
>...<<<<<.....<<<<..<<<<<......>>>>>..........}...
<<<<...>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<
.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...
<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<
<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))......<<<<
<<<..<<<<.))))))>>>>>>>>>>>.........>>>>..>>>>>>..
.....<<.<...<<<<<<...<<.......>>...>>>>>>....>.>.>
.......<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<
<.........>>>.....>>>>>..>...>>>.>>....<<<<<<<....
>>>.>>>>....>>..>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<
<.................>>>>>.><<<<<...<<<<....<<<<<<<<<
<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<......
.....>>>>>...>>>>.>>>>....>>>>>>>>.....<<<<<<.<<<.
......<<..........<<<<....>>>>......>>.......<<<<<
<...<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>.........
.......>>>.>>>>>>..<<<<<<<.............>>....<<<<<
<<<<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>.
...>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cq7 Drug-induced rotational movement of the ribosome is a key factor for read-through enhancement
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): R3 R4 A6 R7 C8 K13 N14 K15 Y17 Y22 R24 Y34 D35 K39 S61 E63 A64 A67 K74 R90 H92 F94 R98 N100 G114 M115 R116 W119 G120 Q133 R154 Y157 K158 P160 D193 G194 A195 Y196 K198
• Binding residue (residue number reindexed from 1): R2 R3 A5 R6 C7 K12 N13 K14 Y16 Y21 R23 Y33 D34 K38 S60 E62 A63 A66 K73 R89 H91 F93 R97 N99 G103 M104 R105 W108 G109 Q122 R143 Y146 K147 P149 D182 G183 A184 Y185 K187

External links

• PDB: RCSB:8cq7, PDBe:8cq7, PDBj:8cq7
• PDBsum: 8cq7
• UniProt: Q5AIB8|RL10_CANAL Large ribosomal subunit protein uL16 (Gene Name=RPL10)