Structure of PDB 7pwo Chain B2 Binding Site BS01

Receptor Information

>7pwo Chain B2 (length=378) Species: 184922 (Giardia lamblia ATCC 50803)

SHRKFSCCRKGNLGYLPRKRCTRGRGRCKTFPPDVQGAPIHLTAFLGYKA
GCTHVMRFIDHRGSNLHNRQAIDQATIIDAPPMICTAIIGYAKTPKGLRA
VTTVWAAHIAEPAMRRFYRNYFHAEKTAFSTYMKKAADGTYIKEQLGRLK
QHADVIRIVAHTQPALTPLKQKKADIMEIQVNGGANVAEKVDYAYALMEK
PISIKDVFSVGAQIDTISITRGRGFEGVVTRWGVTRLPRKTRRGNRKVAC
IGAWHPANVQYTVARAGQMGYFHRTDTNKQVFMIDTAENPRCCTTEFDLT
DKSINPVGGFVNYGRVQGDFIMIKGTCPGPKRRPVVMRNALLPHRNCPPV
QLQWISTASKFGHGIFETVEERRQFYAK

Ligand information

>7pwo Chain 1 (length=2448)

cggcccgaggcggcgggggcgacgggcggaacuuaagcauaucaguacgc
cccggaggagaaaccaaccgggauuccccguagcggcgagcgacgcggga
ggagcccgccccgaaggcgcgcugugggcgccgcgagggggcccgagggc
cccgcccgagagggugcaagccccguacggcgggcgaguagcgcugcuug
agcgugcagcgcgaagggaggcgcggcccuuccaaggcuaaauacgcccc
gggaccgauagcggaccaaguagcgcgagcgaacggugaaaaggacgccc
ggccgcucaaaagaccugaacccggccggcgccggcccgccggccccguc
ucgaaacacggaccgaggagccacgcgccgcggcgagcccgagggccccg
cggcggagcgagcgcgagacgccccgggcccgccgcgccccugcgggcgu
gcgcgggccgagccgcggcgcgugggcccgaaaggcggugaucuaugccc
ggcgagggcgaggccgggcgaaagccugguggaggcccgccgcggugcug
acgcgcagaucgcucgucggagccgggcaugggggcgaaagacucaucga
accgccugguagcugguugccuccgaaaugucucccaggacagccgccgc
ccgcaguugcggcccguagagcgcuggccggcggccgccccccaaacucc
gaagggccgcgccgccccgcccuggccugggcggggcgggcgaaugcggg
cggcgcgugggccccuccugguaagcaggacgggcgaggcgggacgaucc
ggacgccgggccagggugcgccgccggggccggaacggcgucggccgcgg
ccggcccggaaaauggagcgcgccggagccccggacccgcgcccggccgc
cgcgcgcggcggguaggaggccgcagaggccccgggggcgaaggcggcgc
gcaggccccgccggaccggccucuggugcagaucucggcagcaguagcgc
uacuccgcccccggaggacugagggggagacggguuccgcggcgccugca
ucuggccgcgggugacucgggccuaagcggcgggugaagaccgggaaggg
gcgugcccgcccgucgaacggggagccggcggagacuccggcaggcgcgg
gcggagacgcgccccccggcgacgcgcacggggaccgcggcgggcggcgc
cccggcccgcgaacgccccgcagcccccggacgccuugcgcggagagggg
ggcccgggggcggaccgcgcguccccggccgccccugaaaagccgggggg
cccgcgcgccguaccgaccgcacaggacuccggggucagcagccucuagc
gcgggagcgaacgcggcucagggaagucggcaagccggcuccguaaccuc
gggaaaaggaguggcucugacggggucagaacuggaacggacgcggggau
cccgacuguuuacuagaaacacagcgucgcgagggccgcacccggcgcug
gcgcgacgugauuucugcccagugccagaccgucaccgugaagcgauccg
ccgaagcccugguaaacggcgggaguaaugacucucuuaagguagccaaa
ugccucgucgggcaauuuccgacggcaugaauggaccaacgaggauccca
cugucccgagccgcgccuccgcgagccuccagccucgggaacgggcgagg
gccggccagcggggcaagaagacccuuuugagcuugacuccagcccgggc
cuguggggcggggccgccccgcucacccggucgcgcggggacccgcccgg
gcggggaguucggcuggggcggcgcgccugcuacaccggaccgcaggcgu
cccacggcgggcucagcgaggacccucccgcggagcagaagggcacaagc
ccgcccgacccgcgccccccgugccggcgcgggccgcgaaagcggggccu
accgauccuucgccgccccggccgcgggcgcggagguggcagaaaaguua
ccacagggauaacuggcuuguggccgccgagcgcccgcagcgacgcggcu
uuuugauccuucgaugucggcucuuccuaccguccgcgcgcaccggcgcg
gaagcgucggauuguucacccguucaagggaucgugagcuggguuuagac
cgucgugagacgguuaguuuuacccuacuggccccggggccagagcacgg
cgggccaguacggaggaacgcccgccgcgggcgcccagccccgcgguugc
ccgccggggcagaccgcgcgcccgggcccgggggccuggcgcugccgccu
cuaaagcgccaccccccccuccggccccgccgcccgcgccccagccccgu
gcccccugcccgaggcggcccccgcccgggaggaccacccggcgcggcgc
cccuguacggcgagggcgcguucgcccggggggcgcgccgcaggcgcg

......................<<<<<<.....<......>......>>>
>>.>.........<<....>>..<<<<<....<<.....>>.....>>>>
>..[[[...<<<<...........>>>><<<<..<..<<<<<......>>
>>>((......<<<.....))>>>...>>>>>........<<<<<<<...
.....>>>>>>>...(((((..<<<<<<)))))..]]]............
......................<<....>>................<<<.
>>>...........................<<<<<<..>>>>>>.<<<<.
.......>>>>.<<<<<....<<<<<<<<<<<<<..<<<...<<<.>>>.
.>>>...<<....>>............<<<<<<<<<<<<<....>>>>>>
>.>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<
<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<
<........>>>>.>>>....>>>>>>>>>...<<.......>>......
>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<
<<<<<.<<<<<<<<<...<<<...<<<..<<<<..>>>>..>>>..>>.>
...>>>>>>>.<<<<<<<<<<<......>>>>>>>>>>>...>>>>>>>>
>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>
>...<<<<<<<..<<<<(((...<<<<<<<.....<<<.<<<<<<<..>>
>>>>>>>>..........)))......>>>>>>>>>>>..>>>>>>><<<
<<<..>>>>>>....>>>>>><.<<<<<<<<...<<......<<<<<.<<
....>>.>>>>>...>>>>>>>>>>..>.....>>>>>.<<.....<<..
..<<<<<....>>>>>..<<.....<<<<..<<...<<<<<<...<....
...>.>>>>>>....((<<<.<<...<<<...<<<....>>>......<<
<<..>>>>...>>.>....>>...<<<<<........>>>>>))<<<<<<
<<<....>>><<<<<<<<<<...<<<..<<<<<<<<<<<<<<<<<.....
....>>>>>....<<<<<<...<<<<<<....<<.....>>......>>>
>>>...>>>>>>..>>>>>.>>>>>>>..>>>.........>>>>>>>>>
>>>>>>>.>>>..>>..<<.....>>.>>>>.<<<<.....>>>>..>>.
...<<<<....<<<<<<<<.<<.........<.....<<<<<....<<..
>>.....>>>>><<.<<<<<<...>>>>>>.>>............<<<<<
<<<<..<<<.........>>>..<<<<<<<....<<<<....>>>>....
..>>>>>>>...<<..<<<..<<<<<<<...<<....<<<.<....>.>>
>....>>.>>>>>..>>>>><<<<<<....>>>>>>........<.....
......<<<<<<.....>>>>>>....>.....>>....>>.>>>>>>>.
.>..>>.>>>>>>>>.>>>>>>....>>....<<<<<........>>>>>
...((((((..{{{.........{{{..{{[[[[..<<<<<.<<<<<<<<
..<<<<<<<<<..>>>>>>>>>...<<<<......>>>>.....>>>>>>
>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..
<<...<<<<<<<...<.<<<<..>>>>.>.....<<...(((>>....>>
>>>>>...<<<<<<<<.........>>>>>>>>.<<<....>>>.>>)))
.......((((((.((((.>>>>>>))))))))))..........]]]].
.}}.}}}.......<<.<.<.<<<<<<...<<.......>>...>>>>>>
...>>.>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>
>..>>>.<<<.........>>>....>>>>>..>...>>>.>>....<<<
<<<<....>>>>>>>........}}}.))))))..<<<<<<.<<<..<<<
<<<<<..............>>>>>>>><<<<<...<.<....<<<<<<<<
<<....>>>>>.>>>>>....>.>.>>>>>.<<<...<<<<<<.......
....>>>>>>...>>>..>>>.>>>>>>...<.<<<..............
...............>>>.>...<<.<<<<.......>>>>..<<<<<<<
<<<<....<<<<<<.<..>.>>>>>>...>>>>.>>>>>>>....>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7pwo Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
• Resolution: 2.75 Å
• Binding residue (original residue number in PDB): S2 H3 R4 K5 S7 C9 R10 K11 G12 N13 L14 G15 Y16 L17 P18 R19 K20 R21 T23 R24 R28 K30 T31 K50 C53 H55 H62 S65 Q75 Y92 L99 T104 W106 R117 R120 F123 H124 T128 F130 S131 T132 Y133 R158 K171 Q172 K173 K174 E179 T221 R222 G223 R224 G225 F226 G228 R232 T236 R237 R240 K241 T242 R243 R244 G245 R247 K248 V249 A250 C251 I252 G253 A254 W255 H256 P257 A258 N259 V260 T263 R266 A267 Q269 Y272 H274 R275 T278 N279 K280 G309 V312 N313 G315 T327 C328 R334 R339 L343 H345 R346 N347 F362 G363 H364 R373 R374 F376 Y377
• Binding residue (residue number reindexed from 1): S1 H2 R3 K4 S6 C8 R9 K10 G11 N12 L13 G14 Y15 L16 P17 R18 K19 R20 T22 R23 R27 K29 T30 K49 C52 H54 H61 S64 Q74 Y91 L98 T103 W105 R116 R119 F122 H123 T127 F129 S130 T131 Y132 R157 K170 Q171 K172 K173 E178 T220 R221 G222 R223 G224 F225 G227 R231 T235 R236 R239 K240 T241 R242 R243 G244 R246 K247 V248 A249 C250 I251 G252 A253 W254 H255 P256 A257 N258 V259 T262 R265 A266 Q268 Y271 H273 R274 T277 N278 K279 G308 V311 N312 G314 T326 C327 R333 R338 L342 H344 R345 N346 F361 G362 H363 R372 R373 F375 Y376

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7pwo, PDBe:7pwo, PDBj:7pwo
• PDBsum: 7pwo
• PubMed: 35100413
• UniProt: A8BRZ3