Structure of PDB 8j27 Chain B Binding Site BS01

Receptor Information

>8j27 Chain B (length=264) Species: 3702 (Arabidopsis thaliana)

SRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVI
LVSSGAVGLGRQRLRYRQLVNSSFADLLDGKACAGVGQSSLMAYYETMFD
QLDVTVAQMLVTDSSFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTR
DSTGIFWDNDSLAALLSLELKADLLILLSDVEGLYTGPPSDSTSKLIHTF
IKEKHQDEITFGELGRGGMTAKVKAAVNAAYGGVPVIITSGYAAENISKV
LRGLRVGTLFHQDA

Ligand information

>8j27 Chain F (length=12) Species: 3702 (Arabidopsis thaliana)

YRQLVNSSFADL

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8j27 GK monomer complexes with ADP
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): V76 S78 S79
• Binding residue (residue number reindexed from 1): V70 S72 S73

Enzymatic activity

• Enzyme Commision number: 1.2.1.41: glutamate-5-semialdehyde dehydrogenase.
  2.7.2.11: glutamate 5-kinase.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004349 glutamate 5-kinase activity
• GO:0004350 glutamate-5-semialdehyde dehydrogenase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity
• GO:0016491 oxidoreductase activity
• GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Biological Process

• GO:0006561 proline biosynthetic process
• GO:0009084 glutamine family amino acid biosynthetic process
• GO:0009555 pollen development
• GO:0009793 embryo development ending in seed dormancy
• GO:0016310 phosphorylation
• GO:0055129 L-proline biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0009506 plasmodesma
• GO:0009507 chloroplast

External links

• PDB: RCSB:8j27, PDBe:8j27, PDBj:8j27
• PDBsum: 8j27
• UniProt: P54888|P5CS2_ARATH Delta-1-pyrroline-5-carboxylate synthase B (Gene Name=P5CSB)