Structure of PDB 8foh Chain B Binding Site BS01

Receptor Information

>8foh Chain B (length=406) Species: 4932 (Saccharomyces cerevisiae)

EEKKLYARLYESKLSFYDLPPQEQFEIWAIDRLKILLEIESCLSRNKSIK
EIETIIKPQFQKLLPFNTESLEDRKKDYYSHFILRLCFCRSKELREKFVR
AETFLFKIRFNMLTSTDQTKFVQSLQFISNEEKAELSHQLYQTVSASLQF
QLNLNEEHQRKQYFQQEKFIKLPFENVIELVGNRLVFLKDGYAYLQQLNL
LSNEFASKLNQELIKTYQYLPRLNEDRLLPILNHLSSGYTDEINAQSVWS
EEISSNYPLCIKNLMEGLKKNHHLRYYGRQQLSLFLKGIGLSADEALKFW
SEAFTKEYRYSFRHNYGLEGNRINYKPWDCHTILSKPRPGRGDYHGCPFR
DWSHERLSAELRAQIISVLDSCQKGEYTIACTKVFEMTHHIAHPNLYFER
SRQLQK

Ligand information

>8foh Chain T (length=12)

gctgccgccgcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8foh Molecular choreography of primer synthesis by the eukaryotic Pol alpha-primase.
• Resolution: 4.6 Å
• Binding residue (original residue number in PDB): K393 H401 N408
• Binding residue (residue number reindexed from 1): K306 H314 N321

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003697 single-stranded DNA binding
• GO:0003896 DNA primase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006269 DNA replication, synthesis of primer
• GO:0006270 DNA replication initiation
• GO:0006302 double-strand break repair

Cellular Component

• GO:0005634 nucleus
• GO:0005635 nuclear envelope
• GO:0005658 alpha DNA polymerase:primase complex
• GO:1990077 primosome complex

External links

• PDB: RCSB:8foh, PDBe:8foh, PDBj:8foh
• PDBsum: 8foh
• PubMed: 37344454
• UniProt: P20457|PRI2_YEAST DNA primase large subunit (Gene Name=PRI2)