Structure of PDB 8eiu Chain B Binding Site BS01

Receptor Information

>8eiu Chain B (length=224) Species: 562 (Escherichia coli)

VSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEA
LAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTN
WKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKD
MGGLPDALFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGN
DDAIRAVTLYLGAVAATVREGRSQ

Ligand information

>8eiu Chain A (length=1519)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacga
guggcggacgggugaguaaugucugggaaacugccugauggagggggaua
acuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagg
gggccucuugccaucggaugugcccagaugggauuagcuaguaggugggg
uaacggcucaccuaggcgacgaucccuagcuggucugagaggaugaccag
ccacacuggaacugagacacgguccagacuccuacgggaggcagcagugg
ggaauauugcacaaugggcgcaagccugaugcagccaugccgcguguaug
aagaaggccuucggguuguaaaguacuuucagcggggaggaagggaguaa
aguuaauaccuuugcucauugacguuacccgcagaagaagcaccggcuaa
cuccgugccagcagccgcgguaauacggagggugcaagcguuaaucggaa
uuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugugaaau
ccccgggcucaaccugggaacugcaucugauacuggcaagcuugagucuc
guagagggggguagaauuccagguguagcggugaaaugcguagagaucug
gaggaauaccgguggcgaaggcggcccccuggacgaagacugacgcucag
gugcgaaagcguggggagcaaacaggauuagauacccugguaguccacgc
cguaaacgaugucgacuuggagguugugccggcguggcuuccggagcuaa
cgcguuaagucgaccgccuggggaguacggccgcaagguuaaaacucaaa
ugaauugacgggggcccgcacaagcgguggagcaugugguuuaauucgau
gcaacgcgaagaaccuuaccuggucuugacauccacggaaguuuucagag
augagaaugugccuucgggaaccgugagacaggugcugcauggcugucgu
cagcucguguugugaaauguuggguuaagucccgcaacgagcgcaacccu
uauccuuuguugccagcgguccggccgggaacucaaaggagacugccagu
gauaaacuggaggaagguggggaugacgucaagucaucauggcccuuacg
accagggcuacacacgugcuacaauggcgcauacaaagagaagcgaccuc
gcgagagcaagcggaccucauaaagugcgucguaguccggauuggagucu
gcaacucgacuccaugaagucggaaucgcuaguaaucguggaucagaaug
ccacggugaauacguucccgggccuuguacacaccgcccgucacaccaug
ggaguggguugcaaaagaaguagguagcuuaaccuucgggagggcgcuua
ccacuuugugauucaugacuggggugaagucguaacaagguaaccguagg
ggaaccugcgguuggauca

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<<..<<<<<<<<........>>>>>.>>>>>..
>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<....
.<<<<<......>>>>>......>>.>.....<<<....>>>....<<<<
<<.>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<..
.......>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>
>.>>.<<<<<.<.........>>>>>>.<<<<<..>>>>>...>>>>>>.
........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<<<<.
.....<<<<....>>>>.....>>>>...<.<<<<<......<.<<<<<<
<<.......>>>>>>>>.>........>>>>>....>..<<<<<(((...
<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>....
......<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<.....
.<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<<<
<<...<<<<<<<....<<<<<<<<...<<<......>>>......>>>>>
>>>...........<<....>>.>>>>>>>..>>>>.>>>>...>>>...
>>>>....<<<<<<...<<...<<<<.<.....>.>>>>...>>>>>>>>
..........<<<<<<.<<<<<<<<<<<<..>>>>>>>>>>>>..<<..)
)>>.....>>>>>>.>>>.<<<......<<<<....>>>>....>>>..)
))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<..
......>>..........<<<<<<<......<<<<<<<..<<<<<<<...
.>>>>>>>...<......>..>>>>>.>>.<<<.<<<..<<<<<<.....
..<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.....<<<
<.<<<<<<<...<<..<<<.....>>>>>....>>>>>>>.....<<<<<
.....>>>>>........>>>>.........>>>...>>>>>>>>>...>
>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<<...<<<
....>>>...>>....>>>.....>>>>>>>......<....<<<<<<<.
.......>>>>>>>....>.....>>>>>>....<<<<<<<.........
>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<
<..<<<<<<<<<<<<....<<<<<<.<<<<..<<<..>>>.>>>>>>>>>
>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<
....>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8eiu Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome.
• Resolution: 2.24 Å
• Binding residue (original residue number in PDB): R21 W23 P25 R95 G98 G99 T102 N103 K105 T106 K132 R139 K143 I171 E175 N178
• Binding residue (residue number reindexed from 1): R18 W20 P22 R92 G95 G96 T99 N100 K102 T103 K129 R136 K140 I168 E172 N175

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8eiu, PDBe:8eiu, PDBj:8eiu
• PDBsum: 8eiu
• PubMed: 36660825
• UniProt: P0A7V0|RS2_ECOLI Small ribosomal subunit protein uS2 (Gene Name=rpsB)