Structure of PDB 8c2o Chain B Binding Site BS01

Receptor Information

>8c2o Chain B (length=226) Species: 562 (Escherichia coli)

KRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDARLT
RSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGA
SSAMAKYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLG
SHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLL
GTAAFRQKIATAIAEGVISYFHWFDN

Ligand information

• Ligand ID: ZN
• InChI: InChI=1S/Zn/q+2
• InChIKey: PTFCDOFLOPIGGS-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Zn++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Zn+2]
• Formula: Zn
• Name: ZINC ION
• ChEMBL: CHEMBL1236970
• DrugBank: DB14532
• PDB chain: 8c2o Chain B Residue 301

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c2o Activator-induced conformational changes regulate division-associated peptidoglycan amidases.
• Resolution: 2.35 Å
• Binding residue (original residue number in PDB): H65 E80 H133 D135 E167
• Binding residue (residue number reindexed from 1): H10 E25 H78 D80 E112
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 3.5.1.28: N-acetylmuramoyl-L-alanine amidase.

Gene Ontology

Molecular Function

• GO:0008270 zinc ion binding
• GO:0008745 N-acetylmuramoyl-L-alanine amidase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0009253 peptidoglycan catabolic process
• GO:0051301 cell division
• GO:0071236 cellular response to antibiotic
• GO:0071555 cell wall organization

Cellular Component

• GO:0030288 outer membrane-bounded periplasmic space
• GO:0042597 periplasmic space

External links

• PDB: RCSB:8c2o, PDBe:8c2o, PDBj:8c2o
• PDBsum: 8c2o
• PubMed: 37276423
• UniProt: P36548|AMIA_ECOLI N-acetylmuramoyl-L-alanine amidase AmiA (Gene Name=amiA)