Structure of PDB 7z4j Chain B Binding Site BS01

Receptor Information
>7z4j Chain B (length=1345) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL
EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI
NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN
FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF
FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK
QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN
LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDL
LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKII
KDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQL
KRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS
LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVM
GRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPV
ENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDS
IDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLT
KAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIR
EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKY
PKLESEFVYGDYIGKATAKYFFYSNIMNFFKTEITIRKRPLIETNGETGE
IVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIA
RKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMER
SSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQ
KGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIE
QISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG
Ligand information
>7z4j Chain A (length=98) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gacgcauaaagaugagacgcguuuuagagcuagaaauagcaaguuaaaau
aaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuu
....................<<<<<<..<<<<....>>>>....>>>>>>
..<<.....>>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7z4j R-loop formation and conformational activation mechanisms of Cas9.
Resolution2.99 Å
Binding residue
(original residue number in PDB)		K30 K33 L35 R40 I43 K44 N46 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 N77 Y81 L101 S104 F105 R115 H116 I122 E125 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 K336 R340 F351 F352 Y359 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 L455 A456 R457 N459 S460 F462 W464 T472 I473 P475 F491 Y515 N588 R661 M694 S719 H721 S730 P731 L738 R753 S777 R778 E779 R780 N803 Q807 K848 D850 S851 I852 K918 R919 V922 Y981 Y1010 G1011 Y1013 P1090 Q1091 V1100 T1102 G1103 F1105 I1110 P1112 K1113 R1122 K1123 K1124 K1130 Y1131 F1134 M1169 E1225 A1227 Q1272 H1349 Q1350 Y1356
Binding residue
(residue number reindexed from 1)		K29 K32 L34 R39 I42 K43 N45 E56 T57 A58 T61 R62 K64 R65 R68 R69 R70 Y71 R73 R74 N76 Y80 L100 S103 F104 R114 H115 I121 E124 H128 T133 I134 H159 K162 F163 R164 G165 Y324 H327 H328 K335 R339 F350 F351 Y358 I362 Q401 R402 T403 F404 N406 G407 F445 R446 I447 Y449 G452 L454 A455 R456 N458 S459 F461 W463 T471 I472 P474 F490 Y514 N587 R660 M693 S718 H720 S729 P730 L737 R752 S776 R777 E778 R779 N802 Q806 K847 D849 S850 I851 K917 R918 V921 Y980 Y1009 G1010 Y1012 P1069 Q1070 V1079 T1081 G1082 F1084 I1089 P1091 K1092 R1101 K1102 K1103 K1109 Y1110 F1113 M1148 E1204 A1206 Q1251 H1328 Q1329 Y1335
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:7z4j, PDBe:7z4j, PDBj:7z4j
PDBsum7z4j
PubMed36002571
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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